##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AspEI AspI BclI BsiWI
BsmBI BspDI BssHII BssSI BstBI
ClaI CpoI DsaI Eam1105I Eco47III
FbaI FseI HpaI KspI MluI
NcoI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SapI SbfI
SfiI SfuI SgrAI SpeI Sse8387I
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 5894 GGTACC G^GTACC
AccII 2028 CGCG CG^CG
AccIII 2009 TCCGGA T^CCGGA
AflII 4332 CTTAAG C^TTAAG
Asp718I 5894 GGTACC G^GTACC
AviII 290 TGCGCA TGC^GCA
AvrII 4457 CCTAGG C^CTAGG
BbeI 1706 GGCGCC GGCGC^C
BbsI 3445 GAAGAC GAAGAC(2/6)
BfrI 4332 CTTAAG C^TTAAG
BlnI 4457 CCTAGG C^CTAGG
BpuAI 3445 GAAGAC GAAGAC(2/6)
BsaBI 4886 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2009 TCCGGA [AT]^CCGG[AT]
BseAI 2009 TCCGGA T^CCGGA
BsiEI 952 CGGCCG CG[GA][CT]^CG
BsmI 2937 GCATTC GAATGC(1/-1)
BspEI 2009 TCCGGA T^CCGGA
BspLU11I 3700 ACATGT A^CATGT
BstAPI 4474 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1233 GGTGACC G^GT[ACGT]ACC
BstPI 1233 GGTGACC G^GT[ACGT]ACC
BstUI 2028 CGCG CG^CG
DrdI 4073 GACAAATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 952 CGGCCG C^GGCCG
EclXI 952 CGGCCG C^GGCCG
Eco52I 952 CGGCCG C^GGCCG
EcoNI 474 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1233 GGTGACC G^GT[ACGT]ACC
EcoRV 3249 GATATC GAT^ATC
FspI 290 TGCGCA TGC^GCA
KasI 1706 GGCGCC G^GCGCC
KpnI 5894 GGTACC GGTAC^C
MamI 4886 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2009 TCCGGA T^CCGGA
MvnI 2028 CGCG CG^CG
NarI 1706 GGCGCC GG^CGCC
NdeI 5710 CATATG CA^TATG
SacI 199 GAGCTC GAGCT^C
SalI 643 GTCGAC G^TCGAC
ScaI 3878 AGTACT AGT^ACT
SfoI 1706 GGCGCC GGC^GCC
SmaI 1142 CCCGGG CCC^GGG
SnaBI 4554 TACGTA TAC^GTA
StuI 596 AGGCCT AGG^CCT
XmaCI 1142 CCCGGG C^CCGGG
XmaI 1142 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 2382 ACGTGT A^C[GA][CT]GT
3700 ACATGT
Alw44I 1857 GTGCAC G^TGCAC
2866 GTGCAC
ApaI 1049 GGGCCC GGGCC^C
5628 GGGCCC
ApaLI 1857 GTGCAC G^TGCAC
2866 GTGCAC
AseI 1719 ATTAAT AT^TAAT
3662 ATTAAT
AsnI 1719 ATTAAT AT^TAAT
3662 ATTAAT
Asp700I 1275 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4435 GAAAAAGTTC
BamHI 2006 GGATCC G^GATCC
4891 GGATCC
BlpI 3034 GCTGAGC GC^T[ACGT]AGC
3483 GCTGAGC
Bpu1102I 3034 GCTGAGC GC^T[ACGT]AGC
3483 GCTGAGC
Bsp1407I 752 TGTACA T^GTACA
1493 TGTACA
BspHI 3382 TCATGA T^CATGA
3845 TCATGA
BsrBI 1367 GAGCGG CCGCTC(-3/-3)
2545 GAGCGG
BsrGI 752 TGTACA T^GTACA
1493 TGTACA
Bst1107I 4908 GTATAC GTA^TAC
6080 GTATAC
BstXI 62 CCAGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1586 CCATATTCATGG
BstZ17I 4908 GTATAC GTA^TAC
6080 GTATAC
CelII 3034 GCTGAGC GC^T[ACGT]AGC
3483 GCTGAGC
DraIII 487 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5387 CACTATGTG
EcoRI 766 GAATTC G^AATTC
3101 GAATTC
EcoT22I 4545 ATGCAT ATGCA^T
6118 ATGCAT
HindIII 2375 AAGCTT A^AGCTT
3224 AAGCTT
MfeI 2811 CAATTG C^AATTG
3684 CAATTG
MunI 2811 CAATTG C^AATTG
3684 CAATTG
NaeI 98 GCCGGC GCC^GGC
954 GCCGGC
NgoMIV 98 GCCGGC G^CCGGC
954 GCCGGC
NheI 1311 GCTAGC G^CTAGC
2508 GCTAGC
NsiI 4545 ATGCAT ATGCA^T
6118 ATGCAT
PaeR7I 196 CTCGAG C^TCGAG
2068 CTCGAG
PshBI 1719 ATTAAT AT^TAAT
3662 ATTAAT
RcaI 3382 TCATGA T^CATGA
3845 TCATGA
SspBI 752 TGTACA T^GTACA
1493 TGTACA
SwaI 5029 ATTTAAAT ATTT^AAAT
5343 ATTTAAAT
XhoI 196 CTCGAG C^TCGAG
2068 CTCGAG
XmnI 1275 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4435 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbvCI BbvI BcgI
BciVI BcnI BfaI BglI BglII
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII