##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BlnI BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eam1105I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KpnI MamI MluI
MluNI MroI MscI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 3156 GTCTAC GT^[AC][GT]AC
AclI 1868 AACGTT AA^CGTT
AflII 3313 CTTAAG C^TTAAG
AflIII 2474 ACACGT A^C[GA][CT]GT
AocI 92 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2940 AGCGCT AGC^GCT
AseI 1615 ATTAAT AT^TAAT
AsnI 1615 ATTAAT AT^TAAT
AspI 991 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 245 CCCGGG C^[CT]CG[GA]G
BbeI 210 GGCGCC GGCGC^C
BbsI 1414 GAAGAC GAAGAC(2/6)
BcgI 261 CGAGTTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3313 CTTAAG C^TTAAG
BpuAI 1414 GAAGAC GAAGAC(2/6)
BsaI 1450 GAGACC GGTCTC(1/5)
BsoBI 245 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3047 TGTACA T^GTACA
BspHI 2343 TCATGA T^CATGA
BspMI 2762 ACCTGC ACCTGC(4/8)
BsrDI 2111 GCAATG GCAATG(2/0)
BsrFI 200 GCCGGC [GA]^CCGG[CT]
BsrGI 3047 TGTACA T^GTACA
BstAPI 2757 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2912 CCATGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 92 CCTGAGG CC^T[ACGT]AGG
Cfr10I 200 GCCGGC [GA]^CCGG[CT]
DraIII 806 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2940 AGCGCT AGC^GCT
Eco81I 92 CCTGAGG CC^T[ACGT]AGG
EcoRI 712 GAATTC G^AATTC
KasI 210 GGCGCC G^GCGCC
NaeI 200 GCCGGC GCC^GGC
NarI 210 GGCGCC GG^CGCC
NgoMIV 200 GCCGGC G^CCGGC
PflFI 991 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1977 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1615 ATTAAT AT^TAAT
Psp1406I 1868 AACGTT AA^CGTT
RcaI 2343 TCATGA T^CATGA
SacI 2284 GAGCTC GAGCT^C
SfoI 210 GGCGCC GGC^GCC
SmaI 245 CCCGGG CCC^GGG
SspBI 3047 TGTACA T^GTACA
Tth111I 991 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 913 TCTAGA T^CTAGA
XmaCI 245 CCCGGG C^CCGGG
XmaI 245 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BanI 210 GGCGCC G^G[CT][GA]CC
2638 GGCACC
BanII 2284 GAGCTC G[GA]GC[CT]^C
2687 GAGCCC
BciVI 549 GGATAC GTATCC(6/5)
1443 GTATCC
BclI 1986 TGATCA T^GATCA
2967 TGATCA
BlpI 2936 GCTGAGC GC^T[ACGT]AGC
2943 GCTGAGC
Bpu1102I 2936 GCTGAGC GC^T[ACGT]AGC
2943 GCTGAGC
BsmI 888 GAATGC GAATGC(1/-1)
569 GCATTC
BssHII 353 GCGCGC G^CGCGC
382 GCGCGC
CelII 2936 GCTGAGC GC^T[ACGT]AGC
2943 GCTGAGC
DraI 1297 TTTAAA TTT^AAA
3227 TTTAAA
DraII 320 AGGGCCT [GA]G^G[ACGT]CC[CT]
1977 AGGACCT
EaeI 129 CGGCCG [CT]^GGCC[GA]
309 CGGCCG
EagI 129 CGGCCG C^GGCCG
309 CGGCCG
EarI 937 GAAGAG CTCTTC(1/4)
1054 GAAGAG
EclXI 129 CGGCCG C^GGCCG
309 CGGCCG
Eco52I 129 CGGCCG C^GGCCG
309 CGGCCG
EcoO109I 320 AGGGCCT [GA]G^G[ACGT]CC[CT]
1977 AGGACCT
FbaI 1986 TGATCA T^GATCA
2967 TGATCA
HgaI 144 GACGC GACGC(5/10)
189 GCGTC
HindIII 537 AAGCTT A^AGCTT
2188 AAGCTT
Ksp632I 937 GAAGAG CTCTTC(1/4)
1054 GAAGAG
MaeII 1869 ACGT A^CGT
2476 ACGT
MfeI 48 CAATTG C^AATTG
2165 CAATTG
MunI 48 CAATTG C^AATTG
2165 CAATTG
NspI 494 ACATGC [GA]CATG^[CT]
2609 GCATGT
PflMI 1030 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCAGATCTTGG
Van91I 1030 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCAGATCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI