##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AflIII AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BcgI BciVI BglII BlnI BpmI
BpuAI BsaAI BseRI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BspMI BsrDI
BsrGI BstAPI BstBI BstEII BstPI
ClaI CpoI DraI Eam1105I Eco47III
EcoO65I EcoRI EcoRV FspI HaeII
HincII HindII HpaI KasI KpnI
MaeII MfeI MluI MslI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 2601 GTATAC GT^[AC][GT]AC
AccIII 414 TCCGGA T^CCGGA
AcyI 422 GGCGTC G[GA]^CG[CT]C
AflII 1747 CTTAAG C^TTAAG
AgeI 1250 ACCGGT A^CCGGT
ApaI 237 GGGCCC GGGCC^C
Asp700I 1618 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2115 GGATCC G^GATCC
BanI 2464 GGTGCC G^G[CT][GA]CC
BbvCI 2323 CCTCAGC CCTCAGC(-5/-2)
BclI 2774 TGATCA T^GATCA
BfrI 1747 CTTAAG C^TTAAG
BlpI 2431 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2431 GCTCAGC GC^T[ACGT]AGC
BsaBI 1654 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 422 GGCGTC G[GA]^CG[CT]C
BsaI 963 GGTCTC GGTCTC(1/5)
BseAI 414 TCCGGA T^CCGGA
BsiEI 76 CGGCCG CG[GA][CT]^CG
BsmI 2669 GCATTC GAATGC(1/-1)
BspEI 414 TCCGGA T^CCGGA
BspHI 654 TCATGA T^CATGA
BssHII 3 GCGCGC G^CGCGC
Bst1107I 2601 GTATAC GTA^TAC
BstXI 2852 CCATTAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2601 GTATAC GTA^TAC
CelII 2431 GCTCAGC GC^T[ACGT]AGC
DraIII 2624 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1291 GACTTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 76 CGGCCG C^GGCCG
EclXI 76 CGGCCG C^GGCCG
Eco52I 76 CGGCCG C^GGCCG
EcoT22I 2667 ATGCAT ATGCA^T
FbaI 2774 TGATCA T^GATCA
FseI 354 GGCCGGCC GGCCGG^CC
Hin1I 422 GGCGTC G[GA]^CG[CT]C
HphI 2167 GGTGA GGTGA(8/7)
MamI 1654 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 414 TCCGGA T^CCGGA
NsiI 2667 ATGCAT ATGCA^T
PinAI 1250 ACCGGT A^CCGGT
RcaI 654 TCATGA T^CATGA
SexAI 2625 ACCAGGT A^CC[AT]GGT
SmaI 313 CCCGGG CCC^GGG
SpeI 2217 ACTAGT A^CTAGT
StuI 1842 AGGCCT AGG^CCT
XmaCI 313 CCCGGG C^CCGGG
XmaI 313 CCCGGG C^CCGGG
XmnI 1618 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1449 AAATTT [GA]^AATT[CT]
1711 GAATTT
AocI 349 CCTCAGG CC^T[ACGT]AGG
580 CCTGAGG
ApoI 1449 AAATTT [GA]^AATT[CT]
1711 GAATTT
BsgI 2636 CTGCAC GTGCAG(16/14)
2315 GTGCAG
BsrBI 1923 CCGCTC CCGCTC(-3/-3)
64 GAGCGG
BssSI 1929 CACGAG CACGAG(-5/-1)
1815 CTCGTG
Bsu36I 349 CCTCAGG CC^T[ACGT]AGG
580 CCTGAGG
DraII 317 GGGGCCT [GA]G^G[ACGT]CC[CT]
345 AGGGCCT
Eco81I 349 CCTCAGG CC^T[ACGT]AGG
580 CCTGAGG
EcoO109I 317 GGGGCCT [GA]G^G[ACGT]CC[CT]
345 AGGGCCT
HindIII 477 AAGCTT A^AGCTT
550 AAGCTT
NaeI 355 GCCGGC GCC^GGC
434 GCCGGC
NcoI 389 CCATGG C^CATGG
1094 CCATGG
NgoMIV 355 GCCGGC G^CCGGC
434 GCCGGC
PvuII 784 CAGCTG CAG^CTG
2778 CAGCTG
SacI 2170 GAGCTC GAGCT^C
2787 GAGCTC
SapI 2172 GCTCTTC GCTCTTC(1/4)
2718 GCTCTTC
SfiI 68 GGCCCCTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
229 GGCCTGTCGGGCC
TaqI 984 TCGA T^CGA
2455 TCGA
Tsp45I 1012 GTCAC ^GT[GC]AC
2258 GTGAC
TthHB8I 984 TCGA T^CGA
2455 TCGA
XcmI 1028 CCAGGGATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2330 CCAAAGAAACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BfaI BglI BmyI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI Ksp632I
KspI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI Van91I XhoII