##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
Aor51HI ApaI AscI AseI AsnI
AspI AviII AvrII BciVI BlnI
BsiWI BspDI Bst1107I BstBI BstZ17I
ClaI CpoI DraIII DrdI Eco47III
EcoRV FseI FspI MfeI MluI
MunI NdeI NheI NotI NruI
NspV PacI PflFI PmeI PshBI
RsrII SalI SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2224 GGTACC G^GTACC
AccIII 2310 TCCGGA T^CCGGA
AclI 2470 AACGTT AA^CGTT
AflII 2596 CTTAAG C^TTAAG
AgeI 3020 ACCGGT A^CCGGT
AocI 162 CCTCAGG CC^T[ACGT]AGG
Asp718I 2224 GGTACC G^GTACC
BamHI 3462 GGATCC G^GATCC
BbeI 1946 GGCGCC GGCGC^C
BcgI 1227 CGATGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2596 CTTAAG C^TTAAG
BglII 3002 AGATCT A^GATCT
BsaBI 3363 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2283 GGTCTC GGTCTC(1/5)
BseAI 2310 TCCGGA T^CCGGA
BspEI 2310 TCCGGA T^CCGGA
BsrDI 4353 CATTGC GCAATG(2/0)
BssSI 1407 CACGAG CACGAG(-5/-1)
Bsu36I 162 CCTCAGG CC^T[ACGT]AGG
DraII 1397 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 162 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1397 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 843 ATGCAT ATGCA^T
HindIII 4864 AAGCTT A^AGCTT
HpaI 4613 GTTAAC GTT^AAC
KasI 1946 GGCGCC G^GCGCC
KpnI 2224 GGTACC GGTAC^C
MamI 3363 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2310 TCCGGA T^CCGGA
NaeI 76 GCCGGC GCC^GGC
NarI 1946 GGCGCC GG^CGCC
NgoMIV 76 GCCGGC G^CCGGC
NsiI 843 ATGCAT ATGCA^T
PinAI 3020 ACCGGT A^CCGGT
PpuMI 1397 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1805 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2470 AACGTT AA^CGTT
PvuI 1095 CGATCG CGAT^CG
SexAI 4012 ACCAGGT A^CC[AT]GGT
SfiI 729 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1946 GGCGCC GGC^GCC
XbaI 311 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 3097 GACGTC GACGT^C
4154 GACGTC
AccI 201 GTCTAC GT^[AC][GT]AC
2464 GTAGAC
Alw44I 3016 GTGCAC G^TGCAC
4792 GTGCAC
ApaLI 3016 GTGCAC G^TGCAC
4792 GTGCAC
BanII 774 GAGCTC G[GA]GC[CT]^C
1540 GAGCTC
BlpI 1542 GCTCAGC GC^T[ACGT]AGC
2985 GCTGAGC
Bpu1102I 1542 GCTCAGC GC^T[ACGT]AGC
2985 GCTGAGC
Bsp1407I 4315 TGTACA T^GTACA
5120 TGTACA
BspLU11I 1151 ACATGT A^CATGT
1427 ACATGT
BsrGI 4315 TGTACA T^GTACA
5120 TGTACA
BssHII 689 GCGCGC G^CGCGC
924 GCGCGC
BstEII 1027 GGTGACC G^GT[ACGT]ACC
2823 GGTGACC
BstPI 1027 GGTGACC G^GT[ACGT]ACC
2823 GGTGACC
BstXI 3452 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3977 CCAGAGGCGTGG
CelII 1542 GCTCAGC GC^T[ACGT]AGC
2985 GCTGAGC
EagI 78 CGGCCG C^GGCCG
2710 CGGCCG
EclXI 78 CGGCCG C^GGCCG
2710 CGGCCG
Eco52I 78 CGGCCG C^GGCCG
2710 CGGCCG
EcoO65I 1027 GGTGACC G^GT[ACGT]ACC
2823 GGTGACC
EcoRI 530 GAATTC G^AATTC
3546 GAATTC
HincII 4180 GTCAAC GT[CT]^[GA]AC
4613 GTTAAC
HindII 4180 GTCAAC GT[CT]^[GA]AC
4613 GTTAAC
PaeR7I 2904 CTCGAG C^TCGAG
3133 CTCGAG
PflMI 1065 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2964 CCAGCGGATGG
SacI 774 GAGCTC GAGCT^C
1540 GAGCTC
SapI 1254 GAAGAGC GCTCTTC(1/4)
3348 GCTCTTC
SmaI 648 CCCGGG CCC^GGG
2318 CCCGGG
SspBI 4315 TGTACA T^GTACA
5120 TGTACA
SspI 4484 AATATT AAT^ATT
5052 AATATT
StuI 1703 AGGCCT AGG^CCT
3476 AGGCCT
Van91I 1065 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2964 CCAGCGGATGG
XcmI 2622 CCACTTCACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3878 CCAGGCTCATGATGG
XhoI 2904 CTCGAG C^TCGAG
3133 CTCGAG
XmaCI 648 CCCGGG C^CCGGG
2318 CCCGGG
XmaI 648 CCCGGG C^CCGGG
2318 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI Asp700I AspEI AspHI AvaI
AvaII BalI BanI BbrPI BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DsaI EaeI
Eam1105I EarI EcoNI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PstI PvuII RcaI
RsaI SacII Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI