##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AclI AcsI AflIII Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AviII BamHI BbeI
BcgI BciVI BsaBI BsiWI BspDI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eco47III EcoO65I EcoRI EcoRV FspI
HaeII KasI KpnI MamI MluI
NarI NdeI NheI NotI NruI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SfoI SfuI SnaBI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1579 GACGTC GACGT^C
AflII 148 CTTAAG C^TTAAG
AhdI 301 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2745 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 301 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3184 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1183 CACGTG CAC^GTG
BfrI 148 CTTAAG C^TTAAG
Bsp1407I 2682 TGTACA T^GTACA
BsrGI 2682 TGTACA T^GTACA
Eam1105I 301 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4484 ATGCAT ATGCA^T
HindIII 4498 AAGCTT A^AGCTT
HpaI 4175 GTTAAC GTT^AAC
MfeI 2841 CAATTG C^AATTG
MunI 2841 CAATTG C^AATTG
NsiI 4484 ATGCAT ATGCA^T
PaeR7I 2283 CTCGAG C^TCGAG
PflFI 3184 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1183 CACGTG CAC^GTG
PmlI 1183 CACGTG CAC^GTG
SexAI 4234 ACCAGGT A^CC[AT]GGT
SgrAI 1940 CACCGGTG C[GA]^CCGG[CT]G
SpeI 4699 ACTAGT A^CTAGT
SspBI 2682 TGTACA T^GTACA
SspI 2362 AATATT AAT^ATT
Tth111I 3184 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2283 CTCGAG C^TCGAG
XmnI 2745 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1210 GTCTAC GT^[AC][GT]AC
1334 GTCTAC
AccIII 904 TCCGGA T^CCGGA
1238 TCCGGA
AcyI 24 GACGCC G[GA]^CG[CT]C
1579 GACGTC
AgeI 527 ACCGGT A^CCGGT
1941 ACCGGT
AlwI 3404 GATCC GGATC(4/5)
1241 GGATC
AvrII 2754 CCTAGG C^CTAGG
4954 CCTAGG
BbvCI 414 CCTCAGC CCTCAGC(-5/-2)
1548 CCTCAGC
BclI 4431 TGATCA T^GATCA
4477 TGATCA
BglII 881 AGATCT A^GATCT
4395 AGATCT
BlnI 2754 CCTAGG C^CTAGG
4954 CCTAGG
BsaAI 1168 CACGTA [CT]AC^GT[GA]
1183 CACGTG
BsaHI 24 GACGCC G[GA]^CG[CT]C
1579 GACGTC
BseAI 904 TCCGGA T^CCGGA
1238 TCCGGA
BsiEI 772 CGGCCG CG[GA][CT]^CG
1638 CGGCCG
BsmBI 22 GAGACG CGTCTC(1/5)
3350 GAGACG
BsmI 3609 GAATGC GAATGC(1/-1)
4812 GCATTC
BspEI 904 TCCGGA T^CCGGA
1238 TCCGGA
BsrDI 246 CATTGC GCAATG(2/0)
3217 GCAATG
BstYI 881 AGATCT [GA]^GATC[CT]
4395 AGATCT
DraIII 1672 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2735 CACTATGTG
EagI 772 CGGCCG C^GGCCG
1638 CGGCCG
EclXI 772 CGGCCG C^GGCCG
1638 CGGCCG
Eco52I 772 CGGCCG C^GGCCG
1638 CGGCCG
FbaI 4431 TGATCA T^GATCA
4477 TGATCA
FseI 1525 GGCCGGCC GGCCGG^CC
1639 GGCCGGCC
Hin1I 24 GACGCC G[GA]^CG[CT]C
1579 GACGTC
KspI 328 CCGCGG CCGC^GG
1647 CCGCGG
MflI 881 AGATCT [GA]^GATC[CT]
4395 AGATCT
MroI 904 TCCGGA T^CCGGA
1238 TCCGGA
NaeI 1526 GCCGGC GCC^GGC
1640 GCCGGC
NgoMIV 1526 GCCGGC G^CCGGC
1640 GCCGGC
NspI 3001 GCATGC [GA]CATG^[CT]
3456 GCATGC
PinAI 527 ACCGGT A^CCGGT
1941 ACCGGT
SacII 328 CCGCGG CCGC^GG
1647 CCGCGG
SapI 1314 GAAGAGC GCTCTTC(1/4)
4143 GCTCTTC
SfiI 1065 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3768 GGCCCTGTGGGCC
SmaI 1076 CCCGGG CCC^GGG
2874 CCCGGG
SphI 3001 GCATGC GCATG^C
3456 GCATGC
TaqI 2284 TCGA T^CGA
3384 TCGA
TthHB8I 2284 TCGA T^CGA
3384 TCGA
XhoII 881 AGATCT [GA]^GATC[CT]
4395 AGATCT
XmaCI 1076 CCCGGG C^CCGGG
2874 CCCGGG
XmaI 1076 CCCGGG C^CCGGG
2874 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI