##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
Aor51HI AscI Asp718I AspI BalI
BamHI BanI BbeI BbrPI BfrI
BsmBI BspDI BsrBI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DrdI Eco47III
EcoO65I EcoRI EcoT22I FseI KasI
KpnI MfeI MluI MluNI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PstI PvuI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI Sse8387I SwaI Tth111I
Van91I XbaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 2573 TCCGGA T^CCGGA
AclI 3396 AACGTT AA^CGTT
AcyI 3303 GACGCC G[GA]^CG[CT]C
AhdI 1523 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 636 GTGCAC G^TGCAC
AlwNI 1822 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 94 GGGCCC GGGCC^C
ApaLI 636 GTGCAC G^TGCAC
AseI 3203 ATTAAT AT^TAAT
AsnI 3203 ATTAAT AT^TAAT
AspEI 1523 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3281 TGCGCA TGC^GCA
AvrII 4597 CCTAGG C^CTAGG
BbvCI 3964 GCTGAGG CCTCAGC(-5/-2)
BcgI 3789 CGAAAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1864 GGATAC GTATCC(6/5)
BglII 1264 AGATCT A^GATCT
BlnI 4597 CCTAGG C^CTAGG
BlpI 4089 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4089 GCTGAGC GC^T[ACGT]AGC
BsaAI 1679 TACGTA [CT]AC^GT[GA]
BsaBI 1564 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3303 GACGCC G[GA]^CG[CT]C
BsaWI 2573 TCCGGA [AT]^CCGG[AT]
BseAI 2573 TCCGGA T^CCGGA
BsgI 779 GTGCAG GTGCAG(16/14)
BsiWI 79 CGTACG C^GTACG
BspEI 2573 TCCGGA T^CCGGA
BsrFI 4548 GCCGGT [GA]^CCGG[CT]
CelII 4089 GCTGAGC GC^T[ACGT]AGC
Cfr10I 4548 GCCGGT [GA]^CCGG[CT]
Eam1105I 1523 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2680 CCTTTACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1366 GATATC GAT^ATC
FspI 3281 TGCGCA TGC^GCA
HaeII 2795 AGCGCC [GA]GCGC^[CT]
Hin1I 3303 GACGCC G[GA]^CG[CT]C
HpaI 1936 GTTAAC GTT^AAC
MamI 1564 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2573 TCCGGA T^CCGGA
PaeR7I 1762 CTCGAG C^TCGAG
PpuMI 2520 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3203 ATTAAT AT^TAAT
Psp1406I 3396 AACGTT AA^CGTT
SapI 991 GAAGAGC GCTCTTC(1/4)
SexAI 1524 ACCTGGT A^CC[AT]GGT
SfcI 1731 CTACAG C^T[GA][CT]AG
SmaI 91 CCCGGG CCC^GGG
SmlI 1762 CTCGAG C^T[CT][GA]AG
SnaBI 1679 TACGTA TAC^GTA
SpeI 1032 ACTAGT A^CTAGT
TfiI 3068 GATTC G^A[AT]TC
XcmI 2458 CCATGGCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1762 CTCGAG C^TCGAG
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 140 CGCG CG^CG
192 CGCG
AocI 2281 CCTCAGG CC^T[ACGT]AGG
2421 CCTGAGG
Asp700I 1987 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3690 GAAGATTTTC
AvaI 91 CCCGGG C^[CT]CG[GA]G
1762 CTCGAG
BglI 390 GCCTTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3955 GCCTCGTTGGC
BsiEI 136 CGGCCG CG[GA][CT]^CG
4546 CGGCCG
BsoBI 91 CCCGGG C^[CT]CG[GA]G
1762 CTCGAG
BspLU11I 1047 ACATGT A^CATGT
2950 ACATGT
BspMI 86 ACCTGC ACCTGC(4/8)
3239 ACCTGC
BsrDI 2499 CATTGC GCAATG(2/0)
4438 GCAATG
BstUI 140 CGCG CG^CG
192 CGCG
Bsu36I 2281 CCTCAGG CC^T[ACGT]AGG
2421 CCTGAGG
CfoI 2796 GCGC GCG^C
3282 GCGC
DraII 2028 AGGGCCT [GA]G^G[ACGT]CC[CT]
2520 GGGTCCT
DraIII 639 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
641 CACTGTGTG
EagI 136 CGGCCG C^GGCCG
4546 CGGCCG
EarI 871 GAAGAG CTCTTC(1/4)
991 GAAGAG
EclXI 136 CGGCCG C^GGCCG
4546 CGGCCG
Eco52I 136 CGGCCG C^GGCCG
4546 CGGCCG
Eco81I 2281 CCTCAGG CC^T[ACGT]AGG
2421 CCTGAGG
EcoO109I 2028 AGGGCCT [GA]G^G[ACGT]CC[CT]
2520 GGGTCCT
HhaI 2796 GCGC GCG^C
3282 GCGC
HinP1I 2796 GCGC G^CGC
3282 GCGC
Ksp632I 871 GAAGAG CTCTTC(1/4)
991 GAAGAG
KspI 139 CCGCGG CCGC^GG
191 CCGCGG
MvnI 140 CGCG CG^CG
192 CGCG
NcoI 687 CCATGG C^CATGG
2458 CCATGG
NdeI 204 CATATG CA^TATG
4398 CATATG
PvuII 818 CAGCTG CAG^CTG
2213 CAGCTG
SacI 552 GAGCTC GAGCT^C
2203 GAGCTC
SacII 139 CCGCGG CCGC^GG
191 CCGCGG
SspI 3557 AATATT AAT^ATT
3613 AATATT
StuI 1176 AGGCCT AGG^CCT
1989 AGGCCT
XmnI 1987 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3690 GAAGATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EaeI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SphI SspBI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII