##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI AscI Asp718I AspI
AvrII BbeI BbrPI BbvCI BcgI
BclI BglII BlnI BsaHI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII BssSI BstEII
BstPI ClaI CpoI DraIII DrdI
Eco47III EcoO65I EcoRV FbaI HgaI
Hin1I KasI KpnI KspI MluI
MroI NarI NdeI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SmaI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 944 CGCG CG^CG
AhdI 4878 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1298 GTGCAC G^TGCAC
ApaLI 1298 GTGCAC G^TGCAC
AseI 3882 ATTAAT AT^TAAT
AsnI 3882 ATTAAT AT^TAAT
Asp700I 3666 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4878 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 35 TGCGCA TGC^GCA
BamHI 737 GGATCC G^GATCC
BciVI 1427 GGATAC GTATCC(6/5)
Bst1107I 1174 GTATAC GTA^TAC
BstBI 367 TTCGAA TT^CGAA
BstUI 944 CGCG CG^CG
BstZ17I 1174 GTATAC GTA^TAC
Eam1105I 4878 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5056 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 44 GGCCGGCC GGCCGG^CC
FspI 35 TGCGCA TGC^GCA
HaeII 52 GGCGCT [GA]GCGC^[CT]
HindIII 5044 AAGCTT A^AGCTT
HpaI 3564 GTTAAC GTT^AAC
MvnI 944 CGCG CG^CG
NcoI 189 CCATGG C^CATGG
NheI 208 GCTAGC G^CTAGC
NspV 367 TTCGAA TT^CGAA
PflMI 2184 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1305 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 3882 ATTAAT AT^TAAT
SacI 936 GAGCTC GAGCT^C
SfuI 367 TTCGAA TT^CGAA
SphI 1261 GCATGC GCATG^C
StuI 846 AGGCCT AGG^CCT
Van91I 2184 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3666 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1174 GTATAC GT^[AC][GT]AC
5233 GTCTAC
AflII 4464 CTTAAG C^TTAAG
4771 CTTAAG
AocI 849 CCTCAGG CC^T[ACGT]AGG
3147 CCTTAGG
AvaI 1404 CCCGAG C^[CT]CG[GA]G
5175 CCCGAG
BalI 297 TGGCCA TGG^CCA
4986 TGGCCA
BbsI 1399 GTCTTC GAAGAC(2/6)
1664 GTCTTC
BfrI 4464 CTTAAG C^TTAAG
4771 CTTAAG
BglI 1343 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4684 GCCCTGTTGGC
BpuAI 1399 GTCTTC GAAGAC(2/6)
1664 GTCTTC
BsaAI 1177 TACGTA [CT]AC^GT[GA]
2103 TACGTA
BsaBI 503 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1737 GATGCCCATC
BsaI 3005 GAGACC GGTCTC(1/5)
854 GGTCTC
BsiEI 43 CGGCCG CG[GA][CT]^CG
47 CGGCCG
BsoBI 1404 CCCGAG C^[CT]CG[GA]G
5175 CCCGAG
Bsp1407I 2737 TGTACA T^GTACA
5325 TGTACA
BsrGI 2737 TGTACA T^GTACA
5325 TGTACA
BstAPI 127 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2986 GCATTGCCTGC
BstXI 229 CCACTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2334 CCATTAAGGTGG
Bsu36I 849 CCTCAGG CC^T[ACGT]AGG
3147 CCTTAGG
DsaI 189 CCATGG C^C[GA][CT]GG
3170 CCGTGG
EagI 43 CGGCCG C^GGCCG
47 CGGCCG
EclXI 43 CGGCCG C^GGCCG
47 CGGCCG
Eco52I 43 CGGCCG C^GGCCG
47 CGGCCG
Eco81I 849 CCTCAGG CC^T[ACGT]AGG
3147 CCTTAGG
MaeII 1178 ACGT A^CGT
2104 ACGT
MamI 503 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1737 GATGCCCATC
MluNI 297 TGGCCA TGG^CCA
4986 TGGCCA
MscI 297 TGGCCA TGG^CCA
4986 TGGCCA
PvuII 4733 CAGCTG CAG^CTG
4938 CAGCTG
SexAI 1302 ACCAGGT A^CC[AT]GGT
4034 ACCAGGT
SnaBI 1177 TACGTA TAC^GTA
2103 TACGTA
SpeI 4236 ACTAGT A^CTAGT
5114 ACTAGT
SspBI 2737 TGTACA T^GTACA
5325 TGTACA
XbaI 412 TCTAGA T^CTAGA
5487 TCTAGA
XcmI 1384 CCAATCCTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3858 CCATAAAGGATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII