##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII BbeI BbrPI
BcgI BglI BlpI Bpu1102I BsiEI
BsiWI BspDI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI FspI HpaI
KasI KpnI KspI MluI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SapI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 194 TCCGGA T^CCGGA
AcyI 1961 GACGCC G[GA]^CG[CT]C
AflII 4673 CTTAAG C^TTAAG
Alw44I 3918 GTGCAC G^TGCAC
ApaLI 3918 GTGCAC G^TGCAC
AvrII 2113 CCTAGG C^CTAGG
BamHI 2512 GGATCC G^GATCC
BfrI 4673 CTTAAG C^TTAAG
BglII 3572 AGATCT A^GATCT
BlnI 2113 CCTAGG C^CTAGG
BsaAI 4890 CACGTA [CT]AC^GT[GA]
BsaBI 1929 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1961 GACGCC G[GA]^CG[CT]C
BsaWI 194 TCCGGA [AT]^CCGG[AT]
BseAI 194 TCCGGA T^CCGGA
BsmBI 778 GAGACG CGTCTC(1/5)
Bsp1407I 5589 TGTACA T^GTACA
BspEI 194 TCCGGA T^CCGGA
BsrFI 1467 GCCGGT [GA]^CCGG[CT]
BsrGI 5589 TGTACA T^GTACA
BstBI 2727 TTCGAA TT^CGAA
Cfr10I 1467 GCCGGT [GA]^CCGG[CT]
DraIII 1194 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 876 GAATTC G^AATTC
FauI 1266 GCGGG CCCGC(4/6)
Hin1I 1961 GACGCC G[GA]^CG[CT]C
MamI 1929 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 194 TCCGGA T^CCGGA
NcoI 2757 CCATGG C^CATGG
NdeI 1938 CATATG CA^TATG
NspV 2727 TTCGAA TT^CGAA
PaeR7I 3138 CTCGAG C^TCGAG
PpuMI 1055 AGGTCCT [GA]G^G[AT]CC[CT]
SalI 1830 GTCGAC G^TCGAC
ScaI 5417 AGTACT AGT^ACT
SfuI 2727 TTCGAA TT^CGAA
SspBI 5589 TGTACA T^GTACA
StuI 2107 AGGCCT AGG^CCT
SwaI 4445 ATTTAAAT ATTT^AAAT
XbaI 1418 TCTAGA T^CTAGA
XhoI 3138 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1108 GTCTAC GT^[AC][GT]AC
1830 GTCGAC
AflIII 4247 ACATGT A^C[GA][CT]GT
4514 ACATGT
AseI 3671 ATTAAT AT^TAAT
4798 ATTAAT
AsnI 3671 ATTAAT AT^TAAT
4798 ATTAAT
Asp700I 1944 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5474 GAAAAGTTTC
AvaI 2559 CTCGGG C^[CT]CG[GA]G
3138 CTCGAG
BalI 2499 TGGCCA TGG^CCA
3600 TGGCCA
BciVI 1318 GGATAC GTATCC(6/5)
5249 GGATAC
BclI 604 TGATCA T^GATCA
2437 TGATCA
BcnI 1360 CCGGG CC^[GC]GG
1678 CCGGG
BsaI 1476 GGTCTC GGTCTC(1/5)
3999 GGTCTC
BsoBI 2559 CTCGGG C^[CT]CG[GA]G
3138 CTCGAG
BspHI 880 TCATGA T^CATGA
3525 TCATGA
BspLU11I 4247 ACATGT A^CATGT
4514 ACATGT
BsrDI 3099 CATTGC GCAATG(2/0)
2739 GCAATG
DraII 1055 AGGTCCT [GA]G^G[ACGT]CC[CT]
1793 GGGGCCT
DsaI 1580 CCACGG C^C[GA][CT]GG
2757 CCATGG
EcoO109I 1055 AGGTCCT [GA]G^G[ACGT]CC[CT]
1793 GGGGCCT
EcoT22I 1946 ATGCAT ATGCA^T
1974 ATGCAT
FbaI 604 TGATCA T^GATCA
2437 TGATCA
HaeII 611 GGCGCT [GA]GCGC^[CT]
1269 GGCGCT
HincII 1830 GTCGAC GT[CT]^[GA]AC
3870 GTCAAC
HindII 1830 GTCGAC GT[CT]^[GA]AC
3870 GTCAAC
MfeI 748 CAATTG C^AATTG
2086 CAATTG
MluNI 2499 TGGCCA TGG^CCA
3600 TGGCCA
MscI 2499 TGGCCA TGG^CCA
3600 TGGCCA
MspA1I 156 CAGCTG C[AC]G^C[GT]G
4485 CAGCTG
MunI 748 CAATTG C^AATTG
2086 CAATTG
NciI 1360 CCGGG CC^[GC]GG
1678 CCGGG
NsiI 1946 ATGCAT ATGCA^T
1974 ATGCAT
PshBI 3671 ATTAAT AT^TAAT
4798 ATTAAT
PvuII 156 CAGCTG CAG^CTG
4485 CAGCTG
RcaI 880 TCATGA T^CATGA
3525 TCATGA
SacI 73 GAGCTC GAGCT^C
1519 GAGCTC
SexAI 3995 ACCAGGT A^CC[AT]GGT
4951 ACCTGGT
XmnI 1944 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5474 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EarI Eco81I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PshAI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII