##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI BamHI BbeI BbvCI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaBI BsiEI BsiWI BspDI
BspHI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI HpaI KasI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 3659 GGTACC G^GTACC
AccI 2871 GTCTAC GT^[AC][GT]AC
AccIII 3192 TCCGGA T^CCGGA
AclI 4152 AACGTT AA^CGTT
AcsI 3438 AAATTC [GA]^AATT[CT]
AflIII 486 ACATGT A^C[GA][CT]GT
ApoI 3438 AAATTC [GA]^AATT[CT]
Asp718I 3659 GGTACC G^GTACC
BbrPI 2727 CACGTG CAC^GTG
BcgI 2245 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2727 CACGTG [CT]AC^GT[GA]
BsaWI 3192 TCCGGA [AT]^CCGG[AT]
BseAI 3192 TCCGGA T^CCGGA
BsmBI 1242 GAGACG CGTCTC(1/5)
BspEI 3192 TCCGGA T^CCGGA
BspLU11I 486 ACATGT A^CATGT
BstYI 2749 AGATCC [GA]^GATC[CT]
DrdI 3621 GACACCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 2927 GTTGAC GT[CT]^[GA]AC
HindII 2927 GTTGAC GT[CT]^[GA]AC
KpnI 3659 GGTACC GGTAC^C
KspI 85 CCGCGG CCGC^GG
MflI 2749 AGATCC [GA]^GATC[CT]
MroI 3192 TCCGGA T^CCGGA
PmaCI 2727 CACGTG CAC^GTG
PmlI 2727 CACGTG CAC^GTG
Psp1406I 4152 AACGTT AA^CGTT
SacII 85 CCGCGG CCGC^GG
SapI 3972 GAAGAGC GCTCTTC(1/4)
ScaI 862 AGTACT AGT^ACT
SgrAI 119 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1894 CCCGGG CCC^GGG
XbaI 4113 TCTAGA T^CTAGA
XhoII 2749 AGATCC [GA]^GATC[CT]
XmaCI 1894 CCCGGG C^CCGGG
XmaI 1894 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AviII 1477 TGCGCA TGC^GCA
2644 TGCGCA
AvrII 1955 CCTAGG C^CTAGG
3311 CCTAGG
BlnI 1955 CCTAGG C^CTAGG
3311 CCTAGG
BsmI 1097 GAATGC GAATGC(1/-1)
2278 GCATTC
Bsp1407I 730 TGTACA T^GTACA
2116 TGTACA
BsrGI 730 TGTACA T^GTACA
2116 TGTACA
BssSI 670 CTCGTG CACGAG(-5/-1)
2782 CTCGTG
BstAPI 594 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3681 GCATCACTTGC
DraIII 595 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3989 CACCCTGTG
EcoT22I 3418 ATGCAT ATGCA^T
3828 ATGCAT
FspI 1477 TGCGCA TGC^GCA
2644 TGCGCA
HindIII 1611 AAGCTT A^AGCTT
3272 AAGCTT
NaeI 120 GCCGGC GCC^GGC
572 GCCGGC
NcoI 3126 CCATGG C^CATGG
3200 CCATGG
NgoMIV 120 GCCGGC G^CCGGC
572 GCCGGC
NsiI 3418 ATGCAT ATGCA^T
3828 ATGCAT
SspBI 730 TGTACA T^GTACA
2116 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmnI