##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI BbrPI BclI BfrI
BglII BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SfuI SgrAI SnaBI SspBI SspI
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1483 GTCGAC GT^[AC][GT]AC
AcsI 869 GAATTC [GA]^AATT[CT]
ApoI 869 GAATTC [GA]^AATT[CT]
AviII 2451 TGCGCA TGC^GCA
BbeI 2233 GGCGCC GGCGC^C
BcgI 2131 CGAGAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2177 GGATAC GTATCC(6/5)
BsmI 3667 GCATTC GAATGC(1/-1)
BspHI 1554 TCATGA T^CATGA
BssSI 2897 CACGAG CACGAG(-5/-1)
DrdI 2324 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 869 GAATTC G^AATTC
FspI 2451 TGCGCA TGC^GCA
HincII 1483 GTCGAC GT[CT]^[GA]AC
HindII 1483 GTCGAC GT[CT]^[GA]AC
KasI 2233 GGCGCC G^GCGCC
NarI 2233 GGCGCC GG^CGCC
PaeR7I 101 CTCGAG C^TCGAG
PpuMI 2637 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2952 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1554 TCATGA T^CATGA
SalI 1483 GTCGAC G^TCGAC
SbfI 285 CCTGCAGG CCTGCA^GG
ScaI 3733 AGTACT AGT^ACT
SexAI 4168 ACCAGGT A^CC[AT]GGT
SfiI 3334 GGCCGAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2233 GGCGCC GGC^GCC
SpeI 1714 ACTAGT A^CTAGT
Sse8387I 285 CCTGCAGG CCTGCA^GG
StuI 4861 AGGCCT AGG^CCT
XhoI 101 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 1395 ACACGT A^C[GA][CT]GT
1729 ACGTGT
AvrII 1423 CCTAGG C^CTAGG
4627 CCTAGG
BlnI 1423 CCTAGG C^CTAGG
4627 CCTAGG
BlpI 1124 GCTGAGC GC^T[ACGT]AGC
4873 GCTGAGC
Bpu1102I 1124 GCTGAGC GC^T[ACGT]AGC
4873 GCTGAGC
BsgI 377 CTGCAC GTGCAG(16/14)
3230 GTGCAG
BspMI 2221 GCAGGT ACCTGC(4/8)
3278 GCAGGT
BsrBI 988 GAGCGG CCGCTC(-3/-3)
1127 GAGCGG
CelII 1124 GCTGAGC GC^T[ACGT]AGC
4873 GCTGAGC
DraIII 2416 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4999 CACTGTGTG
KspI 365 CCGCGG CCGC^GG
1766 CCGCGG
NaeI 299 GCCGGC GCC^GGC
3379 GCCGGC
NgoMIV 299 GCCGGC G^CCGGC
3379 GCCGGC
SacII 365 CCGCGG CCGC^GG
1766 CCGCGG
Tsp509I 870 AATT ^AATT
3820 AATT
XbaI 3589 TCTAGA T^CTAGA
4399 TCTAGA
XcmI 2841 CCATTGGAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4507 CCAGGAGGGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI