##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp718I BclI
BfrI BsmI BspLU11I BsrDI BssHII
Bst1107I BstBI BstZ17I CpoI DraIII
DrdI Eco47III FbaI FseI HincII
HindII HpaI KpnI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfuI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1145 GACGTC GACGT^C
AccI 1700 GTCTAC GT^[AC][GT]AC
AspI 3693 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1597 TGCGCA TGC^GCA
AvrII 1005 CCTAGG C^CTAGG
BamHI 4031 GGATCC G^GATCC
BbrPI 3905 CACGTG CAC^GTG
BcgI 800 GCAGAAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3464 GGATAC GTATCC(6/5)
BglII 3559 AGATCT A^GATCT
BlnI 1005 CCTAGG C^CTAGG
BsaBI 704 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 156 CGGCCG CG[GA][CT]^CG
BsiWI 3943 CGTACG C^GTACG
BspDI 3075 ATCGAT AT^CGAT
BsrBI 350 GAGCGG CCGCTC(-3/-3)
BssSI 3469 CACGAG CACGAG(-5/-1)
ClaI 3075 ATCGAT AT^CGAT
DraI 4225 TTTAAA TTT^AAA
EagI 156 CGGCCG C^GGCCG
EclXI 156 CGGCCG C^GGCCG
Eco52I 156 CGGCCG C^GGCCG
EcoNI 2597 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3306 GAATTC G^AATTC
EcoRV 2472 GATATC GAT^ATC
EcoT22I 4565 ATGCAT ATGCA^T
FspI 1597 TGCGCA TGC^GCA
MamI 704 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 4565 ATGCAT ATGCA^T
PflFI 3693 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3905 CACGTG CAC^GTG
PmlI 3905 CACGTG CAC^GTG
PshAI 3233 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 3186 AGTACT AGT^ACT
SgrAI 110 CGCCGGCG C[GA]^CCGG[CT]G
Tth111I 3693 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2447 TCCGGA T^CCGGA
2524 TCCGGA
AflIII 3906 ACGTGT A^C[GA][CT]GT
3940 ACACGT
AhdI 1878 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3509 GACGGCCTGTC
Alw44I 576 GTGCAC G^TGCAC
3031 GTGCAC
AocI 1992 CCTGAGG CC^T[ACGT]AGG
3503 CCTGAGG
ApaI 2222 GGGCCC GGGCC^C
3723 GGGCCC
ApaLI 576 GTGCAC G^TGCAC
3031 GTGCAC
Asp700I 1686 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2348 GAACAACTTC
AspEI 1878 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3509 GACGGCCTGTC
BalI 1288 TGGCCA TGG^CCA
1786 TGGCCA
BbvCI 3098 CCTCAGC CCTCAGC(-5/-2)
4062 GCTGAGG
BsaAI 3905 CACGTG [CT]AC^GT[GA]
3941 CACGTA
BseAI 2447 TCCGGA T^CCGGA
2524 TCCGGA
BsmBI 20 CGTCTC CGTCTC(1/5)
623 CGTCTC
Bsp1407I 3295 TGTACA T^GTACA
3571 TGTACA
BspEI 2447 TCCGGA T^CCGGA
2524 TCCGGA
BspHI 848 TCATGA T^CATGA
1553 TCATGA
BsrGI 3295 TGTACA T^GTACA
3571 TGTACA
Bsu36I 1992 CCTGAGG CC^T[ACGT]AGG
3503 CCTGAGG
Eam1105I 1878 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3509 GACGGCCTGTC
Eco81I 1992 CCTGAGG CC^T[ACGT]AGG
3503 CCTGAGG
MluNI 1288 TGGCCA TGG^CCA
1786 TGGCCA
MroI 2447 TCCGGA T^CCGGA
2524 TCCGGA
MscI 1288 TGGCCA TGG^CCA
1786 TGGCCA
NspI 1343 GCATGT [GA]CATG^[CT]
2306 GCATGT
PflMI 1058 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3222 CCAGCTATTGG
RcaI 848 TCATGA T^CATGA
1553 TCATGA
SacI 2685 GAGCTC GAGCT^C
2781 GAGCTC
SapI 824 GAAGAGC GCTCTTC(1/4)
4296 GCTCTTC
SbfI 3861 CCTGCAGG CCTGCA^GG
4550 CCTGCAGG
SmaI 176 CCCGGG CCC^GGG
413 CCCGGG
Sse8387I 3861 CCTGCAGG CCTGCA^GG
4550 CCTGCAGG
SspBI 3295 TGTACA T^GTACA
3571 TGTACA
Van91I 1058 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3222 CCAGCTATTGG
XmaCI 176 CCCGGG C^CCGGG
413 CCCGGG
XmaI 176 CCCGGG C^CCGGG
413 CCCGGG
XmnI 1686 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2348 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII