##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
ApaI AscI Asp700I AspI AviII
BbeI BglII BsaI BsaWI BseAI
BsiEI BsmBI BsmI BspEI BsrFI
BssHII BstAPI BstBI Cfr10I CpoI
DraIII EagI EclXI Eco47III Eco52I
FseI FspI HaeII HpaI KasI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 1088 GACGCC G[GA]^CG[CT]C
AflII 3729 CTTAAG C^TTAAG
AhdI 2678 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1882 ATTAAT AT^TAAT
AsnI 1882 ATTAAT AT^TAAT
AspEI 2678 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3213 CTCGAG C^[CT]CG[GA]G
AvrII 5219 CCTAGG C^CTAGG
BalI 3037 TGGCCA TGG^CCA
BamHI 3227 GGATCC G^GATCC
BbrPI 547 CACGTG CAC^GTG
BbvCI 1112 CCTCAGC CCTCAGC(-5/-2)
BcnI 2143 CCCGG CC^[GC]GG
BfrI 3729 CTTAAG C^TTAAG
BglI 1696 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5219 CCTAGG C^CTAGG
BsaBI 5718 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1088 GACGCC G[GA]^CG[CT]C
BsiWI 1813 CGTACG C^GTACG
BsoBI 3213 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4424 TGTACA T^GTACA
BspDI 183 ATCGAT AT^CGAT
BspHI 4159 TCATGA T^CATGA
BspLU11I 4646 ACATGT A^CATGT
BsrBI 5385 CCGCTC CCGCTC(-3/-3)
BsrGI 4424 TGTACA T^GTACA
Bst1107I 4581 GTATAC GTA^TAC
BstZ17I 4581 GTATAC GTA^TAC
ClaI 183 ATCGAT AT^CGAT
Eam1105I 2678 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4820 GAATTC G^AATTC
EcoT22I 1427 ATGCAT ATGCA^T
HapII 2144 CCGG C^CGG
Hin1I 1088 GACGCC G[GA]^CG[CT]C
HindIII 4285 AAGCTT A^AGCTT
HpaII 2144 CCGG C^CGG
MamI 5718 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3037 TGGCCA TGG^CCA
MscI 3037 TGGCCA TGG^CCA
MspI 2144 CCGG C^CGG
NciI 2143 CCCGG CC^[GC]GG
NsiI 1427 ATGCAT ATGCA^T
PaeR7I 3213 CTCGAG C^TCGAG
PflMI 5165 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 547 CACGTG CAC^GTG
PmlI 547 CACGTG CAC^GTG
PpuMI 2684 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1882 ATTAAT AT^TAAT
RcaI 4159 TCATGA T^CATGA
ScaI 3209 AGTACT AGT^ACT
SpeI 3458 ACTAGT A^CTAGT
SspBI 4424 TGTACA T^GTACA
StuI 2066 AGGCCT AGG^CCT
Van91I 5165 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 404 CCACAGTTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3213 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1309 GGTACC G^GTACC
5311 GGTACC
AccII 32 CGCG CG^CG
599 CGCG
Alw44I 9 GTGCAC G^TGCAC
4254 GTGCAC
AocI 1513 CCTTAGG CC^T[ACGT]AGG
2680 CCTCAGG
ApaLI 9 GTGCAC G^TGCAC
4254 GTGCAC
Asp718I 1309 GGTACC G^GTACC
5311 GGTACC
BcgI 880 CGAAGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1066 CGACTCCCCTGC
BclI 5236 TGATCA T^GATCA
5594 TGATCA
BssSI 1103 CACGAG CACGAG(-5/-1)
4251 CTCGTG
BstEII 589 GGTGACC G^GT[ACGT]ACC
3142 GGTGACC
BstPI 589 GGTGACC G^GT[ACGT]ACC
3142 GGTGACC
BstUI 32 CGCG CG^CG
599 CGCG
Bsu36I 1513 CCTTAGG CC^T[ACGT]AGG
2680 CCTCAGG
DraII 2684 AGGTCCC [GA]G^G[ACGT]CC[CT]
4124 AGGCCCT
Eco81I 1513 CCTTAGG CC^T[ACGT]AGG
2680 CCTCAGG
EcoO109I 2684 AGGTCCC [GA]G^G[ACGT]CC[CT]
4124 AGGCCCT
EcoO65I 589 GGTGACC G^GT[ACGT]ACC
3142 GGTGACC
EcoRV 1992 GATATC GAT^ATC
5702 GATATC
FbaI 5236 TGATCA T^GATCA
5594 TGATCA
HgaI 1088 GACGC GACGC(5/10)
600 GCGTC
HincII 3504 GTTGAC GT[CT]^[GA]AC
4045 GTTGAC
HindII 3504 GTTGAC GT[CT]^[GA]AC
4045 GTTGAC
KpnI 1309 GGTACC GGTAC^C
5311 GGTACC
MfeI 5622 CAATTG C^AATTG
5649 CAATTG
MunI 5622 CAATTG C^AATTG
5649 CAATTG
MvnI 32 CGCG CG^CG
599 CGCG
NcoI 858 CCATGG C^CATGG
3694 CCATGG
SacI 2206 GAGCTC GAGCT^C
2717 GAGCTC
SexAI 2428 ACCAGGT A^CC[AT]GGT
5600 ACCAGGT
SphI 1162 GCATGC GCATG^C
5107 GCATGC
XbaI 826 TCTAGA T^CTAGA
4663 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BciVI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DrdI DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII