##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI Asp718I AspEI AviII
AvrII BamHI BlnI BlpI Bpu1102I
BseAI BsiWI BspDI BspEI BspMI
BssHII BssSI BstAPI BstEII BstPI
CelII ClaI DraIII Eam1105I EcoNI
EcoO65I FseI FspI KpnI MluI
MroI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI SbfI SexAI SgrAI SnaBI
SphI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 5730 CTTAAG C^TTAAG
Aor51HI 2859 AGCGCT AGC^GCT
BalI 6128 TGGCCA TGG^CCA
BbrPI 1156 CACGTG CAC^GTG
BbsI 722 GAAGAC GAAGAC(2/6)
BciVI 1324 GGATAC GTATCC(6/5)
BfrI 5730 CTTAAG C^TTAAG
BpuAI 722 GAAGAC GAAGAC(2/6)
BsaAI 1156 CACGTG [CT]AC^GT[GA]
BsaBI 938 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1213 ACCGGA [AT]^CCGG[AT]
BsmI 5355 GAATGC GAATGC(1/-1)
Bsp1407I 948 TGTACA T^GTACA
BspLU11I 4302 ACATGT A^CATGT
BsrBI 224 CCGCTC CCGCTC(-3/-3)
BsrGI 948 TGTACA T^GTACA
Bst1107I 1801 GTATAC GTA^TAC
BstZ17I 1801 GTATAC GTA^TAC
DrdI 4581 GACGGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2859 AGCGCT AGC^GCT
EcoRI 1384 GAATTC G^AATTC
EcoRV 5167 GATATC GAT^ATC
EcoT22I 5782 ATGCAT ATGCA^T
HgaI 206 GACGC GACGC(5/10)
KspI 103 CCGCGG CCGC^GG
MamI 938 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 6128 TGGCCA TGG^CCA
MscI 6128 TGGCCA TGG^CCA
NsiI 5782 ATGCAT ATGCA^T
PflMI 2950 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1156 CACGTG CAC^GTG
PmlI 1156 CACGTG CAC^GTG
PpuMI 409 GGGACCT [GA]G^G[AT]CC[CT]
SacII 103 CCGCGG CCGC^GG
SalI 4590 GTCGAC G^TCGAC
SapI 4457 GAAGAGC GCTCTTC(1/4)
ScaI 5103 AGTACT AGT^ACT
SpeI 3389 ACTAGT A^CTAGT
SspBI 948 TGTACA T^GTACA
StuI 54 AGGCCT AGG^CCT
Van91I 2950 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2332 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 1157 ACGTGT A^C[GA][CT]GT
4302 ACATGT
Alw44I 4908 GTGCAC G^TGCAC
5464 GTGCAC
AocI 1229 CCTGAGG CC^T[ACGT]AGG
6192 CCTGAGG
ApaLI 4908 GTGCAC G^TGCAC
5464 GTGCAC
AseI 2375 ATTAAT AT^TAAT
5159 ATTAAT
AsnI 2375 ATTAAT AT^TAAT
5159 ATTAAT
Asp700I 1043 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5005 GAATGGATTC
AspI 206 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6399 GACTCCGTC
BanI 116 GGCGCC G^G[CT][GA]CC
488 GGCGCC
BbeI 116 GGCGCC GGCGC^C
488 GGCGCC
BbvCI 160 GCTGAGG CCTCAGC(-5/-2)
3878 GCTGAGG
BglII 2254 AGATCT A^GATCT
2613 AGATCT
BsgI 6371 CTGCAC GTGCAG(16/14)
770 GTGCAG
BstBI 2997 TTCGAA TT^CGAA
3756 TTCGAA
BstYI 2254 AGATCT [GA]^GATC[CT]
2613 AGATCT
Bsu36I 1229 CCTGAGG CC^T[ACGT]AGG
6192 CCTGAGG
CpoI 94 CGGTCCG CG^G[AT]CCG
172 CGGACCG
Eco81I 1229 CCTGAGG CC^T[ACGT]AGG
6192 CCTGAGG
HindIII 815 AAGCTT A^AGCTT
1656 AAGCTT
HpaI 2051 GTTAAC GTT^AAC
2903 GTTAAC
KasI 116 GGCGCC G^GCGCC
488 GGCGCC
MfeI 3243 CAATTG C^AATTG
3354 CAATTG
MflI 2254 AGATCT [GA]^GATC[CT]
2613 AGATCT
MunI 3243 CAATTG C^AATTG
3354 CAATTG
NarI 116 GGCGCC GG^CGCC
488 GGCGCC
NdeI 3653 CATATG CA^TATG
4364 CATATG
NspV 2997 TTCGAA TT^CGAA
3756 TTCGAA
PflFI 206 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6399 GACTCCGTC
PshBI 2375 ATTAAT AT^TAAT
5159 ATTAAT
RsrII 94 CGGTCCG CG^G[AT]CCG
172 CGGACCG
SacI 2783 GAGCTC GAGCT^C
3035 GAGCTC
SfiI 40 GGCCGTAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
267 GGCCGGGCGGGCC
SfoI 116 GGCGCC GGC^GCC
488 GGCGCC
SfuI 2997 TTCGAA TT^CGAA
3756 TTCGAA
SmaI 72 CCCGGG CCC^GGG
349 CCCGGG
SwaI 5795 ATTTAAAT ATTT^AAAT
6112 ATTTAAAT
Tth111I 206 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6399 GACTCCGTC
XhoII 2254 AGATCT [GA]^GATC[CT]
2613 AGATCT
XmaCI 72 CCCGGG C^CCGGG
349 CCCGGG
XmaI 72 CCCGGG C^CCGGG
349 CCCGGG
XmnI 1043 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5005 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanII
BbvI BcgI BclI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI