##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AflIII AgeI AhdI
AscI Asp700I Asp718I AspEI AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BglI BglII BlnI BsaAI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
Eam1105I EcoRV FbaI FseI HindIII
KasI KpnI MluI MroI NaeI
NarI NdeI NgoMIV NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI Sse8387I SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 512 CGCG CG^CG
AclI 1804 AACGTT AA^CGTT
AflII 2590 CTTAAG C^TTAAG
Alw44I 942 GTGCAC G^TGCAC
Aor51HI 2594 AGCGCT AGC^GCT
ApaLI 942 GTGCAC G^TGCAC
AseI 2424 ATTAAT AT^TAAT
AsnI 2424 ATTAAT AT^TAAT
BcgI 2513 CGATACACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2590 CTTAAG C^TTAAG
BsaBI 3834 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 634 GAGACC GGTCTC(1/5)
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 3134 CGTCTC CGTCTC(1/5)
BsrBI 347 CCGCTC CCGCTC(-3/-3)
BstUI 512 CGCG CG^CG
BstXI 1865 CCAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 4002 GGATCT [GA]^GATC[CT]
DraIII 2019 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco47III 2594 AGCGCT AGC^GCT
Eco52I 2 CGGCCG C^GGCCG
EcoNI 3976 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HpaI 3384 GTTAAC GTT^AAC
KspI 511 CCGCGG CCGC^GG
MamI 3834 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 4002 GGATCT [GA]^GATC[CT]
MvnI 512 CGCG CG^CG
NheI 3879 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
PaeR7I 585 CTCGAG C^TCGAG
PflMI 3240 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2424 ATTAAT AT^TAAT
Psp1406I 1804 AACGTT AA^CGTT
SacI 430 GAGCTC GAGCT^C
SacII 511 CCGCGG CCGC^GG
SapI 1792 GAAGAGC GCTCTTC(1/4)
SgrAI 935 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 3523 ACTAGT A^CTAGT
SspI 3439 AATATT AAT^ATT
Van91I 3240 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 585 CTCGAG C^TCGAG
XhoII 4002 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2800 GACGTC GACGT^C
3132 GACGTC
AspI 178 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3200 GACAGTGTC
AviII 2110 TGCGCA TGC^GCA
2748 TGCGCA
BsgI 1193 CTGCAC GTGCAG(16/14)
1433 CTGCAC
BsmI 2575 GCATTC GAATGC(1/-1)
4097 GCATTC
Bsp1407I 2356 TGTACA T^GTACA
3373 TGTACA
BsrFI 321 GCCGGT [GA]^CCGG[CT]
936 GCCGGT
BsrGI 2356 TGTACA T^GTACA
3373 TGTACA
BssSI 3295 CACGAG CACGAG(-5/-1)
163 CTCGTG
BstEII 157 GGTGACC G^GT[ACGT]ACC
2528 GGTCACC
BstPI 157 GGTGACC G^GT[ACGT]ACC
2528 GGTCACC
Cfr10I 321 GCCGGT [GA]^CCGG[CT]
936 GCCGGT
DraI 3023 TTTAAA TTT^AAA
3351 TTTAAA
EcoO65I 157 GGTGACC G^GT[ACGT]ACC
2528 GGTCACC
EcoRI 874 GAATTC G^AATTC
2465 GAATTC
EcoT22I 1885 ATGCAT ATGCA^T
3419 ATGCAT
FspI 2110 TGCGCA TGC^GCA
2748 TGCGCA
HaeII 742 AGCGCC [GA]GCGC^[CT]
2594 AGCGCT
HincII 184 GTCAAC GT[CT]^[GA]AC
3384 GTTAAC
HindII 184 GTCAAC GT[CT]^[GA]AC
3384 GTTAAC
MfeI 2835 CAATTG C^AATTG
3377 CAATTG
MunI 2835 CAATTG C^AATTG
3377 CAATTG
NcoI 1715 CCATGG C^CATGG
2028 CCATGG
NsiI 1885 ATGCAT ATGCA^T
3419 ATGCAT
PflFI 178 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3200 GACAGTGTC
SphI 930 GCATGC GCATG^C
1887 GCATGC
SspBI 2356 TGTACA T^GTACA
3373 TGTACA
TaqI 586 TCGA T^CGA
2069 TCGA
Tth111I 178 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3200 GACAGTGTC
TthHB8I 586 TCGA T^CGA
2069 TCGA
XcmI 197 CCATGACCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3240 CCAAACTCTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmaCI XmaI