##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
ApaI AscI Asp718I AvaI AviII
AvrII BamHI BbrPI BcgI BglI
BglII BlnI BsaAI BsiEI BsiWI
BsmBI BsoBI BspDI BsrBI BssHII
BstBI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HpaI KpnI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SalI ScaI SfiI
SfuI SgrAI SmaI SnaBI SphI
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 3478 GTATAC GT^[AC][GT]AC
AccIII 1032 TCCGGA T^CCGGA
AcyI 2054 GGCGCC G[GA]^CG[CT]C
Alw44I 172 GTGCAC G^TGCAC
ApaLI 172 GTGCAC G^TGCAC
Asp700I 3195 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 637 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2054 GGCGCC G^G[CT][GA]CC
BanII 2470 GGGCTC G[GA]GC[CT]^C
BbeI 2054 GGCGCC GGCGC^C
BbvCI 2082 GCTGAGG CCTCAGC(-5/-2)
BsaBI 4309 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2054 GGCGCC G[GA]^CG[CT]C
BsaWI 1032 TCCGGA [AT]^CCGG[AT]
BseAI 1032 TCCGGA T^CCGGA
BsmI 2609 GAATGC GAATGC(1/-1)
BspEI 1032 TCCGGA T^CCGGA
BsrDI 2653 CATTGC GCAATG(2/0)
BssSI 1155 CTCGTG CACGAG(-5/-1)
Bst1107I 3478 GTATAC GTA^TAC
BstAPI 1151 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2312 GGTTACC G^GT[ACGT]ACC
BstPI 2312 GGTTACC G^GT[ACGT]ACC
BstYI 790 GGATCT [GA]^GATC[CT]
BstZ17I 3478 GTATAC GTA^TAC
DraIII 1804 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2312 GGTTACC G^GT[ACGT]ACC
HaeII 2054 GGCGCC [GA]GCGC^[CT]
HgaI 1018 GCGTC GACGC(5/10)
Hin1I 2054 GGCGCC G[GA]^CG[CT]C
KasI 2054 GGCGCC G^GCGCC
KspI 48 CCGCGG CCGC^GG
MamI 4309 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 790 GGATCT [GA]^GATC[CT]
MroI 1032 TCCGGA T^CCGGA
NarI 2054 GGCGCC GG^CGCC
NdeI 4263 CATATG CA^TATG
PflFI 637 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 48 CCGCGG CCGC^GG
SapI 212 GAAGAGC GCTCTTC(1/4)
SbfI 1540 CCTGCAGG CCTGCA^GG
SfoI 2054 GGCGCC GGC^GCC
SpeI 3260 ACTAGT A^CTAGT
Sse8387I 1540 CCTGCAGG CCTGCA^GG
Tth111I 637 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 790 GGATCT [GA]^GATC[CT]
XmnI 3195 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 3 CGCG CG^CG
49 CGCG
AflII 482 CTTAAG C^TTAAG
3644 CTTAAG
AflIII 1326 ACATGT A^C[GA][CT]GT
4232 ACATGT
AhdI 2505 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2980 GACTGTATGTC
AspEI 2505 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2980 GACTGTATGTC
BalI 1989 TGGCCA TGG^CCA
4197 TGGCCA
BclI 1725 TGATCA T^GATCA
4581 TGATCA
BfrI 482 CTTAAG C^TTAAG
3644 CTTAAG
BlpI 2038 GCTGAGC GC^T[ACGT]AGC
4146 GCTTAGC
Bpu1102I 2038 GCTGAGC GC^T[ACGT]AGC
4146 GCTTAGC
BsaI 4174 GAGACC GGTCTC(1/5)
133 GGTCTC
BsmFI 1738 GGGAC GGGAC(10/14)
142 GTCCC
Bsp1407I 4515 TGTACA T^GTACA
4694 TGTACA
BspLU11I 1326 ACATGT A^CATGT
4232 ACATGT
BsrFI 630 ACCGGC [GA]^CCGG[CT]
2574 GCCGGT
BsrGI 4515 TGTACA T^GTACA
4694 TGTACA
BstUI 3 CGCG CG^CG
49 CGCG
CelII 2038 GCTGAGC GC^T[ACGT]AGC
4146 GCTTAGC
CfoI 2 GCGC GCG^C
2055 GCGC
Cfr10I 630 ACCGGC [GA]^CCGG[CT]
2574 GCCGGT
DrdI 2439 GACAGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2634 GACAGAGAAGTC
Eam1105I 2505 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2980 GACTGTATGTC
EcoRI 320 GAATTC G^AATTC
3361 GAATTC
EcoT14I 54 CCATGG C^C[AT][AT]GG
1551 CCATGG
FbaI 1725 TGATCA T^GATCA
4581 TGATCA
HhaI 2 GCGC GCG^C
2055 GCGC
HinP1I 2 GCGC G^CGC
2055 GCGC
MluNI 1989 TGGCCA TGG^CCA
4197 TGGCCA
MscI 1989 TGGCCA TGG^CCA
4197 TGGCCA
MvnI 3 CGCG CG^CG
49 CGCG
NcoI 54 CCATGG C^CATGG
1551 CCATGG
PflMI 1647 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2658 CCATTTCCTGG
SexAI 1702 ACCAGGT A^CC[AT]GGT
1995 ACCTGGT
SspBI 4515 TGTACA T^GTACA
4694 TGTACA
StyI 54 CCATGG C^C[AT][AT]GG
1551 CCATGG
TaqI 111 TCGA T^CGA
1690 TCGA
TthHB8I 111 TCGA T^CGA
1690 TCGA
Van91I 1647 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2658 CCATTTCCTGG
XbaI 3485 TCTAGA T^CTAGA
4053 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI