##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BlnI
BsaHI BsmBI BspDI BspHI BsrBI
BssHII BssSI BstBI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI FspI HgaI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AocI 154 CCTTAGG CC^T[ACGT]AGG
AvaI 3898 CCCGGG C^[CT]CG[GA]G
BbrPI 2072 CACGTG CAC^GTG
BclI 944 TGATCA T^GATCA
BglI 2503 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3314 AGATCT A^GATCT
BsaAI 2072 CACGTG [CT]AC^GT[GA]
BsaBI 1524 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 11 CGACCG CG[GA][CT]^CG
BsiWI 2104 CGTACG C^GTACG
BsoBI 3898 CCCGGG C^[CT]CG[GA]G
Bsp1407I 658 TGTACA T^GTACA
BspLU11I 3012 ACATGT A^CATGT
BsrGI 658 TGTACA T^GTACA
Bst1107I 3409 GTATAC GTA^TAC
BstAPI 1926 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1460 GGTGACC G^GT[ACGT]ACC
BstPI 1460 GGTGACC G^GT[ACGT]ACC
BstZ17I 3409 GTATAC GTA^TAC
Bsu36I 154 CCTTAGG CC^T[ACGT]AGG
DraI 1653 TTTAAA TTT^AAA
DraIII 948 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1994 GACCCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 154 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1460 GGTGACC G^GT[ACGT]ACC
EcoT22I 1924 ATGCAT ATGCA^T
FbaI 944 TGATCA T^GATCA
HaeII 71 AGCGCC [GA]GCGC^[CT]
MamI 1524 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2190 GCCGGC GCC^GGC
NgoMIV 2190 GCCGGC G^CCGGC
NsiI 1924 ATGCAT ATGCA^T
PmaCI 2072 CACGTG CAC^GTG
PmlI 2072 CACGTG CAC^GTG
SacI 3483 GAGCTC GAGCT^C
SalI 78 GTCGAC G^TCGAC
SexAI 1456 ACCAGGT A^CC[AT]GGT
SmaI 3898 CCCGGG CCC^GGG
SspBI 658 TGTACA T^GTACA
SspI 2651 AATATT AAT^ATT
TaqI 79 TCGA T^CGA
TthHB8I 79 TCGA T^CGA
XmaCI 3898 CCCGGG C^CCGGG
XmaI 3898 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1235 GGTACC G^GTACC
1266 GGTACC
AccIII 193 TCCGGA T^CCGGA
2764 TCCGGA
AflII 26 CTTAAG C^TTAAG
564 CTTAAG
AflIII 173 ACGTGT A^C[GA][CT]GT
3012 ACATGT
Asp718I 1235 GGTACC G^GTACC
1266 GGTACC
BalI 2272 TGGCCA TGG^CCA
3007 TGGCCA
BcgI 80 CGACGTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1829 CGAGTTTGATGC
BfrI 26 CTTAAG C^TTAAG
564 CTTAAG
BlpI 1733 GCTGAGC GC^T[ACGT]AGC
3801 GCTGAGC
Bpu1102I 1733 GCTGAGC GC^T[ACGT]AGC
3801 GCTGAGC
BsaI 1407 GAGACC GGTCTC(1/5)
2752 GAGACC
BsaWI 193 TCCGGA [AT]^CCGG[AT]
2764 TCCGGA
BseAI 193 TCCGGA T^CCGGA
2764 TCCGGA
BsmI 888 GCATTC GAATGC(1/-1)
3352 GCATTC
BspEI 193 TCCGGA T^CCGGA
2764 TCCGGA
BsrDI 673 CATTGC GCAATG(2/0)
306 GCAATG
BsrFI 13 ACCGGC [GA]^CCGG[CT]
2190 GCCGGC
CelII 1733 GCTGAGC GC^T[ACGT]AGC
3801 GCTGAGC
Cfr10I 13 ACCGGC [GA]^CCGG[CT]
2190 GCCGGC
EcoNI 208 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3303 CCTGGTCAAGG
HincII 78 GTCGAC GT[CT]^[GA]AC
1440 GTCAAC
HindII 78 GTCGAC GT[CT]^[GA]AC
1440 GTCAAC
KpnI 1235 GGTACC GGTAC^C
1266 GGTACC
MluNI 2272 TGGCCA TGG^CCA
3007 TGGCCA
MroI 193 TCCGGA T^CCGGA
2764 TCCGGA
MscI 2272 TGGCCA TGG^CCA
3007 TGGCCA
PflMI 1205 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2264 CCAGCTGGTGG
SapI 67 GAAGAGC GCTCTTC(1/4)
2737 GCTCTTC
Van91I 1205 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2264 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII