##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AgeI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BciVI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsaI RsrII SacII
SalI ScaI SexAI SfuI SgrAI
SnaBI SspBI SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1657 GACGTC GACGT^C
AflII 1073 CTTAAG C^TTAAG
AflIII 3280 ACATGT A^C[GA][CT]GT
Alw44I 3276 GTGCAC G^TGCAC
Aor51HI 117 AGCGCT AGC^GCT
ApaLI 3276 GTGCAC G^TGCAC
AvrII 2761 CCTAGG C^CTAGG
BbeI 286 GGCGCC GGCGC^C
BbrPI 1826 CACGTG CAC^GTG
BbsI 11 GAAGAC GAAGAC(2/6)
BfrI 1073 CTTAAG C^TTAAG
BglII 2854 AGATCT A^GATCT
BlnI 2761 CCTAGG C^CTAGG
BlpI 823 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 823 GCTGAGC GC^T[ACGT]AGC
BpuAI 11 GAAGAC GAAGAC(2/6)
BsaAI 1826 CACGTG [CT]AC^GT[GA]
BsaBI 1444 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1223 GAGACC GGTCTC(1/5)
BspLU11I 3280 ACATGT A^CATGT
BsrDI 523 CATTGC GCAATG(2/0)
BstXI 2314 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 823 GCTGAGC GC^T[ACGT]AGC
DraIII 2479 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 117 AGCGCT AGC^GCT
EcoRV 2706 GATATC GAT^ATC
KasI 286 GGCGCC G^GCGCC
MamI 1444 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2580 GCCGGC GCC^GGC
NarI 286 GGCGCC GG^CGCC
NcoI 2394 CCATGG C^CATGG
NgoMIV 2580 GCCGGC G^CCGGC
NheI 1864 GCTAGC G^CTAGC
PmaCI 1826 CACGTG CAC^GTG
PmlI 1826 CACGTG CAC^GTG
SapI 1760 GCTCTTC GCTCTTC(1/4)
SfoI 286 GGCGCC GGC^GCC
SpeI 1672 ACTAGT A^CTAGT
SphI 1926 GCATGC GCATG^C
TaqI 237 TCGA T^CGA
Tsp509I 2647 AATT ^AATT
TthHB8I 237 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 2736 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2834 GACCCAGAGTC
AocI 2193 CCTGAGG CC^T[ACGT]AGG
2384 CCTCAGG
AspEI 2736 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2834 GACCCAGAGTC
BbvCI 2562 CCTCAGC CCTCAGC(-5/-2)
701 GCTGAGG
BcgI 157 CGAGCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
756 CGACAACCGTGC
BclI 1449 TGATCA T^GATCA
1611 TGATCA
BsmI 773 GAATGC GAATGC(1/-1)
1046 GAATGC
BspHI 1107 TCATGA T^CATGA
1653 TCATGA
BsrBI 21 CCGCTC CCGCTC(-3/-3)
451 CCGCTC
BssSI 1097 CTCGTG CACGAG(-5/-1)
1589 CTCGTG
BstAPI 1063 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1999 GCAGGAGCTGC
BstEII 1006 GGTGACC G^GT[ACGT]ACC
1594 GGTCACC
BstPI 1006 GGTGACC G^GT[ACGT]ACC
1594 GGTCACC
Bsu36I 2193 CCTGAGG CC^T[ACGT]AGG
2384 CCTCAGG
DraI 2643 TTTAAA TTT^AAA
2859 TTTAAA
DsaI 2394 CCATGG C^C[GA][CT]GG
3059 CCGTGG
Eam1105I 2736 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2834 GACCCAGAGTC
EarI 1761 CTCTTC CTCTTC(1/4)
1808 CTCTTC
Eco81I 2193 CCTGAGG CC^T[ACGT]AGG
2384 CCTCAGG
EcoO65I 1006 GGTGACC G^GT[ACGT]ACC
1594 GGTCACC
FbaI 1449 TGATCA T^GATCA
1611 TGATCA
HaeII 117 AGCGCT [GA]GCGC^[CT]
286 GGCGCC
Ksp632I 1761 CTCTTC CTCTTC(1/4)
1808 CTCTTC
PleI 3094 GACTC GAGTC(4/5)
2840 GAGTC
PpuMI 429 GGGACCC [GA]G^G[AT]CC[CT]
2832 GGGACCC
RcaI 1107 TCATGA T^CATGA
1653 TCATGA
SbfI 1903 CCTGCAGG CCTGCA^GG
2512 CCTGCAGG
SmlI 1073 CTTAAG C^T[CT][GA]AG
2358 CTCAAG
Sse8387I 1903 CCTGCAGG CCTGCA^GG
2512 CCTGCAGG
StuI 1932 AGGCCT AGG^CCT
2783 AGGCCT
XcmI 1361 CCAGGGCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3050 CCACTGCCACCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PstI PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmaI StyI TfiI Tru9I
TseI Tsp45I TspRI Van91I XhoII
XmaCI XmaI