##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI Asp718I AspEI AvrII BbeI
BbrPI BcgI BclI BglII BlnI
BsaAI BsaBI BsiWI BspDI BspHI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI Eam1105I EcoRV FbaI HindIII
HpaI KasI KpnI MamI MluI
NarI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SfoI SfuI SnaBI SpeI
SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 95 TCCGGA T^CCGGA
AflII 4291 CTTAAG C^TTAAG
BfrI 4291 CTTAAG C^TTAAG
BseAI 95 TCCGGA T^CCGGA
Bsp1407I 4183 TGTACA T^GTACA
BspEI 95 TCCGGA T^CCGGA
BsrGI 4183 TGTACA T^GTACA
BssSI 277 CTCGTG CACGAG(-5/-1)
BstEII 2289 GGTCACC G^GT[ACGT]ACC
BstPI 2289 GGTCACC G^GT[ACGT]ACC
DrdI 3451 GACTTCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2289 GGTCACC G^GT[ACGT]ACC
EcoRI 2587 GAATTC G^AATTC
EcoT22I 3645 ATGCAT ATGCA^T
FseI 87 GGCCGGCC GGCCGG^CC
MfeI 3758 CAATTG C^AATTG
MroI 95 TCCGGA T^CCGGA
MunI 3758 CAATTG C^AATTG
NsiI 3645 ATGCAT ATGCA^T
PaeR7I 2839 CTCGAG C^TCGAG
PpuMI 4304 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 1231 GAGCTC GAGCT^C
SalI 2323 GTCGAC G^TCGAC
SapI 574 GAAGAGC GCTCTTC(1/4)
SbfI 1573 CCTGCAGG CCTGCA^GG
ScaI 3891 AGTACT AGT^ACT
SexAI 2216 ACCTGGT A^CC[AT]GGT
Sse8387I 1573 CCTGCAGG CCTGCA^GG
SspBI 4183 TGTACA T^GTACA
XbaI 1311 TCTAGA T^CTAGA
XhoI 2839 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2155 GTCTAC GT^[AC][GT]AC
2323 GTCGAC
AcsI 2587 GAATTC [GA]^AATT[CT]
3520 AAATTT
AflIII 2465 ACATGT A^C[GA][CT]GT
4103 ACATGT
Alw44I 366 GTGCAC G^TGCAC
2772 GTGCAC
ApaLI 366 GTGCAC G^TGCAC
2772 GTGCAC
ApoI 2587 GAATTC [GA]^AATT[CT]
3520 AAATTT
AseI 3931 ATTAAT AT^TAAT
4138 ATTAAT
AsnI 3931 ATTAAT AT^TAAT
4138 ATTAAT
Asp700I 834 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4857 GAAGTCTTTC
AspI 760 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2677 GACTAGGTC
BbsI 766 GTCTTC GAAGAC(2/6)
1402 GTCTTC
BbvCI 1502 CCTCAGC CCTCAGC(-5/-2)
2905 GCTGAGG
BciVI 3239 GGATAC GTATCC(6/5)
4072 GGATAC
BpuAI 766 GTCTTC GAAGAC(2/6)
1402 GTCTTC
BsmI 177 GAATGC GAATGC(1/-1)
3232 GCATTC
BspLU11I 2465 ACATGT A^CATGT
4103 ACATGT
BsrBI 531 CCGCTC CCGCTC(-3/-3)
1170 GAGCGG
EarI 4397 CTCTTC CTCTTC(1/4)
574 GAAGAG
EcoNI 597 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2295 CCTACCGGAGG
Ksp632I 4397 CTCTTC CTCTTC(1/4)
574 GAAGAG
KspI 687 CCGCGG CCGC^GG
1748 CCGCGG
NcoI 2495 CCATGG C^CATGG
4235 CCATGG
PflFI 760 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2677 GACTAGGTC
PflMI 483 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2980 CCAGGCTTTGG
PshBI 3931 ATTAAT AT^TAAT
4138 ATTAAT
SacII 687 CCGCGG CCGC^GG
1748 CCGCGG
SfiI 1210 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2382 GGCCTTGGCGGCC
SgrAI 1872 CGCCGGCG C[GA]^CCGG[CT]G
2502 CACCGGCG
SphI 3041 GCATGC GCATG^C
3443 GCATGC
Tth111I 760 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2677 GACTAGGTC
Van91I 483 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2980 CCAGGCTTTGG
XcmI 1938 CCAGTCCGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4743 CCATTGCAAGTATGG
XmnI 834 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4857 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII AviII BalI
BamHI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI