##################################
# NO CUT ENZYMES ( 28 enzymes) #
##################################
Acc65I AclI AgeI AhdI AscI
AseI AsnI Asp718I AspEI BssHII
CpoI Eam1105I HpaI KpnI NheI
NotI PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SexAI
SgrAI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AccI 7656 GTATAC GT^[AC][GT]AC
AccIII 8498 TCCGGA T^CCGGA
AvrII 10490 CCTAGG C^CTAGG
BcgI 223 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4233 GTATCC GTATCC(6/5)
BlnI 10490 CCTAGG C^CTAGG
BseAI 8498 TCCGGA T^CCGGA
BsiWI 657 CGTACG C^GTACG
BspDI 7369 ATCGAT AT^CGAT
BspEI 8498 TCCGGA T^CCGGA
Bst1107I 7656 GTATAC GTA^TAC
BstBI 4025 TTCGAA TT^CGAA
BstZ17I 7656 GTATAC GTA^TAC
ClaI 7369 ATCGAT AT^CGAT
DraI 10741 TTTAAA TTT^AAA
DrdI 10022 GACACTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 8553 GGCCGGCC GGCCGG^CC
MroI 8498 TCCGGA T^CCGGA
NdeI 7925 CATATG CA^TATG
NruI 2863 TCGCGA TCG^CGA
NspV 4025 TTCGAA TT^CGAA
SapI 3195 GAAGAGC GCTCTTC(1/4)
ScaI 8945 AGTACT AGT^ACT
SfuI 4025 TTCGAA TT^CGAA
SnaBI 5517 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3349 GACGTC GACGT^C
8170 GACGTC
BbrPI 421 CACGTG CAC^GTG
9543 CACGTG
BbvCI 5606 CCTCAGC CCTCAGC(-5/-2)
2772 GCTGAGG
BclI 8930 TGATCA T^GATCA
11111 TGATCA
BglII 4700 AGATCT A^GATCT
6056 AGATCT
BsaWI 3058 ACCGGA [AT]^CCGG[AT]
8498 TCCGGA
BsmI 7743 GAATGC GAATGC(1/-1)
2153 GCATTC
BspLU11I 736 ACATGT A^CATGT
4244 ACATGT
BssSI 240 CACGAG CACGAG(-5/-1)
9408 CTCGTG
EcoT22I 7365 ATGCAT ATGCA^T
10438 ATGCAT
FbaI 8930 TGATCA T^GATCA
11111 TGATCA
HindIII 8935 AAGCTT A^AGCTT
10814 AAGCTT
KspI 4954 CCGCGG CCGC^GG
7511 CCGCGG
MluI 4 ACGCGT A^CGCGT
4961 ACGCGT
NsiI 7365 ATGCAT ATGCA^T
10438 ATGCAT
PmaCI 421 CACGTG CAC^GTG
9543 CACGTG
PmlI 421 CACGTG CAC^GTG
9543 CACGTG
PshAI 696 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5202 GACATCCGTC
SacII 4954 CCGCGG CCGC^GG
7511 CCGCGG
SbfI 5214 CCTGCAGG CCTGCA^GG
8247 CCTGCAGG
SfiI 987 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
8544 GGCCGTGCGGGCC
SmaI 2957 CCCGGG CCC^GGG
4792 CCCGGG
Sse8387I 5214 CCTGCAGG CCTGCA^GG
8247 CCTGCAGG
SspI 10278 AATATT AAT^ATT
10287 AATATT
XmaCI 2957 CCCGGG C^CCGGG
4792 CCCGGG
XmaI 2957 CCCGGG C^CCGGG
4792 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 179 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI Asp700I AspHI AspI AvaI
AvaII AviII BalI BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BfrI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PaeR7I PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI