##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BbvCI BcgI BcnI
BlnI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsmBI BsoBI BspDI BsrFI
BssHII BssSI BstBI BstUI CelII
Cfr10I ClaI CpoI EagI EarI
EclXI Eco47III Eco52I FauI FseI
FspI Hin1I KasI KpnI Ksp632I
KspI MluI MvnI NaeI NarI
NciI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmaI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccIII 14 TCCGGA T^CCGGA
AciI 1542 CCGC CCGC(-3/-1)
AflII 3155 CTTAAG C^TTAAG
AhdI 1030 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4417 CCTTAGG CC^T[ACGT]AGG
ApaI 4446 GGGCCC GGGCC^C
AseI 1954 ATTAAT AT^TAAT
AsnI 1954 ATTAAT AT^TAAT
Asp700I 328 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1030 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3258 GAGCTC G[AT]GC[AT]^C
BbrPI 1327 CACGTG CAC^GTG
BciVI 4531 GTATCC GTATCC(6/5)
BfrI 3155 CTTAAG C^TTAAG
BglI 1064 GCCCCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1835 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1037 GGTCTC GGTCTC(1/5)
BsaWI 14 TCCGGA [AT]^CCGG[AT]
BseAI 14 TCCGGA T^CCGGA
BseRI 1175 CTCCTC GAGGAG(10/8)
BsgI 3780 CTGCAC GTGCAG(16/14)
BsiHKAI 3258 GAGCTC G[AT]GC[AT]^C
BspEI 14 TCCGGA T^CCGGA
BspHI 3944 TCATGA T^CATGA
BspLU11I 4653 ACATGT A^CATGT
BsrBI 1542 CCGCTC CCGCTC(-3/-3)
Bst1107I 2209 GTATAC GTA^TAC
BstAPI 3782 GCACAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1027 GGTGACC G^GT[ACGT]ACC
BstPI 1027 GGTGACC G^GT[ACGT]ACC
BstZ17I 2209 GTATAC GTA^TAC
Bsu36I 4417 CCTTAGG CC^T[ACGT]AGG
DraIII 3783 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2589 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1508 CCATGG C^C[GA][CT]GG
Eam1105I 1030 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4417 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1027 GGTGACC G^GT[ACGT]ACC
EcoRV 2183 GATATC GAT^ATC
HapII 15 CCGG C^CGG
HindIII 1998 AAGCTT A^AGCTT
HpaI 3412 GTTAAC GTT^AAC
HpaII 15 CCGG C^CGG
MamI 1835 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3855 CAATTG C^AATTG
MroI 14 TCCGGA T^CCGGA
MspI 15 CCGG C^CGG
MunI 3855 CAATTG C^AATTG
NcoI 1508 CCATGG C^CATGG
NdeI 2030 CATATG CA^TATG
NheI 3387 GCTAGC G^CTAGC
PmaCI 1327 CACGTG CAC^GTG
PmlI 1327 CACGTG CAC^GTG
PshBI 1954 ATTAAT AT^TAAT
RcaI 3944 TCATGA T^CATGA
SacI 3258 GAGCTC GAGCT^C
SbfI 484 CCTGCAGG CCTGCA^GG
ScaI 2324 AGTACT AGT^ACT
SnaBI 1808 TACGTA TAC^GTA
SpeI 3433 ACTAGT A^CTAGT
SphI 2295 GCATGC GCATG^C
Sse8387I 484 CCTGCAGG CCTGCA^GG
StuI 694 AGGCCT AGG^CCT
XbaI 2423 TCTAGA T^CTAGA
XmnI 328 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 2209 GTATAC GT^[AC][GT]AC
2854 GTCTAC
AflIII 4605 ACGTGT A^C[GA][CT]GT
4653 ACATGT
BanII 3258 GAGCTC G[GA]GC[CT]^C
4446 GGGCCC
BglII 704 AGATCT A^GATCT
4228 AGATCT
BsaAI 1327 CACGTG [CT]AC^GT[GA]
1808 TACGTA
EcoNI 2700 CCTTGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3779 CCTGCACAAGG
HgaI 18 GACGC GACGC(5/10)
7 GCGTC
HincII 236 GTCAAC GT[CT]^[GA]AC
3412 GTTAAC
HindII 236 GTCAAC GT[CT]^[GA]AC
3412 GTTAAC
MspA1I 512 CAGCTG C[AC]G^C[GT]G
985 CAGCTG
PstI 485 CTGCAG CTGCA^G
1346 CTGCAG
PvuII 512 CAGCTG CAG^CTG
985 CAGCTG
XcmI 1508 CCATGGAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4589 CCAGAAGTTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BalI BanI BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII