##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AclI AcsI AflII ApoI
AscI AseI AsnI Asp700I AviII
BciVI BfrI BglII BsaBI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BstBI ClaI CpoI DraI DraIII
EcoRI EcoRV EcoT22I FspI HindIII
HpaI MamI MfeI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SexAI SfuI SnaBI SpeI
SspI SwaI Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 862 GACGTC GACGT^C
AgeI 1834 ACCGGT A^CCGGT
Aor51HI 2918 AGCGCT AGC^GCT
AspI 1273 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 154 CCTAGG C^CTAGG
BbrPI 3073 CACGTG CAC^GTG
BlnI 154 CCTAGG C^CTAGG
BlpI 2791 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2791 GCTGAGC GC^T[ACGT]AGC
BsiEI 1430 CGGCCG CG[GA][CT]^CG
BsmBI 2529 CGTCTC CGTCTC(1/5)
BsmI 3794 GCATTC GAATGC(1/-1)
Bsp1407I 3921 TGTACA T^GTACA
BsrBI 3179 CCGCTC CCGCTC(-3/-3)
BsrGI 3921 TGTACA T^GTACA
Bst1107I 4052 GTATAC GTA^TAC
BstEII 3308 GGTCACC G^GT[ACGT]ACC
BstPI 3308 GGTCACC G^GT[ACGT]ACC
BstZ17I 4052 GTATAC GTA^TAC
CelII 2791 GCTGAGC GC^T[ACGT]AGC
EagI 1430 CGGCCG C^GGCCG
EclXI 1430 CGGCCG C^GGCCG
Eco47III 2918 AGCGCT AGC^GCT
Eco52I 1430 CGGCCG C^GGCCG
EcoO65I 3308 GGTCACC G^GT[ACGT]ACC
FseI 605 GGCCGGCC GGCCGG^CC
HincII 859 GTCGAC GT[CT]^[GA]AC
HindII 859 GTCGAC GT[CT]^[GA]AC
MluI 2974 ACGCGT A^CGCGT
PflFI 1273 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1834 ACCGGT A^CCGGT
PmaCI 3073 CACGTG CAC^GTG
PmlI 3073 CACGTG CAC^GTG
SalI 859 GTCGAC G^TCGAC
ScaI 1118 AGTACT AGT^ACT
SgrAI 1833 CACCGGTG C[GA]^CCGG[CT]G
SmlI 2290 CTCAAG C^T[CT][GA]AG
SphI 2769 GCATGC GCATG^C
SspBI 3921 TGTACA T^GTACA
StuI 1501 AGGCCT AGG^CCT
Tsp509I 4022 AATT ^AATT
Tth111I 1273 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 14 GGTACC G^GTACC
1688 GGTACC
AflIII 2974 ACGCGT A^C[GA][CT]GT
3864 ACGTGT
AhdI 532 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1953 GACCCGGCGTC
Alw44I 1381 GTGCAC G^TGCAC
1879 GTGCAC
AocI 18 CCTCAGG CC^T[ACGT]AGG
1854 CCTGAGG
ApaLI 1381 GTGCAC G^TGCAC
1879 GTGCAC
Asp718I 14 GGTACC G^GTACC
1688 GGTACC
AspEI 532 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1953 GACCCGGCGTC
BamHI 137 GGATCC G^GATCC
1125 GGATCC
BcgI 1470 CGACCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2000 GCATTGAGATCG
BclI 1145 TGATCA T^GATCA
4017 TGATCA
BsaAI 3073 CACGTG [CT]AC^GT[GA]
3863 TACGTG
BsaWI 1834 ACCGGT [AT]^CCGG[AT]
2060 TCCGGT
BsrDI 1770 CATTGC GCAATG(2/0)
3290 CATTGC
BssSI 2203 CACGAG CACGAG(-5/-1)
2783 CTCGTG
BstAPI 1411 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1872 GCAGCTGGTGC
BstXI 1370 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2695 CCAACACCCTGG
Bsu36I 18 CCTCAGG CC^T[ACGT]AGG
1854 CCTGAGG
Eam1105I 532 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1953 GACCCGGCGTC
Eco81I 18 CCTCAGG CC^T[ACGT]AGG
1854 CCTGAGG
EcoNI 1854 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3552 CCTCCTAAAGG
FbaI 1145 TGATCA T^GATCA
4017 TGATCA
KpnI 14 GGTACC GGTAC^C
1688 GGTACC
MseI 1111 TTAA T^TAA
4028 TTAA
NspI 2769 GCATGC [GA]CATG^[CT]
3666 GCATGT
SapI 1995 GAAGAGC GCTCTTC(1/4)
2161 GAAGAGC
SbfI 2337 CCTGCAGG CCTGCA^GG
2586 CCTGCAGG
Sse8387I 2337 CCTGCAGG CCTGCA^GG
2586 CCTGCAGG
Tru9I 1111 TTAA T^TAA
4028 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI