##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
ApaLI AscI AviII BbvCI BclI
BfrI BsaBI BseAI BspDI BspEI
BspHI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI DrdI
EcoO65I EcoRI EcoRV FbaI FspI
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NheI NotI
NspV PacI PinAI PmeI Psp1406I
RcaI SacII SfiI SfuI SgrAI
SnaBI SphI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 813 GGTACC G^GTACC
AhdI 4210 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3135 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 987 CCTCAGG CC^T[ACGT]AGG
Aor51HI 885 AGCGCT AGC^GCT
AseI 4789 ATTAAT AT^TAAT
AsnI 4789 ATTAAT AT^TAAT
Asp718I 813 GGTACC G^GTACC
AspEI 4210 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4934 CCTAGG C^CTAGG
BamHI 3361 GGATCC G^GATCC
BcgI 4476 CGAGTTTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4728 GGATAC GTATCC(6/5)
BglII 4644 AGATCT A^GATCT
BlnI 4934 CCTAGG C^CTAGG
BsaI 2831 GAGACC GGTCTC(1/5)
BsaWI 1390 ACCGGA [AT]^CCGG[AT]
BsiWI 3263 CGTACG C^GTACG
BspLU11I 5112 ACATGT A^CATGT
BssHII 2364 GCGCGC G^CGCGC
BstXI 648 CCAGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 987 CCTCAGG CC^T[ACGT]AGG
CpoI 124 CGGACCG CG^G[AT]CCG
DraIII 4748 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2281 CGGCCG C^GGCCG
Eam1105I 4210 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2281 CGGCCG C^GGCCG
Eco47III 885 AGCGCT AGC^GCT
Eco52I 2281 CGGCCG C^GGCCG
Eco81I 987 CCTCAGG CC^T[ACGT]AGG
EcoT22I 714 ATGCAT ATGCA^T
FseI 855 GGCCGGCC GGCCGG^CC
KpnI 813 GGTACC GGTAC^C
NdeI 1083 CATATG CA^TATG
NruI 14 TCGCGA TCG^CGA
NsiI 714 ATGCAT ATGCA^T
PaeR7I 623 CTCGAG C^TCGAG
PshAI 523 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4789 ATTAAT AT^TAAT
PvuI 1259 CGATCG CGAT^CG
RsrII 124 CGGACCG CG^G[AT]CCG
SbfI 2449 CCTGCAGG CCTGCA^GG
ScaI 744 AGTACT AGT^ACT
SexAI 930 ACCAGGT A^CC[AT]GGT
SpeI 2 ACTAGT A^CTAGT
Sse8387I 2449 CCTGCAGG CCTGCA^GG
SspI 5235 AATATT AAT^ATT
XbaI 8 TCTAGA T^CTAGA
XhoI 623 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 935 GTCGAC GT^[AC][GT]AC
2600 GTCGAC
AflIII 2076 ACGTGT A^C[GA][CT]GT
5112 ACATGT
AspI 929 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4925 GACGCAGTC
BbeI 163 GGCGCC GGCGC^C
800 GGCGCC
BbrPI 818 CACGTG CAC^GTG
2479 CACGTG
BglI 1579 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4105 GCCCTGCTGGC
BlpI 4045 GCTCAGC GC^T[ACGT]AGC
4078 GCTAAGC
Bpu1102I 4045 GCTCAGC GC^T[ACGT]AGC
4078 GCTAAGC
BsmBI 1458 CGTCTC CGTCTC(1/5)
3228 CGTCTC
Bsp1407I 4814 TGTACA T^GTACA
5115 TGTACA
BsrBI 3374 CCGCTC CCGCTC(-3/-3)
18 GAGCGG
BsrDI 717 CATTGC GCAATG(2/0)
2286 GCAATG
BsrGI 4814 TGTACA T^GTACA
5115 TGTACA
CelII 4045 GCTCAGC GC^T[ACGT]AGC
4078 GCTAAGC
HincII 935 GTCGAC GT[CT]^[GA]AC
2600 GTCGAC
HindII 935 GTCGAC GT[CT]^[GA]AC
2600 GTCGAC
KasI 163 GGCGCC G^GCGCC
800 GGCGCC
NarI 163 GGCGCC GG^CGCC
800 GGCGCC
NspI 1782 ACATGC [GA]CATG^[CT]
5112 ACATGT
PflFI 929 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4925 GACGCAGTC
PmaCI 818 CACGTG CAC^GTG
2479 CACGTG
PmlI 818 CACGTG CAC^GTG
2479 CACGTG
SalI 935 GTCGAC G^TCGAC
2600 GTCGAC
SapI 2621 GAAGAGC GCTCTTC(1/4)
301 GCTCTTC
SfoI 163 GGCGCC GGC^GCC
800 GGCGCC
SspBI 4814 TGTACA T^GTACA
5115 TGTACA
TfiI 3845 GATTC G^A[AT]TC
4672 GAATC
Tth111I 929 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4925 GACGCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI