##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AccIII AclI AhdI AlwNI
Aor51HI AscI AspEI AspI AvrII
BbrPI BcgI BclI BlnI BseAI
BsiWI BspEI CpoI DrdI Eam1105I
Eco47III EcoRV FbaI FseI HindIII
MfeI MluI MroI MunI NdeI
NheI NotI NruI PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SexAI
SgrAI SnaBI SwaI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1944 GGTACC G^GTACC
AcyI 19 GGCGCC G[GA]^CG[CT]C
AgeI 3632 ACCGGT A^CCGGT
Alw44I 3059 GTGCAC G^TGCAC
ApaLI 3059 GTGCAC G^TGCAC
Asp718I 1944 GGTACC G^GTACC
AviII 3301 TGCGCA TGC^GCA
BalI 5286 TGGCCA TGG^CCA
BamHI 1026 GGATCC G^GATCC
BbeI 19 GGCGCC GGCGC^C
BciVI 4170 GGATAC GTATCC(6/5)
BlpI 3122 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3122 GCTGAGC GC^T[ACGT]AGC
BsaAI 2874 TACGTG [CT]AC^GT[GA]
BsaBI 5316 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 19 GGCGCC G[GA]^CG[CT]C
BsgI 864 CTGCAC GTGCAG(16/14)
BsmBI 2946 GAGACG CGTCTC(1/5)
BsmI 4743 GAATGC GAATGC(1/-1)
BspDI 3328 ATCGAT AT^CGAT
BspHI 5710 TCATGA T^CATGA
BssSI 540 CTCGTG CACGAG(-5/-1)
Bst1107I 4289 GTATAC GTA^TAC
BstAPI 2631 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2731 TTCGAA TT^CGAA
BstEII 4590 GGTGACC G^GT[ACGT]ACC
BstPI 4590 GGTGACC G^GT[ACGT]ACC
BstZ17I 4289 GTATAC GTA^TAC
CelII 3122 GCTGAGC GC^T[ACGT]AGC
ClaI 3328 ATCGAT AT^CGAT
EcoO65I 4590 GGTGACC G^GT[ACGT]ACC
FspI 3301 TGCGCA TGC^GCA
HgaI 2948 GACGC GACGC(5/10)
Hin1I 19 GGCGCC G[GA]^CG[CT]C
HpaI 4754 GTTAAC GTT^AAC
KasI 19 GGCGCC G^GCGCC
KpnI 1944 GGTACC GGTAC^C
MamI 5316 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5286 TGGCCA TGG^CCA
MscI 5286 TGGCCA TGG^CCA
NaeI 257 GCCGGC GCC^GGC
NarI 19 GGCGCC GG^CGCC
NgoMIV 257 GCCGGC G^CCGGC
NspV 2731 TTCGAA TT^CGAA
PinAI 3632 ACCGGT A^CCGGT
RcaI 5710 TCATGA T^CATGA
SacI 4913 GAGCTC GAGCT^C
SalI 2548 GTCGAC G^TCGAC
SapI 2833 GCTCTTC GCTCTTC(1/4)
SbfI 2637 CCTGCAGG CCTGCA^GG
ScaI 1518 AGTACT AGT^ACT
SfiI 112 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 19 GGCGCC GGC^GCC
SfuI 2731 TTCGAA TT^CGAA
SpeI 1521 ACTAGT A^CTAGT
Sse8387I 2637 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2548 GTCGAC GT^[AC][GT]AC
4289 GTATAC
AflII 1163 CTTAAG C^TTAAG
4106 CTTAAG
AseI 621 ATTAAT AT^TAAT
4925 ATTAAT
AsnI 621 ATTAAT AT^TAAT
4925 ATTAAT
Asp700I 3326 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3411 GAAATGGTTC
BbvCI 2187 CCTCAGC CCTCAGC(-5/-2)
3997 GCTGAGG
BfrI 1163 CTTAAG C^TTAAG
4106 CTTAAG
BsaWI 344 TCCGGT [AT]^CCGG[AT]
3632 ACCGGT
BsrDI 2430 CATTGC GCAATG(2/0)
763 GCAATG
BsrFI 257 GCCGGC [GA]^CCGG[CT]
3632 ACCGGT
Cfr10I 257 GCCGGC [GA]^CCGG[CT]
3632 ACCGGT
EcoRI 1638 GAATTC G^AATTC
4309 GAATTC
EcoT22I 4075 ATGCAT ATGCA^T
5107 ATGCAT
HincII 2548 GTCGAC GT[CT]^[GA]AC
4754 GTTAAC
HindII 2548 GTCGAC GT[CT]^[GA]AC
4754 GTTAAC
NcoI 499 CCATGG C^CATGG
1828 CCATGG
NsiI 4075 ATGCAT ATGCA^T
5107 ATGCAT
PshBI 621 ATTAAT AT^TAAT
4925 ATTAAT
StuI 1917 AGGCCT AGG^CCT
3500 AGGCCT
XmnI 3326 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3411 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrBI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI