##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AflII AgeI Aor51HI ApaI
AscI AviII AvrII BalI BamHI
BfrI BlnI BsaBI BsiWI BspLU11I
Bst1107I BstXI BstZ17I DraIII DrdI
Eco47III FspI HpaI MamI MluI
MluNI MscI NheI NruI PacI
PaeR7I PinAI PmeI PvuI SbfI
SexAI SfiI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 5419 GGTACC G^GTACC
AclI 2219 AACGTT AA^CGTT
Alw44I 1935 GTGCAC G^TGCAC
ApaLI 1935 GTGCAC G^TGCAC
Asp718I 5419 GGTACC G^GTACC
AspI 4777 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1932 CACGTG CAC^GTG
BcgI 1274 GCAGGAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3366 GGATAC GTATCC(6/5)
BclI 772 TGATCA T^GATCA
BglII 3551 AGATCT A^GATCT
BsaAI 1932 CACGTG [CT]AC^GT[GA]
BsmBI 410 CGTCTC CGTCTC(1/5)
BsmI 364 GCATTC GAATGC(1/-1)
BspDI 1011 ATCGAT AT^CGAT
BssHII 607 GCGCGC G^CGCGC
BstEII 974 GGTGACC G^GT[ACGT]ACC
BstPI 974 GGTGACC G^GT[ACGT]ACC
ClaI 1011 ATCGAT AT^CGAT
CpoI 1079 CGGTCCG CG^G[AT]CCG
EcoO65I 974 GGTGACC G^GT[ACGT]ACC
EcoT22I 4888 ATGCAT ATGCA^T
FbaI 772 TGATCA T^GATCA
FseI 312 GGCCGGCC GGCCGG^CC
HincII 3115 GTCGAC GT[CT]^[GA]AC
HindII 3115 GTCGAC GT[CT]^[GA]AC
KpnI 5419 GGTACC GGTAC^C
NcoI 2779 CCATGG C^CATGG
NotI 1 GCGGCCGC GC^GGCCGC
NsiI 4888 ATGCAT ATGCA^T
PflFI 4777 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1932 CACGTG CAC^GTG
PmlI 1932 CACGTG CAC^GTG
PshAI 3310 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2219 AACGTT AA^CGTT
RsrII 1079 CGGTCCG CG^G[AT]CCG
SalI 3115 GTCGAC G^TCGAC
ScaI 5656 AGTACT AGT^ACT
SpeI 660 ACTAGT A^CTAGT
SspI 3765 AATATT AAT^ATT
Tth111I 4777 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5071 TCTAGA T^CTAGA
XcmI 1609 CCAGCGAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 1931 ACACGT A^C[GA][CT]GT
2269 ACACGT
AhdI 792 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3489 GACATGATGTC
AocI 1898 CCTGAGG CC^T[ACGT]AGG
2678 CCTCAGG
AseI 4532 ATTAAT AT^TAAT
5899 ATTAAT
AsnI 4532 ATTAAT AT^TAAT
5899 ATTAAT
AspEI 792 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3489 GACATGATGTC
BglI 117 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
308 GCCCGGCCGGC
BsiEI 2 CGGCCG CG[GA][CT]^CG
311 CGGCCG
Bsp1407I 2404 TGTACA T^GTACA
4460 TGTACA
BspHI 3536 TCATGA T^CATGA
4554 TCATGA
BsrBI 222 CCGCTC CCGCTC(-3/-3)
1239 GAGCGG
BsrDI 2608 GCAATG GCAATG(2/0)
2715 GCAATG
BsrGI 2404 TGTACA T^GTACA
4460 TGTACA
BstAPI 1721 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4267 GCAGAAAGTGC
Bsu36I 1898 CCTGAGG CC^T[ACGT]AGG
2678 CCTCAGG
DraI 1491 TTTAAA TTT^AAA
4916 TTTAAA
EagI 2 CGGCCG C^GGCCG
311 CGGCCG
Eam1105I 792 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3489 GACATGATGTC
EclXI 2 CGGCCG C^GGCCG
311 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
311 CGGCCG
Eco81I 1898 CCTGAGG CC^T[ACGT]AGG
2678 CCTCAGG
EcoRI 2504 GAATTC G^AATTC
5123 GAATTC
EcoRV 1497 GATATC GAT^ATC
4767 GATATC
MfeI 4175 CAATTG C^AATTG
5302 CAATTG
MunI 4175 CAATTG C^AATTG
5302 CAATTG
NaeI 313 GCCGGC GCC^GGC
575 GCCGGC
NdeI 4431 CATATG CA^TATG
4601 CATATG
NgoMIV 313 GCCGGC G^CCGGC
575 GCCGGC
PflMI 1563 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4031 CCAGGCCTTGG
PshBI 4532 ATTAAT AT^TAAT
5899 ATTAAT
RcaI 3536 TCATGA T^CATGA
4554 TCATGA
SacI 2360 GAGCTC GAGCT^C
4367 GAGCTC
SgrAI 574 CGCCGGCG C[GA]^CCGG[CT]G
1304 CACCGGCG
SmaI 429 CCCGGG CCC^GGG
1125 CCCGGG
SspBI 2404 TGTACA T^GTACA
4460 TGTACA
Van91I 1563 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4031 CCAGGCCTTGG
XmaCI 429 CCCGGG C^CCGGG
1125 CCCGGG
XmaI 429 CCCGGG C^CCGGG
1125 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrFI
BsrI BssKI BssSI BstBI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI NspV PleI PpuMI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SfuI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI