##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
BamHI BbrPI BbvCI BcgI BciVI
BfrI BsaAI BsaBI BsiWI BsmBI
BspDI BspHI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI DraI DrdI
Eam1105I Eco81I EcoRI EcoRV FseI
HindIII HpaI KpnI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflIII 1721 ACATGT A^C[GA][CT]GT
AlwI 2441 GGATC GGATC(4/5)
Aor51HI 662 AGCGCT AGC^GCT
ApaI 763 GGGCCC GGGCC^C
Asp700I 561 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2844 TGCGCA TGC^GCA
AvrII 378 CCTAGG C^CTAGG
BalI 1064 TGGCCA TGG^CCA
BbeI 1149 GGCGCC GGCGC^C
BclI 1523 TGATCA T^GATCA
BglII 1109 AGATCT A^GATCT
BlnI 378 CCTAGG C^CTAGG
BlpI 646 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 646 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 1733 TGTACA T^GTACA
BspLU11I 1721 ACATGT A^CATGT
BsrFI 1493 ACCGGC [GA]^CCGG[CT]
BsrGI 1733 TGTACA T^GTACA
BstEII 2304 GGTGACC G^GT[ACGT]ACC
BstPI 2304 GGTGACC G^GT[ACGT]ACC
BstYI 1109 AGATCT [GA]^GATC[CT]
CelII 646 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1493 ACCGGC [GA]^CCGG[CT]
Eco47III 662 AGCGCT AGC^GCT
EcoO65I 2304 GGTGACC G^GT[ACGT]ACC
EcoT22I 3218 ATGCAT ATGCA^T
FbaI 1523 TGATCA T^GATCA
FspI 2844 TGCGCA TGC^GCA
HincII 1947 GTTGAC GT[CT]^[GA]AC
HindII 1947 GTTGAC GT[CT]^[GA]AC
KasI 1149 GGCGCC G^GCGCC
MflI 1109 AGATCT [GA]^GATC[CT]
MluNI 1064 TGGCCA TGG^CCA
MscI 1064 TGGCCA TGG^CCA
MseI 931 TTAA T^TAA
NarI 1149 GGCGCC GG^CGCC
NdeI 3032 CATATG CA^TATG
NotI 1 GCGGCCGC GC^GGCCGC
NsiI 3218 ATGCAT ATGCA^T
SapI 2235 GCTCTTC GCTCTTC(1/4)
SbfI 1869 CCTGCAGG CCTGCA^GG
SfoI 1149 GGCGCC GGC^GCC
SmaI 83 CCCGGG CCC^GGG
SphI 1953 GCATGC GCATG^C
Sse8387I 1869 CCTGCAGG CCTGCA^GG
SspBI 1733 TGTACA T^GTACA
TaqI 438 TCGA T^CGA
Tru9I 931 TTAA T^TAA
TthHB8I 438 TCGA T^CGA
XhoII 1109 AGATCT [GA]^GATC[CT]
XmaCI 83 CCCGGG C^CCGGG
XmaI 83 CCCGGG C^CCGGG
XmnI 561 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 841 GTCTAC GT^[AC][GT]AC
2433 GTAGAC
AccIII 1535 TCCGGA T^CCGGA
2036 TCCGGA
AcsI 1694 AAATTT [GA]^AATT[CT]
1728 GAATTT
ApoI 1694 AAATTT [GA]^AATT[CT]
1728 GAATTT
BsaWI 1535 TCCGGA [AT]^CCGG[AT]
2036 TCCGGA
BseAI 1535 TCCGGA T^CCGGA
2036 TCCGGA
BsgI 350 CTGCAC GTGCAG(16/14)
1358 CTGCAC
BsmI 1818 GAATGC GAATGC(1/-1)
434 GCATTC
BspEI 1535 TCCGGA T^CCGGA
2036 TCCGGA
BsrBI 13 GAGCGG CCGCTC(-3/-3)
88 GAGCGG
BsrDI 2127 CATTGC GCAATG(2/0)
875 GCAATG
BssSI 1020 CACGAG CACGAG(-5/-1)
2293 CACGAG
BstAPI 1035 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2199 GCAGCAACTGC
CpoI 1537 CGGACCG CG^G[AT]CCG
2270 CGGTCCG
HaeII 662 AGCGCT [GA]GCGC^[CT]
1149 GGCGCC
KspI 5 CCGCGG CCGC^GG
77 CCGCGG
MaeII 143 ACGT A^CGT
1486 ACGT
MroI 1535 TCCGGA T^CCGGA
2036 TCCGGA
PflMI 1157 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2109 CCAGACGCTGG
RsrII 1537 CGGACCG CG^G[AT]CCG
2270 CGGTCCG
SacI 1261 GAGCTC GAGCT^C
2286 GAGCTC
SacII 5 CCGCGG CCGC^GG
77 CCGCGG
SexAI 1313 ACCTGGT A^CC[AT]GGT
2078 ACCAGGT
SmlI 1168 CTTGAG C^T[CT][GA]AG
1332 CTCAAG
StuI 428 AGGCCT AGG^CCT
2798 AGGCCT
Van91I 1157 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2109 CCAGACGCTGG
XcmI 501 CCAGTTCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3001 CCACTGTTTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
Tsp509I TspRI