##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI Asp700I AviII BfrI BsiWI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoRV FspI HincII HindII HindIII
HpaI MfeI MluI MunI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SalI
SbfI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI TfiI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 4862 GGTACC G^GTACC
AccI 2391 GTCTAC GT^[AC][GT]AC
AccIII 3167 TCCGGA T^CCGGA
Alw44I 4517 GTGCAC G^TGCAC
ApaLI 4517 GTGCAC G^TGCAC
AseI 5664 ATTAAT AT^TAAT
AsnI 5664 ATTAAT AT^TAAT
Asp718I 4862 GGTACC G^GTACC
BamHI 4016 GGATCC G^GATCC
BbrPI 1930 CACGTG CAC^GTG
BsaWI 3167 TCCGGA [AT]^CCGG[AT]
BseAI 3167 TCCGGA T^CCGGA
BsmBI 5388 CGTCTC CGTCTC(1/5)
BspEI 3167 TCCGGA T^CCGGA
BspHI 4851 TCATGA T^CATGA
KpnI 4862 GGTACC GGTAC^C
MroI 3167 TCCGGA T^CCGGA
NdeI 5530 CATATG CA^TATG
NheI 3236 GCTAGC G^CTAGC
PmaCI 1930 CACGTG CAC^GTG
PmlI 1930 CACGTG CAC^GTG
PshAI 4329 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5664 ATTAAT AT^TAAT
RcaI 4851 TCATGA T^CATGA
ScaI 473 AGTACT AGT^ACT
SfiI 3255 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 115 CCCGGG CCC^GGG
SspI 5638 AATATT AAT^ATT
XmaCI 115 CCCGGG C^CCGGG
XmaI 115 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 3646 ACATGT A^C[GA][CT]GT
3758 ACATGT
AvrII 4156 CCTAGG C^CTAGG
4820 CCTAGG
BbeI 1175 GGCGCC GGCGC^C
2810 GGCGCC
BciVI 1298 GTATCC GTATCC(6/5)
1575 GTATCC
BglII 3696 AGATCT A^GATCT
5110 AGATCT
BlnI 4156 CCTAGG C^CTAGG
4820 CCTAGG
BsaBI 330 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1293 GATCAGTATC
BsmI 3145 GAATGC GAATGC(1/-1)
3538 GCATTC
Bsp1407I 2477 TGTACA T^GTACA
3755 TGTACA
BspLU11I 3646 ACATGT A^CATGT
3758 ACATGT
BsrGI 2477 TGTACA T^GTACA
3755 TGTACA
BstEII 921 GGTGACC G^GT[ACGT]ACC
1830 GGTGACC
BstPI 921 GGTGACC G^GT[ACGT]ACC
1830 GGTGACC
DraIII 2539 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3294 CACCTGGTG
EcoO65I 921 GGTGACC G^GT[ACGT]ACC
1830 GGTGACC
EcoT22I 1794 ATGCAT ATGCA^T
4501 ATGCAT
FseI 2774 GGCCGGCC GGCCGG^CC
3712 GGCCGGCC
KasI 1175 GGCGCC G^GCGCC
2810 GGCGCC
MamI 330 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1293 GATCAGTATC
NarI 1175 GGCGCC GG^CGCC
2810 GGCGCC
NsiI 1794 ATGCAT ATGCA^T
4501 ATGCAT
SapI 3626 GAAGAGC GCTCTTC(1/4)
228 GCTCTTC
SfoI 1175 GGCGCC GGC^GCC
2810 GGCGCC
SphI 350 GCATGC GCATG^C
4481 GCATGC
SspBI 2477 TGTACA T^GTACA
3755 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII