##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BglII BlnI BsaHI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstPI BstUI Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccIII 803 TCCGGA T^CCGGA
AflII 1204 CTTAAG C^TTAAG
AflIII 256 ACATGT A^C[GA][CT]GT
Alw44I 1618 GTGCAC G^TGCAC
ApaLI 1618 GTGCAC G^TGCAC
AseI 682 ATTAAT AT^TAAT
AsnI 682 ATTAAT AT^TAAT
AvaI 16 CCCGGG C^[CT]CG[GA]G
BalI 3856 TGGCCA TGG^CCA
BamHI 2586 GGATCC G^GATCC
BanI 2030 GGCACC G^G[CT][GA]CC
BbvCI 610 CCTCAGC CCTCAGC(-5/-2)
BcgI 352 CGACAGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1494 GGATAC GTATCC(6/5)
BfrI 1204 CTTAAG C^TTAAG
BglI 2302 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3181 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3181 GCTGAGC GC^T[ACGT]AGC
BsaAI 3951 CACGTA [CT]AC^GT[GA]
BsaBI 709 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 803 TCCGGA [AT]^CCGG[AT]
BseAI 803 TCCGGA T^CCGGA
BsmBI 1700 CGTCTC CGTCTC(1/5)
BsoBI 16 CCCGGG C^[CT]CG[GA]G
BspEI 803 TCCGGA T^CCGGA
BspLU11I 256 ACATGT A^CATGT
BsrDI 2250 CATTGC GCAATG(2/0)
BstAPI 1611 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2681 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3181 GCTGAGC GC^T[ACGT]AGC
DsaI 4150 CCATGG C^C[GA][CT]GG
EaeI 3856 TGGCCA [CT]^GGCC[GA]
EcoRI 3395 GAATTC G^AATTC
FauI 2328 CCCGC CCCGC(4/6)
HaeII 341 GGCGCT [GA]GCGC^[CT]
HgaI 1629 GACGC GACGC(5/10)
HincII 1858 GTTGAC GT[CT]^[GA]AC
HindII 1858 GTTGAC GT[CT]^[GA]AC
HindIII 3167 AAGCTT A^AGCTT
MamI 709 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3856 TGGCCA TGG^CCA
MroI 803 TCCGGA T^CCGGA
MscI 3856 TGGCCA TGG^CCA
MslI 2007 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 4150 CCATGG C^CATGG
NheI 556 GCTAGC G^CTAGC
PflMI 2064 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 795 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 682 ATTAAT AT^TAAT
SacI 1674 GAGCTC GAGCT^C
SapI 3262 GAAGAGC GCTCTTC(1/4)
SbfI 1588 CCTGCAGG CCTGCA^GG
ScaI 3435 AGTACT AGT^ACT
SmaI 16 CCCGGG CCC^GGG
SphI 212 GCATGC GCATG^C
Sse8387I 1588 CCTGCAGG CCTGCA^GG
TaqI 351 TCGA T^CGA
TthHB8I 351 TCGA T^CGA
Van91I 2064 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2742 TCTAGA T^CTAGA
XcmI 3525 CCAGTATTTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 16 CCCGGG C^CCGGG
XmaI 16 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 2766 GTATAC GT^[AC][GT]AC
3662 GTATAC
AlwNI 962 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2351 CAGTCACTG
BanII 952 GAGCCC G[GA]GC[CT]^C
1674 GAGCTC
BsaI 1846 GAGACC GGTCTC(1/5)
1891 GAGACC
BseRI 1404 CTCCTC GAGGAG(10/8)
1582 GAGGAG
BssSI 129 CACGAG CACGAG(-5/-1)
1708 CACGAG
Bst1107I 2766 GTATAC GTA^TAC
3662 GTATAC
BstYI 1277 AGATCC [GA]^GATC[CT]
2586 GGATCC
BstZ17I 2766 GTATAC GTA^TAC
3662 GTATAC
CfoI 342 GCGC GCG^C
452 GCGC
DrdI 2344 GACAAATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3886 GACCCACCTGTC
EcoNI 35 CCTACCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
217 CCTAATCAAGG
HhaI 342 GCGC GCG^C
452 GCGC
HinP1I 342 GCGC G^CGC
452 GCGC
MflI 1277 AGATCC [GA]^GATC[CT]
2586 GGATCC
SspI 3031 AATATT AAT^ATT
3586 AATATT
XhoII 1277 AGATCC [GA]^GATC[CT]
2586 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI AvaII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XmnI