##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII Aor51HI AscI
AseI AsnI BfrI BglII BsaBI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
HpaI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2001 GGTACC G^GTACC
AflIII 3004 ACACGT A^C[GA][CT]GT
AgeI 1998 ACCGGT A^CCGGT
AhdI 5022 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2001 GGTACC G^GTACC
AspEI 5022 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4156 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 860 TGCGCA TGC^GCA
BbrPI 914 CACGTG CAC^GTG
BciVI 1324 GGATAC GTATCC(6/5)
BlpI 3796 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3796 GCTCAGC GC^T[ACGT]AGC
BsaAI 914 CACGTG [CT]AC^GT[GA]
BspHI 2925 TCATGA T^CATGA
BsrBI 3728 GAGCGG CCGCTC(-3/-3)
BsrDI 5365 CATTGC GCAATG(2/0)
BssHII 2700 GCGCGC G^CGCGC
BssSI 4422 CACGAG CACGAG(-5/-1)
BstAPI 787 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3796 GCTCAGC GC^T[ACGT]AGC
DraIII 2734 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5022 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 860 TGCGCA TGC^GCA
HindIII 4535 AAGCTT A^AGCTT
KpnI 2001 GGTACC GGTAC^C
KspI 3 CCGCGG CCGC^GG
NdeI 2822 CATATG CA^TATG
PaeR7I 2535 CTCGAG C^TCGAG
PflFI 4156 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1998 ACCGGT A^CCGGT
PmaCI 914 CACGTG CAC^GTG
PmlI 914 CACGTG CAC^GTG
RcaI 2925 TCATGA T^CATGA
SacII 3 CCGCGG CCGC^GG
SalI 4498 GTCGAC G^TCGAC
ScaI 5138 AGTACT AGT^ACT
SexAI 1623 ACCAGGT A^CC[AT]GGT
SphI 4036 GCATGC GCATG^C
SspI 4709 AATATT AAT^ATT
Tth111I 4156 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2535 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 1285 TCCGGA T^CCGGA
2112 TCCGGA
ApaI 117 GGGCCC GGGCC^C
5237 GGGCCC
Asp700I 652 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2881 GAAACCCTTC
AvrII 1081 CCTAGG C^CTAGG
3837 CCTAGG
BamHI 3347 GGATCC G^GATCC
5324 GGATCC
BbeI 211 GGCGCC GGCGC^C
3828 GGCGCC
BcgI 2786 CGATGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2842 CGAAGGACCTGC
BclI 519 TGATCA T^GATCA
4781 TGATCA
BlnI 1081 CCTAGG C^CTAGG
3837 CCTAGG
BseAI 1285 TCCGGA T^CCGGA
2112 TCCGGA
BspEI 1285 TCCGGA T^CCGGA
2112 TCCGGA
BstEII 1453 GGTGACC G^GT[ACGT]ACC
1828 GGTGACC
BstPI 1453 GGTGACC G^GT[ACGT]ACC
1828 GGTGACC
BstXI 4265 CCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4830 CCAGCACTTTGG
DraI 2544 TTTAAA TTT^AAA
5257 TTTAAA
EcoO65I 1453 GGTGACC G^GT[ACGT]ACC
1828 GGTGACC
FbaI 519 TGATCA T^GATCA
4781 TGATCA
HgaI 704 GACGC GACGC(5/10)
256 GCGTC
KasI 211 GGCGCC G^GCGCC
3828 GGCGCC
MroI 1285 TCCGGA T^CCGGA
2112 TCCGGA
NarI 211 GGCGCC GG^CGCC
3828 GGCGCC
NspI 1219 GCATGT [GA]CATG^[CT]
4036 GCATGC
SacI 812 GAGCTC GAGCT^C
3794 GAGCTC
SapI 1669 GAAGAGC GCTCTTC(1/4)
2274 GAAGAGC
SfoI 211 GGCGCC GGC^GCC
3828 GGCGCC
SmaI 1415 CCCGGG CCC^GGG
4089 CCCGGG
StuI 654 AGGCCT AGG^CCT
3231 AGGCCT
XmaCI 1415 CCCGGG C^CCGGG
4089 CCCGGG
XmaI 1415 CCCGGG C^CCGGG
4089 CCCGGG
XmnI 652 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2881 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII