##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AvrII BbeI BbrPI
BcgI BciVI BfrI BglII BlnI
BsaAI BsiWI BspDI BspHI BspLU11I
BspMI BsrDI Bst1107I BstBI BstZ17I
ClaI DraI DrdI EcoRV EcoT22I
HincII HindII HpaI KasI KpnI
MfeI MluI MunI NarI NcoI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3349 GTCTAC GT^[AC][GT]AC
AccIII 3898 TCCGGA T^CCGGA
AflIII 3088 ACGTGT A^C[GA][CT]GT
AhdI 4653 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4653 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 724 TGCGCA TGC^GCA
BamHI 1922 GGATCC G^GATCC
BbsI 4321 GTCTTC GAAGAC(2/6)
BbvCI 3052 CCTCAGC CCTCAGC(-5/-2)
BpuAI 4321 GTCTTC GAAGAC(2/6)
BseAI 3898 TCCGGA T^CCGGA
BsmI 3608 GAATGC GAATGC(1/-1)
Bsp1407I 4047 TGTACA T^GTACA
BspEI 3898 TCCGGA T^CCGGA
BsrGI 4047 TGTACA T^GTACA
BstEII 3930 GGTGACC G^GT[ACGT]ACC
BstPI 3930 GGTGACC G^GT[ACGT]ACC
CpoI 3895 CGGTCCG CG^G[AT]CCG
EagI 225 CGGCCG C^GGCCG
Eam1105I 4653 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 225 CGGCCG C^GGCCG
Eco52I 225 CGGCCG C^GGCCG
EcoO65I 3930 GGTGACC G^GT[ACGT]ACC
EcoRI 3639 GAATTC G^AATTC
FseI 2452 GGCCGGCC GGCCGG^CC
FspI 724 TGCGCA TGC^GCA
HindIII 1743 AAGCTT A^AGCTT
MroI 3898 TCCGGA T^CCGGA
MseI 3292 TTAA T^TAA
NdeI 3175 CATATG CA^TATG
PaeR7I 882 CTCGAG C^TCGAG
PshAI 4216 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3895 CGGTCCG CG^G[AT]CCG
SspBI 4047 TGTACA T^GTACA
Tru9I 3292 TTAA T^TAA
XhoI 882 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 3531 GTAC GT^AC
4048 GTAC
AocI 2418 CCTAAGG CC^T[ACGT]AGG
3658 CCTTAGG
Aor51HI 1261 AGCGCT AGC^GCT
1660 AGCGCT
BclI 4197 TGATCA T^GATCA
4691 TGATCA
BlpI 551 GCTCAGC GC^T[ACGT]AGC
1643 GCTGAGC
Bpu1102I 551 GCTCAGC GC^T[ACGT]AGC
1643 GCTGAGC
BsaBI 1805 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2978 GATCGGCATC
BstXI 997 CCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3860 CCAGACTTCTGG
Bsu36I 2418 CCTAAGG CC^T[ACGT]AGG
3658 CCTTAGG
CelII 551 GCTCAGC GC^T[ACGT]AGC
1643 GCTGAGC
DraIII 3339 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4684 CACTCAGTG
Eco47III 1261 AGCGCT AGC^GCT
1660 AGCGCT
Eco81I 2418 CCTAAGG CC^T[ACGT]AGG
3658 CCTTAGG
FbaI 4197 TGATCA T^GATCA
4691 TGATCA
KspI 303 CCGCGG CCGC^GG
4232 CCGCGG
MamI 1805 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2978 GATCGGCATC
MslI 728 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2855 CACCGTGGTG
NaeI 370 GCCGGC GCC^GGC
2453 GCCGGC
NgoMIV 370 GCCGGC G^CCGGC
2453 GCCGGC
NheI 1332 GCTAGC G^CTAGC
2744 GCTAGC
NspI 2887 GCATGC [GA]CATG^[CT]
4266 GCATGC
RsaI 3531 GTAC GT^AC
4048 GTAC
SacII 303 CCGCGG CCGC^GG
4232 CCGCGG
SphI 2887 GCATGC GCATG^C
4266 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI