##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AcyI AgeI Aor51HI
AscI AseI AsnI Asp718I AvrII
BbeI BbrPI BciVI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
BstBI BstEII BstPI ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NheI
NotI NruI NsiI NspV PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SbfI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 932 CGCG CG^CG
AclI 4789 AACGTT AA^CGTT
AflII 1441 CTTAAG C^TTAAG
AhdI 3811 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3511 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3811 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 671 TGCGCA TGC^GCA
BamHI 1092 GGATCC G^GATCC
BcgI 3111 GCAGCTACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1441 CTTAAG C^TTAAG
BssSI 3527 CTCGTG CACGAG(-5/-1)
Bst1107I 4003 GTATAC GTA^TAC
BstUI 932 CGCG CG^CG
BstXI 1823 CCAGATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4003 GTATAC GTA^TAC
DraIII 2450 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3811 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 1198 GGCCGGCC GGCCGG^CC
FspI 671 TGCGCA TGC^GCA
HaeII 1593 GGCGCT [GA]GCGC^[CT]
HgaI 5157 GACGC GACGC(5/10)
MvnI 932 CGCG CG^CG
NaeI 1199 GCCGGC GCC^GGC
NgoMIV 1199 GCCGGC G^CCGGC
PacI 5397 TTAATTAA TTAAT^TAA
PshAI 3151 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4789 AACGTT AA^CGTT
SacI 3687 GAGCTC GAGCT^C
SapI 2139 GAAGAGC GCTCTTC(1/4)
SexAI 749 ACCTGGT A^CC[AT]GGT
SfiI 954 GGCCAACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 786 CCCGGG CCC^GGG
SpeI 66 ACTAGT A^CTAGT
StuI 1926 AGGCCT AGG^CCT
SwaI 5246 ATTTAAAT ATTT^AAAT
XmaCI 786 CCCGGG C^CCGGG
XmaI 786 CCCGGG C^CCGGG
XmnI 3511 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 3211 ACATGT A^C[GA][CT]GT
4769 ACATGT
Alw44I 2285 GTGCAC G^TGCAC
4706 GTGCAC
AocI 1951 CCTGAGG CC^T[ACGT]AGG
2510 CCTCAGG
ApaLI 2285 GTGCAC G^TGCAC
4706 GTGCAC
AspI 196 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1651 GACATGGTC
AvaI 786 CCCGGG C^[CT]CG[GA]G
2129 CTCGGG
BglI 955 GCCAACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1514 GCCTCTACGGC
BsmBI 4323 CGTCTC CGTCTC(1/5)
5155 GAGACG
BsmI 3587 GAATGC GAATGC(1/-1)
1477 GCATTC
BsoBI 786 CCCGGG C^[CT]CG[GA]G
2129 CTCGGG
BspLU11I 3211 ACATGT A^CATGT
4769 ACATGT
BsrBI 630 CCGCTC CCGCTC(-3/-3)
1797 CCGCTC
BsrFI 173 ACCGGC [GA]^CCGG[CT]
1199 GCCGGC
Bsu36I 1951 CCTGAGG CC^T[ACGT]AGG
2510 CCTCAGG
Cfr10I 173 ACCGGC [GA]^CCGG[CT]
1199 GCCGGC
Eco81I 1951 CCTGAGG CC^T[ACGT]AGG
2510 CCTCAGG
EcoRI 3376 GAATTC G^AATTC
4016 GAATTC
FauI 692 CCCGC CCCGC(4/6)
2826 GCGGG
HindIII 1968 AAGCTT A^AGCTT
3825 AAGCTT
PflFI 196 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1651 GACATGGTC
PflMI 2730 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3814 CCAGGGTCTGG
PpuMI 92 AGGTCCC [GA]G^G[AT]CC[CT]
3762 AGGTCCT
SalI 470 GTCGAC G^TCGAC
3119 GTCGAC
ScaI 992 AGTACT AGT^ACT
4114 AGTACT
SspI 1266 AATATT AAT^ATT
4208 AATATT
Tth111I 196 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1651 GACATGGTC
Van91I 2730 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3814 CCAGGGTCTGG
XbaI 2475 TCTAGA T^CTAGA
5068 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspEI BspHI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII