##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AvrII BbeI BcgI BfrI
BlnI BlpI Bpu1102I BsaBI BsaI
BsiEI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI KspI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 384 GGTACC G^GTACC
AccI 4343 GTCGAC GT^[AC][GT]AC
AccII 3315 CGCG CG^CG
AccIII 1483 TCCGGA T^CCGGA
AcyI 1288 GACGCC G[GA]^CG[CT]C
AflIII 4061 ACGTGT A^C[GA][CT]GT
Alw44I 2037 GTGCAC G^TGCAC
ApaLI 2037 GTGCAC G^TGCAC
Asp718I 384 GGTACC G^GTACC
AviII 2095 TGCGCA TGC^GCA
BbrPI 4060 CACGTG CAC^GTG
BbsI 1829 GAAGAC GAAGAC(2/6)
BclI 2005 TGATCA T^GATCA
BglII 1717 AGATCT A^GATCT
BpuAI 1829 GAAGAC GAAGAC(2/6)
BsaAI 4060 CACGTG [CT]AC^GT[GA]
BsaHI 1288 GACGCC G[GA]^CG[CT]C
BseAI 1483 TCCGGA T^CCGGA
BsiWI 1967 CGTACG C^GTACG
BsmBI 3303 GAGACG CGTCTC(1/5)
BspEI 1483 TCCGGA T^CCGGA
BspHI 1153 TCATGA T^CATGA
BsrFI 2848 GCCGGC [GA]^CCGG[CT]
BstBI 718 TTCGAA TT^CGAA
BstUI 3315 CGCG CG^CG
Cfr10I 2848 GCCGGC [GA]^CCGG[CT]
DraIII 1131 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 2005 TGATCA T^GATCA
FspI 2095 TGCGCA TGC^GCA
HgaI 1288 GACGC GACGC(5/10)
Hin1I 1288 GACGCC G[GA]^CG[CT]C
KpnI 384 GGTACC GGTAC^C
MaeII 4061 ACGT A^CGT
MroI 1483 TCCGGA T^CCGGA
MvnI 3315 CGCG CG^CG
NaeI 2848 GCCGGC GCC^GGC
NcoI 20 CCATGG C^CATGG
NgoMIV 2848 GCCGGC G^CCGGC
NspV 718 TTCGAA TT^CGAA
PmaCI 4060 CACGTG CAC^GTG
PmlI 4060 CACGTG CAC^GTG
PshAI 48 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1153 TCATGA T^CATGA
SacI 1239 GAGCTC GAGCT^C
SalI 4343 GTCGAC G^TCGAC
SfuI 718 TTCGAA TT^CGAA
SphI 253 GCATGC GCATG^C
StuI 1426 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AocI 347 CCTCAGG CC^T[ACGT]AGG
2688 CCTGAGG
Asp700I 63 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4151 GAATGACTTC
BalI 17 TGGCCA TGG^CCA
3964 TGGCCA
BanI 384 GGTACC G^G[CT][GA]CC
505 GGCACC
BsgI 1128 CTGCAC GTGCAG(16/14)
4057 CTGCAC
BsmI 3283 GAATGC GAATGC(1/-1)
1595 GCATTC
BstAPI 2087 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3051 GCAGGCGATGC
Bsu36I 347 CCTCAGG CC^T[ACGT]AGG
2688 CCTGAGG
Eco81I 347 CCTCAGG CC^T[ACGT]AGG
2688 CCTGAGG
HincII 4052 GTCAAC GT[CT]^[GA]AC
4343 GTCGAC
HindII 4052 GTCAAC GT[CT]^[GA]AC
4343 GTCGAC
MluNI 17 TGGCCA TGG^CCA
3964 TGGCCA
MscI 17 TGGCCA TGG^CCA
3964 TGGCCA
SapI 1185 GAAGAGC GCTCTTC(1/4)
3186 GAAGAGC
SfiI 1767 GGCCCCTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3002 GGCCCCCTGGGCC
XmnI 63 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4151 GAATGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BamHI BanII BbvCI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI