##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI Aor51HI AscI AseI AsnI
Asp700I AviII BamHI BbvCI BclI
BglII BsmI BstBI BstYI DraIII
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII MfeI MflI
MunI NdeI NheI NotI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI SalI SapI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1249 GGTACC G^GTACC
AccIII 1887 TCCGGA T^CCGGA
AcsI 6412 GAATTT [GA]^AATT[CT]
AflII 4582 CTTAAG C^TTAAG
AgeI 2746 ACCGGT A^CCGGT
Alw44I 524 GTGCAC G^TGCAC
ApaLI 524 GTGCAC G^TGCAC
ApoI 6412 GAATTT [GA]^AATT[CT]
Asp718I 1249 GGTACC G^GTACC
AspI 625 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6575 CCTAGG C^CTAGG
BbrPI 4657 CACGTG CAC^GTG
BcgI 2619 GCAGGCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4582 CTTAAG C^TTAAG
BlnI 6575 CCTAGG C^CTAGG
BlpI 1929 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1929 GCTCAGC GC^T[ACGT]AGC
BseAI 1887 TCCGGA T^CCGGA
BsiWI 4511 CGTACG C^GTACG
BspDI 1297 ATCGAT AT^CGAT
BspEI 1887 TCCGGA T^CCGGA
BspHI 45 TCATGA T^CATGA
BssHII 2271 GCGCGC G^CGCGC
Bst1107I 6681 GTATAC GTA^TAC
BstZ17I 6681 GTATAC GTA^TAC
CelII 1929 GCTCAGC GC^T[ACGT]AGC
ClaI 1297 ATCGAT AT^CGAT
CpoI 6615 CGGTCCG CG^G[AT]CCG
DraI 4526 TTTAAA TTT^AAA
KpnI 1249 GGTACC GGTAC^C
KspI 423 CCGCGG CCGC^GG
MluI 1458 ACGCGT A^CGCGT
MroI 1887 TCCGGA T^CCGGA
NruI 2077 TCGCGA TCG^CGA
PflFI 625 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2746 ACCGGT A^CCGGT
PmaCI 4657 CACGTG CAC^GTG
PmlI 4657 CACGTG CAC^GTG
RcaI 45 TCATGA T^CATGA
RsrII 6615 CGGTCCG CG^G[AT]CCG
SacII 423 CCGCGG CCGC^GG
SbfI 6216 CCTGCAGG CCTGCA^GG
ScaI 129 AGTACT AGT^ACT
SexAI 4342 ACCTGGT A^CC[AT]GGT
SfiI 1483 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2544 GCATGC GCATG^C
Sse8387I 6216 CCTGCAGG CCTGCA^GG
Tth111I 625 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 4724 GTCTAC GT^[AC][GT]AC
6681 GTATAC
BbsI 622 GAAGAC GAAGAC(2/6)
4190 GTCTTC
BciVI 1652 GGATAC GTATCC(6/5)
6310 GTATCC
BpuAI 622 GAAGAC GAAGAC(2/6)
4190 GTCTTC
BsaAI 4513 TACGTG [CT]AC^GT[GA]
4657 CACGTG
BsaBI 1300 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1306 GATCGACATC
BsiEI 1966 CGGCCG CG[GA][CT]^CG
1990 CGGCCG
BspLU11I 4006 ACATGT A^CATGT
5724 ACATGT
BssSI 1215 CTCGTG CACGAG(-5/-1)
6173 CTCGTG
BstEII 2692 GGTTACC G^GT[ACGT]ACC
2742 GGTCACC
BstPI 2692 GGTTACC G^GT[ACGT]ACC
2742 GGTCACC
DrdI 1696 GACAGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5839 GACACCTGGGTC
EagI 1966 CGGCCG C^GGCCG
1990 CGGCCG
EclXI 1966 CGGCCG C^GGCCG
1990 CGGCCG
Eco52I 1966 CGGCCG C^GGCCG
1990 CGGCCG
EcoO65I 2692 GGTTACC G^GT[ACGT]ACC
2742 GGTCACC
HincII 547 GTTAAC GT[CT]^[GA]AC
4294 GTTAAC
HindII 547 GTTAAC GT[CT]^[GA]AC
4294 GTTAAC
HpaI 547 GTTAAC GTT^AAC
4294 GTTAAC
MamI 1300 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1306 GATCGACATC
SacI 1414 GAGCTC GAGCT^C
3074 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI AspEI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI