##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AseI AsnI AviII BclI BfrI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI Bst1107I BstZ17I CelII ClaI
CpoI DraI DraIII EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI MamI MfeI MluI MroI
MunI NdeI NotI NruI NsiI
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1683 GACGTC GACGT^C
Acc65I 69 GGTACC G^GTACC
AhdI 1318 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1024 GTGCAC G^TGCAC
AocI 2450 CCTGAGG CC^T[ACGT]AGG
ApaLI 1024 GTGCAC G^TGCAC
AscI 839 GGCGCGCC GG^CGCGCC
Asp700I 864 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 69 GGTACC G^GTACC
AspEI 1318 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3007 CCTAGG C^CTAGG
BciVI 4151 GGATAC GTATCC(6/5)
BglII 5019 AGATCT A^GATCT
BlnI 3007 CCTAGG C^CTAGG
BstBI 1603 TTCGAA TT^CGAA
Bsu36I 2450 CCTGAGG CC^T[ACGT]AGG
DrdI 970 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1318 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2450 CCTGAGG CC^T[ACGT]AGG
EcoRI 3374 GAATTC G^AATTC
FseI 1567 GGCCGGCC GGCCGG^CC
KpnI 69 GGTACC GGTAC^C
NaeI 1568 GCCGGC GCC^GGC
NgoMIV 1568 GCCGGC G^CCGGC
NheI 1446 GCTAGC G^CTAGC
NspI 905 ACATGC [GA]CATG^[CT]
NspV 1603 TTCGAA TT^CGAA
PflMI 4955 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfuI 1603 TTCGAA TT^CGAA
SspI 4319 AATATT AAT^ATT
Van91I 4955 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 864 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 3374 GAATTC [GA]^AATT[CT]
4487 AAATTC
AflIII 2340 ACGTGT A^C[GA][CT]GT
4589 ACACGT
ApoI 3374 GAATTC [GA]^AATT[CT]
4487 AAATTC
AspI 1635 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2183 GACCTCGTC
BamHI 503 GGATCC G^GATCC
1193 GGATCC
BbsI 273 GAAGAC GAAGAC(2/6)
3112 GAAGAC
BcgI 2119 CGAAGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2251 GCACCCCGGTCG
BpuAI 273 GAAGAC GAAGAC(2/6)
3112 GAAGAC
BspHI 767 TCATGA T^CATGA
1370 TCATGA
BstAPI 651 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
900 GCAGGACATGC
BstEII 4414 GGTGACC G^GT[ACGT]ACC
4441 GGTCACC
BstPI 4414 GGTGACC G^GT[ACGT]ACC
4441 GGTCACC
EcoNI 3137 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3263 CCTTCCCCAGG
EcoO65I 4414 GGTGACC G^GT[ACGT]ACC
4441 GGTCACC
MseI 3080 TTAA T^TAA
4815 TTAA
PaeR7I 2731 CTCGAG C^TCGAG
3968 CTCGAG
PflFI 1635 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2183 GACCTCGTC
PvuII 307 CAGCTG CAG^CTG
2772 CAGCTG
RcaI 767 TCATGA T^CATGA
1370 TCATGA
SapI 864 GAAGAGC GCTCTTC(1/4)
2398 GAAGAGC
Tru9I 3080 TTAA T^TAA
4815 TTAA
Tth111I 1635 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2183 GACCTCGTC
XhoI 2731 CTCGAG C^TCGAG
3968 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PleI PmaCI
PmlI PpuMI PstI RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI