HUGE

Gene/Protein Characteristic Table for KIAA0659

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Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00595
Accession No. :	AB014559
Description :	Sn1-specific diacylglycerol lipase alpha.
HUGO Gene Name :	diacylglycerol lipase, alpha (DAGLA)
Clone Name :	fh22726 [Vector Info]
Flexi ORF Clone :	pF1KSDA0659
Source :	Human fetal brain
Note :	We replaced hk01892, former representative clones for KIAA0659 with fh22726. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5687 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2447 bp

Genome contig ID gi51511727f_61104486
PolyA signal sequence
(TATAAA,-7)
+----*----+----*----+----*----+----
AGGAGAGACAAATTAATATAGCTTATTCTATAAAT
Flanking genome sequence
(166502 - 166551)
----+----*----+----*----+----*----+----*----+----*
ATATCTGTATATAAAGGTTTCTGTATATTGTATAGAGCTGTGTATAAACT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 11 f 61204486 61270986 20 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 1049 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9Y4D2	100.0	Sn1-specific di...	Homo sapiens
XP_001916105	98.1	similar to Sn1-...	Equus caballus
XP_601967	97.7	similar to diac...	Bos taurus
Q5YLM1	97.5	Sn1-specific di...	Rattus norvegicus
AAI48309	97.2	Diacylglycerol ...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR002921 401 541 PF01764 Lipase

ScanRegExp IPR008262 473 482 PS00120 Lipase

IPR001844 881 892 PS00296 Chaperonin Cpn60

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002921	401	541	PF01764	Lipase
ScanRegExp	IPR008262	473	482	PS00120	Lipase
IPR001844	881	892	PS00296	Chaperonin Cpn60

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 32 LFLLHTTWFVILSVVLFGLVYNP 54 PRIMARY 23

2 64 DHGRGYLGILLSCMIAEMAIIWL 86 SECONDARY 23

3 104 VLYVRLAILVIEFIYAIVGIVWL 126 PRIMARY 23

4 142 TLGMVVCNWVVILSVCITVLCVF 164 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	32	LFLLHTTWFVILSVVLFGLVYNP	54	PRIMARY	23
2	64	DHGRGYLGILLSCMIAEMAIIWL	86	SECONDARY	23
3	104	VLYVRLAILVIEFIYAIVGIVWL	126	PRIMARY	23
4	142	TLGMVVCNWVVILSVCITVLCVF	164	PRIMARY	23

Expression profile	Description

: RT-PCR Description

Experimental conditions

Primer_f
: GCATACAGAGCTTCAGCCCAG

Primer_r
: TGCCCACATTAGAGGTCCCAG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR	Description

RH mapping information	Description

Chromosome No.

: 11

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GCATACAGAGCTTCAGCCCAG
Primer_r	: TGCCCACATTAGAGGTCCCAG
PCR product length	: 186 bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	2447 bp
Genome contig ID	gi51511727f_61104486
PolyA signal sequence (TATAAA,-7)	+--------+--------+----*----+---- AGGAGAGACAAATTAATATAGCTTATTCTATAAAT
Flanking genome sequence (166502 - 166551)	----+--------+--------+--------+--------+----* ATATCTGTATATAAAGGTTTCTGTATATTGTATAGAGCTGTGTATAAACT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	11	f	61204486	61270986	20	99.4	Perfect prediction

Gene/Protein Characteristic Table for KIAA0659

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information