##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccI AccIII AcsI AflIII AgeI
ApoI AseI AsnI AviII BamHI
BcgI BclI BseAI BsiWI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstZ17I CpoI
DraI EcoRI EcoRV EcoT22I FbaI
FspI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1974 GGTACC G^GTACC
AclI 3090 AACGTT AA^CGTT
AflII 3006 CTTAAG C^TTAAG
AscI 206 GGCGCGCC GG^CGCGCC
Asp718I 1974 GGTACC G^GTACC
AvrII 1978 CCTAGG C^CTAGG
BfrI 3006 CTTAAG C^TTAAG
BglII 678 AGATCT A^GATCT
BlnI 1978 CCTAGG C^CTAGG
BsaBI 679 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2031 GAGACC GGTCTC(1/5)
BsaWI 2559 TCCGGT [AT]^CCGG[AT]
BsmBI 1780 CGTCTC CGTCTC(1/5)
BspDI 886 ATCGAT AT^CGAT
BssSI 3378 CTCGTG CACGAG(-5/-1)
BstEII 2632 GGTCACC G^GT[ACGT]ACC
BstPI 2632 GGTCACC G^GT[ACGT]ACC
ClaI 886 ATCGAT AT^CGAT
EcoNI 1434 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2632 GGTCACC G^GT[ACGT]ACC
FseI 736 GGCCGGCC GGCCGG^CC
HindIII 503 AAGCTT A^AGCTT
HpaI 2951 GTTAAC GTT^AAC
KpnI 1974 GGTACC GGTAC^C
MamI 679 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 1159 CTCGAG C^TCGAG
PshAI 2626 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3090 AACGTT AA^CGTT
SexAI 1439 ACCAGGT A^CC[AT]GGT
TfiI 393 GAATC G^A[AT]TC
XhoI 1159 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 1735 GTGCAC G^TGCAC
2551 GTGCAC
AlwNI 626 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2015 CAGGACCTG
ApaLI 1735 GTGCAC G^TGCAC
2551 GTGCAC
Asp700I 241 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2842 GAAGCACTTC
BbeI 170 GGCGCC GGCGC^C
2441 GGCGCC
BbrPI 1738 CACGTG CAC^GTG
2837 CACGTG
BciVI 1788 GGATAC GTATCC(6/5)
472 GTATCC
BfaI 1979 CTAG C^TAG
2887 CTAG
BlpI 631 GCTGAGC GC^T[ACGT]AGC
3127 GCTGAGC
Bpu1102I 631 GCTGAGC GC^T[ACGT]AGC
3127 GCTGAGC
BsaAI 1738 CACGTG [CT]AC^GT[GA]
2837 CACGTG
BsiEI 132 CGGCCG CG[GA][CT]^CG
672 CGGCCG
BspMI 2523 ACCTGC ACCTGC(4/8)
2461 GCAGGT
BstYI 678 AGATCT [GA]^GATC[CT]
1587 AGATCC
CelII 631 GCTGAGC GC^T[ACGT]AGC
3127 GCTGAGC
DraIII 1961 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3024 CACAACGTG
DrdI 1097 GACGACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2522 GACCTGCCTGTC
EagI 132 CGGCCG C^GGCCG
672 CGGCCG
EclXI 132 CGGCCG C^GGCCG
672 CGGCCG
Eco52I 132 CGGCCG C^GGCCG
672 CGGCCG
HincII 1667 GTCAAC GT[CT]^[GA]AC
2951 GTTAAC
HindII 1667 GTCAAC GT[CT]^[GA]AC
2951 GTTAAC
KasI 170 GGCGCC G^GCGCC
2441 GGCGCC
MaeI 1979 CTAG C^TAG
2887 CTAG
MflI 678 AGATCT [GA]^GATC[CT]
1587 AGATCC
NarI 170 GGCGCC GG^CGCC
2441 GGCGCC
NspI 1929 ACATGC [GA]CATG^[CT]
2760 ACATGC
PflMI 1960 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2014 CCAGGACCTGG
PmaCI 1738 CACGTG CAC^GTG
2837 CACGTG
PmlI 1738 CACGTG CAC^GTG
2837 CACGTG
SfoI 170 GGCGCC GGC^GCC
2441 GGCGCC
SmaI 3099 CCCGGG CCC^GGG
3249 CCCGGG
Tsp509I 1811 AATT ^AATT
2266 AATT
Van91I 1960 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2014 CCAGGACCTGG
XcmI 252 CCACCTGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
426 CCAGTGGACAAGTGG
XhoII 678 AGATCT [GA]^GATC[CT]
1587 AGATCC
XmaCI 3099 CCCGGG C^CCGGG
3249 CCCGGG
XmaI 3099 CCCGGG C^CCGGG
3249 CCCGGG
XmnI 241 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2842 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AhdI AluI AlwI AocI Aor51HI
ApaI AspEI AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco47III Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI Tth111I
TthHB8I