##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccII AccIII AgeI AhdI
AocI AscI Asp718I AspEI AviII
BbeI BglI BlpI Bpu1102I BseAI
BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BstEII BstPI BstUI Bsu36I
CelII Cfr10I CpoI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I FseI
FspI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PinAI
PmeI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1847 GACGTC GACGT^C
AclI 9916 AACGTT AA^CGTT
ApaI 5118 GGGCCC GGGCC^C
AvrII 8578 CCTAGG C^CTAGG
BanI 7293 GGTGCC G^G[CT][GA]CC
BbrPI 7808 CACGTG CAC^GTG
BbvCI 8888 CCTCAGC CCTCAGC(-5/-2)
BlnI 8578 CCTAGG C^CTAGG
BspDI 764 ATCGAT AT^CGAT
BspMI 9618 GCAGGT ACCTGC(4/8)
Bst1107I 1026 GTATAC GTA^TAC
BstBI 6976 TTCGAA TT^CGAA
BstZ17I 1026 GTATAC GTA^TAC
ClaI 764 ATCGAT AT^CGAT
DrdI 8256 GACAAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1511 GATATC GAT^ATC
HgaI 6364 GACGC GACGC(5/10)
HpaI 345 GTTAAC GTT^AAC
NspV 6976 TTCGAA TT^CGAA
PaeR7I 7640 CTCGAG C^TCGAG
PflMI 8048 CCAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 7808 CACGTG CAC^GTG
PmlI 7808 CACGTG CAC^GTG
PshAI 2793 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 9916 AACGTT AA^CGTT
SapI 10339 GCTCTTC GCTCTTC(1/4)
SexAI 9123 ACCTGGT A^CC[AT]GGT
SfuI 6976 TTCGAA TT^CGAA
SphI 4207 GCATGC GCATG^C
StuI 3483 AGGCCT AGG^CCT
Van91I 8048 CCAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 7640 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1847 GACGTC G[GA]^CG[CT]C
6364 GACGCC
AflII 1221 CTTAAG C^TTAAG
5750 CTTAAG
Alw44I 5977 GTGCAC G^TGCAC
10473 GTGCAC
Aor51HI 16 AGCGCT AGC^GCT
8861 AGCGCT
ApaLI 5977 GTGCAC G^TGCAC
10473 GTGCAC
AspI 1314 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6094 GACTGGGTC
BcgI 6252 CGAGCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1015 GCAGCCAGATCG
BfrI 1221 CTTAAG C^TTAAG
5750 CTTAAG
BsaHI 1847 GACGTC G[GA]^CG[CT]C
6364 GACGCC
BsaWI 39 TCCGGT [AT]^CCGG[AT]
983 ACCGGA
BsmBI 162 CGTCTC CGTCTC(1/5)
4825 CGTCTC
BspHI 6007 TCATGA T^CATGA
7556 TCATGA
BstAPI 1529 GCATTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
9998 GCATATTTTGC
DraIII 4598 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
9292 CACGCAGTG
Eco47III 16 AGCGCT AGC^GCT
8861 AGCGCT
HaeII 16 AGCGCT [GA]GCGC^[CT]
8861 AGCGCT
Hin1I 1847 GACGTC G[GA]^CG[CT]C
6364 GACGCC
NcoI 715 CCATGG C^CATGG
10358 CCATGG
PflFI 1314 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6094 GACTGGGTC
PstI 6659 CTGCAG CTGCA^G
7365 CTGCAG
RcaI 6007 TCATGA T^CATGA
7556 TCATGA
SacI 6253 GAGCTC GAGCT^C
7842 GAGCTC
ScaI 2364 AGTACT AGT^ACT
4767 AGTACT
Tth111I 1314 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6094 GACTGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BalI BamHI BanII BbsI BbvI
BciVI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI