##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AclI AcsI AflII Aor51HI
ApoI AscI AseI AsnI Asp700I
AvrII BamHI BclI BfrI BglII
BlnI BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrGI
BstBI ClaI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
FbaI HindIII HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NspV PacI PmeI PshBI
Psp1406I RcaI SalI SfiI SfuI
SpeI SspBI SspI StuI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
AatII 824 GACGTC GACGT^C
BbvCI 2998 CCTCAGC CCTCAGC(-5/-2)
BspLU11I 1948 ACATGT A^CATGT
Bst1107I 1388 GTATAC GTA^TAC
BstYI 1915 AGATCC [GA]^GATC[CT]
BstZ17I 1388 GTATAC GTA^TAC
EcoT22I 944 ATGCAT ATGCA^T
FseI 4542 GGCCGGCC GGCCGG^CC
MflI 1915 AGATCC [GA]^GATC[CT]
NsiI 944 ATGCAT ATGCA^T
PaeR7I 2393 CTCGAG C^TCGAG
PshAI 3502 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3687 CGATCG CGAT^CG
SbfI 547 CCTGCAGG CCTGCA^GG
ScaI 2409 AGTACT AGT^ACT
SnaBI 4696 TACGTA TAC^GTA
Sse8387I 547 CCTGCAGG CCTGCA^GG
Tsp509I 2422 AATT ^AATT
XhoI 2393 CTCGAG C^TCGAG
XhoII 1915 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 125 GTCTAC GT^[AC][GT]AC
1388 GTATAC
AgeI 3853 ACCGGT A^CCGGT
3994 ACCGGT
AhdI 1673 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2426 GACGATGAGTC
AocI 1835 CCTGAGG CC^T[ACGT]AGG
4513 CCTGAGG
AspEI 1673 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2426 GACGATGAGTC
AspI 2357 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4389 GACTGTGTC
BalI 771 TGGCCA TGG^CCA
3698 TGGCCA
BcgI 2915 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
30 GCAGCCACTTCG
BciVI 4693 GGATAC GTATCC(6/5)
2209 GTATCC
BglI 4538 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4592 GCCAACTGGGC
BsmI 841 GAATGC GAATGC(1/-1)
5063 GCATTC
BsrDI 4313 CATTGC GCAATG(2/0)
3414 GCAATG
BssSI 3347 CACGAG CACGAG(-5/-1)
2197 CTCGTG
BstXI 1849 CCAGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4645 CCACACCTATGG
Bsu36I 1835 CCTGAGG CC^T[ACGT]AGG
4513 CCTGAGG
Eam1105I 1673 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2426 GACGATGAGTC
Eco81I 1835 CCTGAGG CC^T[ACGT]AGG
4513 CCTGAGG
HincII 1114 GTTGAC GT[CT]^[GA]AC
1684 GTTGAC
HindII 1114 GTTGAC GT[CT]^[GA]AC
1684 GTTGAC
KspI 210 CCGCGG CCGC^GG
3743 CCGCGG
MluNI 771 TGGCCA TGG^CCA
3698 TGGCCA
MscI 771 TGGCCA TGG^CCA
3698 TGGCCA
MseI 1455 TTAA T^TAA
4640 TTAA
NaeI 2277 GCCGGC GCC^GGC
4543 GCCGGC
NcoI 3599 CCATGG C^CATGG
3636 CCATGG
NgoMIV 2277 GCCGGC G^CCGGC
4543 GCCGGC
NruI 2488 TCGCGA TCG^CGA
4344 TCGCGA
PflFI 2357 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4389 GACTGTGTC
PinAI 3853 ACCGGT A^CCGGT
3994 ACCGGT
SacII 210 CCGCGG CCGC^GG
3743 CCGCGG
SapI 1463 GAAGAGC GCTCTTC(1/4)
3296 GAAGAGC
SexAI 1445 ACCTGGT A^CC[AT]GGT
1751 ACCTGGT
SgrAI 234 CGCCGGTG C[GA]^CCGG[CT]G
4251 CGCCGGTG
TfiI 116 GATTC G^A[AT]TC
1198 GATTC
Tru9I 1455 TTAA T^TAA
4640 TTAA
Tth111I 2357 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4389 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI Asp718I AspHI AvaI
AvaII AviII BanI BanII BbeI
BbrPI BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I CpoI DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI RsrII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XbaI XcmI XmaCI
XmaI