##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BbeI BcgI BsaBI BsiWI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1935 TCCGGA T^CCGGA
AclI 2606 AACGTT AA^CGTT
AcyI 1437 GACGCC G[GA]^CG[CT]C
AflII 231 CTTAAG C^TTAAG
BanI 3333 GGCACC G^G[CT][GA]CC
BfrI 231 CTTAAG C^TTAAG
BglII 1503 AGATCT A^GATCT
BsaHI 1437 GACGCC G[GA]^CG[CT]C
BsaI 296 GAGACC GGTCTC(1/5)
BsaWI 1935 TCCGGA [AT]^CCGG[AT]
BseAI 1935 TCCGGA T^CCGGA
BsiEI 3764 CGACCG CG[GA][CT]^CG
BspEI 1935 TCCGGA T^CCGGA
BsrBI 4590 GAGCGG CCGCTC(-3/-3)
BsrDI 4391 CATTGC GCAATG(2/0)
BssSI 3873 CTCGTG CACGAG(-5/-1)
BstEII 4142 GGTGACC G^GT[ACGT]ACC
BstPI 4142 GGTGACC G^GT[ACGT]ACC
DraIII 2613 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4376 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4142 GGTGACC G^GT[ACGT]ACC
EcoRI 2751 GAATTC G^AATTC
Hin1I 1437 GACGCC G[GA]^CG[CT]C
MroI 1935 TCCGGA T^CCGGA
NdeI 3347 CATATG CA^TATG
PaeR7I 1720 CTCGAG C^TCGAG
Psp1406I 2606 AACGTT AA^CGTT
ScaI 4154 AGTACT AGT^ACT
SexAI 1820 ACCTGGT A^CC[AT]GGT
SphI 4760 GCATGC GCATG^C
SspI 4423 AATATT AAT^ATT
StuI 2546 AGGCCT AGG^CCT
XhoI 1720 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 47 enzymes) #
###################################
AccI 75 GTCTAC GT^[AC][GT]AC
327 GTAGAC
AccII 2650 CGCG CG^CG
3587 CGCG
AcsI 756 GAATTT [GA]^AATT[CT]
2751 GAATTC
AflIII 429 ACATGT A^C[GA][CT]GT
4576 ACATGT
AhdI 2091 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2259 GACTGGAAGTC
AocI 333 CCTAAGG CC^T[ACGT]AGG
2568 CCTGAGG
ApaI 1480 GGGCCC GGGCC^C
3087 GGGCCC
ApoI 756 GAATTT [GA]^AATT[CT]
2751 GAATTC
AspEI 2091 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2259 GACTGGAAGTC
AvrII 1634 CCTAGG C^CTAGG
2172 CCTAGG
BalI 3643 TGGCCA TGG^CCA
3772 TGGCCA
BamHI 2006 GGATCC G^GATCC
3625 GGATCC
BbrPI 3615 CACGTG CAC^GTG
5286 CACGTG
BbvCI 958 CCTCAGC CCTCAGC(-5/-2)
3145 CCTCAGC
BclI 1063 TGATCA T^GATCA
2638 TGATCA
BglI 3855 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3914 GCCTAGCCGGC
BlnI 1634 CCTAGG C^CTAGG
2172 CCTAGG
BlpI 2144 GCTTAGC GC^T[ACGT]AGC
2519 GCTGAGC
Bpu1102I 2144 GCTTAGC GC^T[ACGT]AGC
2519 GCTGAGC
BsaAI 3615 CACGTG [CT]AC^GT[GA]
5286 CACGTG
BsmBI 2448 CGTCTC CGTCTC(1/5)
2735 CGTCTC
Bsp1407I 4412 TGTACA T^GTACA
5257 TGTACA
BspLU11I 429 ACATGT A^CATGT
4576 ACATGT
BsrGI 4412 TGTACA T^GTACA
5257 TGTACA
BstUI 2650 CGCG CG^CG
3587 CGCG
BstXI 3271 CCACCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3383 CCAGACCTTTGG
Bsu36I 333 CCTAAGG CC^T[ACGT]AGG
2568 CCTGAGG
CelII 2144 GCTTAGC GC^T[ACGT]AGC
2519 GCTGAGC
Eam1105I 2091 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2259 GACTGGAAGTC
Eco81I 333 CCTAAGG CC^T[ACGT]AGG
2568 CCTGAGG
EcoT22I 3691 ATGCAT ATGCA^T
4758 ATGCAT
FbaI 1063 TGATCA T^GATCA
2638 TGATCA
HincII 3113 GTCAAC GT[CT]^[GA]AC
4038 GTTGAC
HindII 3113 GTCAAC GT[CT]^[GA]AC
4038 GTTGAC
MluNI 3643 TGGCCA TGG^CCA
3772 TGGCCA
MscI 3643 TGGCCA TGG^CCA
3772 TGGCCA
MvnI 2650 CGCG CG^CG
3587 CGCG
NaeI 2814 GCCGGC GCC^GGC
3919 GCCGGC
NcoI 3364 CCATGG C^CATGG
4088 CCATGG
NgoMIV 2814 GCCGGC G^CCGGC
3919 GCCGGC
NsiI 3691 ATGCAT ATGCA^T
4758 ATGCAT
PmaCI 3615 CACGTG CAC^GTG
5286 CACGTG
PmlI 3615 CACGTG CAC^GTG
5286 CACGTG
PpuMI 2175 AGGACCC [GA]G^G[AT]CC[CT]
2859 AGGACCC
PshAI 707 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3530 GACAGGAGTC
SspBI 4412 TGTACA T^GTACA
5257 TGTACA
XcmI 3097 CCACGCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3316 CCACTCCTATGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI ApaLI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmaCI XmaI
XmnI