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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AcsI AgeI
Aor51HI ApoI AscI AseI AsnI
Asp718I AvrII BciVI BclI BglII
BlnI BsiWI BsmI BspDI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI Eco47III EcoRI
EcoRV EcoT22I FbaI HincII HindII
HpaI KpnI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfuI SpeI
Sse8387I SspI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AflII 4334 CTTAAG C^TTAAG
Asp700I 1836 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 3961 CGACACTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1291 CTAG C^TAG
BfrI 4334 CTTAAG C^TTAAG
BlpI 230 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 230 GCTCAGC GC^T[ACGT]AGC
BsaBI 4347 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 600 TGTACA T^GTACA
BsrDI 3088 GCAATG GCAATG(2/0)
BsrGI 600 TGTACA T^GTACA
BstAPI 1187 GCATTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 230 GCTCAGC GC^T[ACGT]AGC
FseI 1996 GGCCGGCC GGCCGG^CC
MaeI 1291 CTAG C^TAG
MamI 4347 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
ScaI 3606 AGTACT AGT^ACT
SexAI 3056 ACCTGGT A^CC[AT]GGT
SfiI 1945 GGCCAGGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 279 TACGTA TAC^GTA
SphI 397 GCATGC GCATG^C
SspBI 600 TGTACA T^GTACA
SwaI 265 ATTTAAAT ATTT^AAAT
XmnI 1836 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3384 GTAGAC GT^[AC][GT]AC
4285 GTAGAC
AflIII 597 ACGTGT A^C[GA][CT]GT
631 ACGTGT
Alw44I 1230 GTGCAC G^TGCAC
3360 GTGCAC
AocI 3158 CCTGAGG CC^T[ACGT]AGG
4078 CCTGAGG
ApaLI 1230 GTGCAC G^TGCAC
3360 GTGCAC
AviII 1179 TGCGCA TGC^GCA
1870 TGCGCA
BalI 940 TGGCCA TGG^CCA
3062 TGGCCA
BbrPI 596 CACGTG CAC^GTG
893 CACGTG
BglI 1776 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1946 GCCAGGTCGGC
BsgI 3539 CTGCAC GTGCAG(16/14)
956 GTGCAG
BsrBI 2586 CCGCTC CCGCTC(-3/-3)
1595 GAGCGG
Bsu36I 3158 CCTGAGG CC^T[ACGT]AGG
4078 CCTGAGG
DraIII 1881 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3055 CACCTGGTG
DrdI 1323 GACCAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2974 GACGCCCATGTC
EagI 391 CGGCCG C^GGCCG
1995 CGGCCG
EclXI 391 CGGCCG C^GGCCG
1995 CGGCCG
Eco52I 391 CGGCCG C^GGCCG
1995 CGGCCG
Eco81I 3158 CCTGAGG CC^T[ACGT]AGG
4078 CCTGAGG
FspI 1179 TGCGCA TGC^GCA
1870 TGCGCA
HindIII 2053 AAGCTT A^AGCTT
4060 AAGCTT
KspI 54 CCGCGG CCGC^GG
102 CCGCGG
MluNI 940 TGGCCA TGG^CCA
3062 TGGCCA
MscI 940 TGGCCA TGG^CCA
3062 TGGCCA
NaeI 393 GCCGGC GCC^GGC
1997 GCCGGC
NgoMIV 393 GCCGGC G^CCGGC
1997 GCCGGC
PmaCI 596 CACGTG CAC^GTG
893 CACGTG
PmlI 596 CACGTG CAC^GTG
893 CACGTG
SacII 54 CCGCGG CCGC^GG
102 CCGCGG
StuI 2996 AGGCCT AGG^CCT
3535 AGGCCT
Tsp509I 1696 AATT ^AATT
3622 AATT
XcmI 1064 CCAGGGGAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2506 CCAGACCCACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AhdI AluI AlwI AlwNI ApaI
AspEI AspHI AspI AvaI AvaII
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SgrAI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI