##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspI AviII AvrII BamHI
BclI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
KpnI KspI MamI MluI MroI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SgrAI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XcmI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 79 GACGTC GACGT^C
AccI 3269 GTCTAC GT^[AC][GT]AC
AccII 5403 CGCG CG^CG
AocI 596 CCTCAGG CC^T[ACGT]AGG
BanI 3333 GGCGCC G^G[CT][GA]CC
BbeI 3333 GGCGCC GGCGC^C
BbrPI 5018 CACGTG CAC^GTG
BcgI 5361 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2227 GGATAC GTATCC(6/5)
BsmBI 1279 GAGACG CGTCTC(1/5)
BspHI 1816 TCATGA T^CATGA
BspLU11I 977 ACATGT A^CATGT
BstAPI 4117 GCAGGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5403 CGCG CG^CG
Bsu36I 596 CCTCAGG CC^T[ACGT]AGG
Eco81I 596 CCTCAGG CC^T[ACGT]AGG
EcoNI 1627 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 3333 GGCGCC [GA]GCGC^[CT]
HpaI 2764 GTTAAC GTT^AAC
KasI 3333 GGCGCC G^GCGCC
MvnI 5403 CGCG CG^CG
NarI 3333 GGCGCC GG^CGCC
PaeR7I 731 CTCGAG C^TCGAG
PmaCI 5018 CACGTG CAC^GTG
PmeI 4379 GTTTAAAC GTTT^AAAC
PmlI 5018 CACGTG CAC^GTG
PpuMI 138 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1816 TCATGA T^CATGA
SacI 111 GAGCTC GAGCT^C
ScaI 2996 AGTACT AGT^ACT
SfoI 3333 GGCGCC GGC^GCC
SmaI 5349 CCCGGG CCC^GGG
SpeI 916 ACTAGT A^CTAGT
XhoI 731 CTCGAG C^TCGAG
XmaCI 5349 CCCGGG C^CCGGG
XmaI 5349 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 79 GACGTC G[GA]^CG[CT]C
3333 GGCGCC
AhdI 125 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3596 GACCATGAGTC
AseI 2873 ATTAAT AT^TAAT
5132 ATTAAT
AsnI 2873 ATTAAT AT^TAAT
5132 ATTAAT
AspEI 125 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3596 GACCATGAGTC
AspHI 111 GAGCTC G[AT]GC[AT]^C
2108 GAGCAC
BalI 4022 TGGCCA TGG^CCA
5464 TGGCCA
BbvCI 5372 CCTCAGC CCTCAGC(-5/-2)
44 GCTGAGG
BglI 53 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4326 GCCTATAGGGC
BglII 1712 AGATCT A^GATCT
3842 AGATCT
BsaHI 79 GACGTC G[GA]^CG[CT]C
3333 GGCGCC
BsiHKAI 111 GAGCTC G[AT]GC[AT]^C
2108 GAGCAC
BspMI 358 ACCTGC ACCTGC(4/8)
3476 ACCTGC
BstBI 2970 TTCGAA TT^CGAA
2988 TTCGAA
DraII 138 AGGTCCC [GA]G^G[ACGT]CC[CT]
4332 AGGGCCT
Eam1105I 125 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3596 GACCATGAGTC
EcoO109I 138 AGGTCCC [GA]G^G[ACGT]CC[CT]
4332 AGGGCCT
EcoRI 157 GAATTC G^AATTC
2967 GAATTC
Hin1I 79 GACGTC G[GA]^CG[CT]C
3333 GGCGCC
HincII 82 GTCAAC GT[CT]^[GA]AC
2764 GTTAAC
HindII 82 GTCAAC GT[CT]^[GA]AC
2764 GTTAAC
MfeI 663 CAATTG C^AATTG
2721 CAATTG
MluNI 4022 TGGCCA TGG^CCA
5464 TGGCCA
MscI 4022 TGGCCA TGG^CCA
5464 TGGCCA
MunI 663 CAATTG C^AATTG
2721 CAATTG
NcoI 869 CCATGG C^CATGG
2622 CCATGG
NspV 2970 TTCGAA TT^CGAA
2988 TTCGAA
PshBI 2873 ATTAAT AT^TAAT
5132 ATTAAT
PvuII 170 CAGCTG CAG^CTG
3535 CAGCTG
SapI 1151 GAAGAGC GCTCTTC(1/4)
2249 GAAGAGC
SfuI 2970 TTCGAA TT^CGAA
2988 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI Asp700I AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI