##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BlnI BpuAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KspI
MfeI MluI MunI MvnI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Van91I XcmI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 3886 GGTACC G^GTACC
AccI 1711 GTAGAC GT^[AC][GT]AC
AccIII 2796 TCCGGA T^CCGGA
Aor51HI 1043 AGCGCT AGC^GCT
Asp718I 3886 GGTACC G^GTACC
BbeI 3853 GGCGCC GGCGC^C
BbvCI 573 GCTGAGG CCTCAGC(-5/-2)
BsaAI 1687 CACGTA [CT]AC^GT[GA]
BsaWI 2796 TCCGGA [AT]^CCGG[AT]
BseAI 2796 TCCGGA T^CCGGA
BsmI 195 GCATTC GAATGC(1/-1)
BspEI 2796 TCCGGA T^CCGGA
BsrFI 1573 GCCGGT [GA]^CCGG[CT]
BssSI 1705 CTCGTG CACGAG(-5/-1)
BstAPI 4313 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1573 GCCGGT [GA]^CCGG[CT]
Eco47III 1043 AGCGCT AGC^GCT
EcoNI 935 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2119 GAATTC G^AATTC
HindIII 328 AAGCTT A^AGCTT
KasI 3853 GGCGCC G^GCGCC
KpnI 3886 GGTACC GGTAC^C
MroI 2796 TCCGGA T^CCGGA
NarI 3853 GGCGCC GG^CGCC
NheI 3859 GCTAGC G^CTAGC
NspI 1988 GCATGT [GA]CATG^[CT]
PaeR7I 304 CTCGAG C^TCGAG
SfiI 3560 GGCCGAACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3853 GGCGCC GGC^GCC
XbaI 1602 TCTAGA T^CTAGA
XhoI 304 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1957 GGCGTC G[GA]^CG[CT]C
3853 GGCGCC
AocI 363 CCTGAGG CC^T[ACGT]AGG
611 CCTAAGG
ApaI 3354 GGGCCC GGGCC^C
4281 GGGCCC
BglI 2239 GCCCGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3561 GCCGAACGGGC
BlpI 428 GCTCAGC GC^T[ACGT]AGC
1413 GCTCAGC
Bpu1102I 428 GCTCAGC GC^T[ACGT]AGC
1413 GCTCAGC
BsaBI 1606 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1732 GATCAACATC
BsaHI 1957 GGCGTC G[GA]^CG[CT]C
3853 GGCGCC
BsgI 3042 CTGCAC GTGCAG(16/14)
3186 CTGCAC
BsrDI 2988 CATTGC GCAATG(2/0)
2227 GCAATG
BstEII 65 GGTGACC G^GT[ACGT]ACC
4240 GGTCACC
BstPI 65 GGTGACC G^GT[ACGT]ACC
4240 GGTCACC
Bsu36I 363 CCTGAGG CC^T[ACGT]AGG
611 CCTAAGG
CelII 428 GCTCAGC GC^T[ACGT]AGC
1413 GCTCAGC
Eco81I 363 CCTGAGG CC^T[ACGT]AGG
611 CCTAAGG
EcoO65I 65 GGTGACC G^GT[ACGT]ACC
4240 GGTCACC
Hin1I 1957 GGCGTC G[GA]^CG[CT]C
3853 GGCGCC
MamI 1606 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1732 GATCAACATC
MslI 2508 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3830 CACACCTGTG
NcoI 1156 CCATGG C^CATGG
2155 CCATGG
PpuMI 2562 GGGACCC [GA]G^G[AT]CC[CT]
3372 AGGTCCC
SacI 426 GAGCTC GAGCT^C
2276 GAGCTC
SapI 1510 GAAGAGC GCTCTTC(1/4)
3609 GAAGAGC
SmaI 2854 CCCGGG CCC^GGG
3229 CCCGGG
SmlI 304 CTCGAG C^T[CT][GA]AG
936 CTTGAG
XmaCI 2854 CCCGGG C^CCGGG
3229 CCCGGG
XmaI 2854 CCCGGG C^CCGGG
3229 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmnI