##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AclI AgeI AhdI Aor51HI AscI
AseI AsnI AspEI BamHI BcgI
BsiWI BssHII CpoI Eam1105I Eco47III
EcoRI EcoRV FseI MfeI MluI
MunI NheI NotI NruI PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SapI SfiI SgrAI
SnaBI StuI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1961 GACGTC GACGT^C
Acc65I 579 GGTACC G^GTACC
AccIII 435 TCCGGA T^CCGGA
AocI 1591 CCTGAGG CC^T[ACGT]AGG
Asp700I 1744 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 579 GGTACC G^GTACC
AspI 5480 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5862 TGCGCA TGC^GCA
AvrII 5174 CCTAGG C^CTAGG
BbeI 5298 GGCGCC GGCGC^C
BbrPI 5994 CACGTG CAC^GTG
BclI 1033 TGATCA T^GATCA
BlnI 5174 CCTAGG C^CTAGG
BseAI 435 TCCGGA T^CCGGA
BspDI 2600 ATCGAT AT^CGAT
BspEI 435 TCCGGA T^CCGGA
BsrBI 1477 CCGCTC CCGCTC(-3/-3)
Bst1107I 1076 GTATAC GTA^TAC
BstBI 681 TTCGAA TT^CGAA
BstZ17I 1076 GTATAC GTA^TAC
Bsu36I 1591 CCTGAGG CC^T[ACGT]AGG
ClaI 2600 ATCGAT AT^CGAT
Eco81I 1591 CCTGAGG CC^T[ACGT]AGG
EcoT22I 4071 ATGCAT ATGCA^T
FbaI 1033 TGATCA T^GATCA
FspI 5862 TGCGCA TGC^GCA
KasI 5298 GGCGCC G^GCGCC
KpnI 579 GGTACC GGTAC^C
KspI 1720 CCGCGG CCGC^GG
MroI 435 TCCGGA T^CCGGA
NarI 5298 GGCGCC GG^CGCC
NcoI 5500 CCATGG C^CATGG
NsiI 4071 ATGCAT ATGCA^T
NspV 681 TTCGAA TT^CGAA
PaeR7I 1736 CTCGAG C^TCGAG
PflFI 5480 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5994 CACGTG CAC^GTG
PmlI 5994 CACGTG CAC^GTG
SacII 1720 CCGCGG CCGC^GG
SalI 3608 GTCGAC G^TCGAC
SbfI 4789 CCTGCAGG CCTGCA^GG
SexAI 3504 ACCTGGT A^CC[AT]GGT
SfoI 5298 GGCGCC GGC^GCC
SfuI 681 TTCGAA TT^CGAA
SmaI 701 CCCGGG CCC^GGG
SpeI 5795 ACTAGT A^CTAGT
Sse8387I 4789 CCTGCAGG CCTGCA^GG
SspI 6612 AATATT AAT^ATT
SwaI 7058 ATTTAAAT ATTT^AAAT
Tth111I 5480 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4341 TCTAGA T^CTAGA
XcmI 5120 CCATCCCATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1736 CTCGAG C^TCGAG
XmaCI 701 CCCGGG C^CCGGG
XmaI 701 CCCGGG C^CCGGG
XmnI 1744 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 5867 ACATGT A^C[GA][CT]GT
6320 ACATGT
ApaI 916 GGGCCC GGGCC^C
2760 GGGCCC
BalI 1491 TGGCCA TGG^CCA
2457 TGGCCA
BciVI 115 GTATCC GTATCC(6/5)
2311 GTATCC
BglII 1215 AGATCT A^GATCT
4123 AGATCT
BlpI 2551 GCTCAGC GC^T[ACGT]AGC
3000 GCTCAGC
Bpu1102I 2551 GCTCAGC GC^T[ACGT]AGC
3000 GCTCAGC
BsaBI 2383 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4939 GATTGACATC
BsaWI 435 TCCGGA [AT]^CCGG[AT]
5234 TCCGGT
BsmBI 3309 CGTCTC CGTCTC(1/5)
5209 GAGACG
BspHI 444 TCATGA T^CATGA
2532 TCATGA
BspLU11I 5867 ACATGT A^CATGT
6320 ACATGT
BstAPI 2834 GCAGTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7021 GCACAGTATGC
BstEII 1675 GGTGACC G^GT[ACGT]ACC
1711 GGTGACC
BstPI 1675 GGTGACC G^GT[ACGT]ACC
1711 GGTGACC
BstXI 5107 CCACGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5913 CCAGCTGCTTGG
CelII 2551 GCTCAGC GC^T[ACGT]AGC
3000 GCTCAGC
DrdI 350 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2279 GACAAAGATGTC
EagI 1854 CGGCCG C^GGCCG
2345 CGGCCG
EclXI 1854 CGGCCG C^GGCCG
2345 CGGCCG
Eco52I 1854 CGGCCG C^GGCCG
2345 CGGCCG
EcoNI 1749 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1906 CCTGCTTCAGG
EcoO65I 1675 GGTGACC G^GT[ACGT]ACC
1711 GGTGACC
HindIII 4306 AAGCTT A^AGCTT
7161 AAGCTT
HpaI 3445 GTTAAC GTT^AAC
6905 GTTAAC
MamI 2383 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4939 GATTGACATC
MluNI 1491 TGGCCA TGG^CCA
2457 TGGCCA
MscI 1491 TGGCCA TGG^CCA
2457 TGGCCA
RcaI 444 TCATGA T^CATGA
2532 TCATGA
ScaI 1812 AGTACT AGT^ACT
4424 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI Alw44I AlwI
AlwNI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII