##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbrPI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
ClaI CpoI DrdI Eco47III EcoT22I
FbaI FseI FspI HindIII KpnI
KspI MfeI MluI MroI MunI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3149 GTAGAC GT^[AC][GT]AC
AccII 219 CGCG CG^CG
AflIII 4079 ACATGT A^C[GA][CT]GT
AspI 1872 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3267 TGGCCA TGG^CCA
BbeI 3250 GGCGCC GGCGC^C
BcgI 2583 CGATCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 107 ACCGGA [AT]^CCGG[AT]
BspHI 4486 TCATGA T^CATGA
BspLU11I 4079 ACATGT A^CATGT
BsrBI 157 GAGCGG CCGCTC(-3/-3)
BssSI 461 CACGAG CACGAG(-5/-1)
BstEII 1647 GGTTACC G^GT[ACGT]ACC
BstPI 1647 GGTTACC G^GT[ACGT]ACC
BstUI 219 CGCG CG^CG
CfoI 3251 GCGC GCG^C
DraI 4280 TTTAAA TTT^AAA
DraIII 4009 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1647 GGTTACC G^GT[ACGT]ACC
HaeII 3250 GGCGCC [GA]GCGC^[CT]
HhaI 3251 GCGC GCG^C
HinP1I 3251 GCGC G^CGC
HpaI 2827 GTTAAC GTT^AAC
KasI 3250 GGCGCC G^GCGCC
MaeII 1801 ACGT A^CGT
MluNI 3267 TGGCCA TGG^CCA
MscI 3267 TGGCCA TGG^CCA
MvnI 219 CGCG CG^CG
NaeI 135 GCCGGC GCC^GGC
NarI 3250 GGCGCC GG^CGCC
NdeI 2886 CATATG CA^TATG
NgoMIV 135 GCCGGC G^CCGGC
NheI 4171 GCTAGC G^CTAGC
PflFI 1872 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 4089 CTGCAG CTGCA^G
RcaI 4486 TCATGA T^CATGA
SacI 1918 GAGCTC GAGCT^C
SapI 2626 GAAGAGC GCTCTTC(1/4)
ScaI 420 AGTACT AGT^ACT
SfoI 3250 GGCGCC GGC^GCC
TaqI 1008 TCGA T^CGA
Tth111I 1872 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1008 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 138 GGCGTC G[GA]^CG[CT]C
3250 GGCGCC
AhdI 2011 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4403 GACCAGAAGTC
AlwNI 124 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1316 CAGGAGCTG
AocI 1037 CCTCAGG CC^T[ACGT]AGG
2905 CCTCAGG
Asp700I 1088 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2857 GAATCCATTC
AspEI 2011 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4403 GACCAGAAGTC
AvaI 8 CCCGGG C^[CT]CG[GA]G
3052 CCCGGG
BbvCI 430 GCTGAGG CCTCAGC(-5/-2)
3202 GCTGAGG
BciVI 3941 GTATCC GTATCC(6/5)
4073 GTATCC
BsaBI 2749 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3577 GATGATGATC
BsaHI 138 GGCGTC G[GA]^CG[CT]C
3250 GGCGCC
BsiEI 160 CGGCCG CG[GA][CT]^CG
212 CGGCCG
BsmI 4041 GAATGC GAATGC(1/-1)
2433 GCATTC
BsoBI 8 CCCGGG C^[CT]CG[GA]G
3052 CCCGGG
BspMI 2015 GCAGGT ACCTGC(4/8)
4509 GCAGGT
BsrDI 2124 CATTGC GCAATG(2/0)
3231 CATTGC
BsrFI 135 GCCGGC [GA]^CCGG[CT]
3067 GCCGGT
Bsu36I 1037 CCTCAGG CC^T[ACGT]AGG
2905 CCTCAGG
Cfr10I 135 GCCGGC [GA]^CCGG[CT]
3067 GCCGGT
EagI 160 CGGCCG C^GGCCG
212 CGGCCG
Eam1105I 2011 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4403 GACCAGAAGTC
EclXI 160 CGGCCG C^GGCCG
212 CGGCCG
Eco52I 160 CGGCCG C^GGCCG
212 CGGCCG
Eco81I 1037 CCTCAGG CC^T[ACGT]AGG
2905 CCTCAGG
EcoRI 244 GAATTC G^AATTC
4522 GAATTC
HgaI 217 GACGC GACGC(5/10)
139 GCGTC
Hin1I 138 GGCGTC G[GA]^CG[CT]C
3250 GGCGCC
HincII 2674 GTTGAC GT[CT]^[GA]AC
2827 GTTAAC
HindII 2674 GTTGAC GT[CT]^[GA]AC
2827 GTTAAC
MamI 2749 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3577 GATGATGATC
NcoI 1001 CCATGG C^CATGG
2461 CCATGG
NspI 4079 ACATGT [GA]CATG^[CT]
4541 GCATGT
PvuII 1856 CAGCTG CAG^CTG
3326 CAGCTG
SmaI 8 CCCGGG CCC^GGG
3052 CCCGGG
SmlI 424 CTCAAG C^T[CT][GA]AG
1672 CTCAAG
SspI 1978 AATATT AAT^ATT
4250 AATATT
XmaCI 8 CCCGGG C^CCGGG
3052 CCCGGG
XmaI 8 CCCGGG C^CCGGG
3052 CCCGGG
XmnI 1088 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2857 GAATCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII