##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
BamHI BbeI BbrPI BclI BfrI
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspEI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
HaeII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI PacI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Van91I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 958 CGCG CG^CG
AcyI 3638 GACGCC G[GA]^CG[CT]C
AflIII 4175 ACATGT A^C[GA][CT]GT
AgeI 3439 ACCGGT A^CCGGT
ApaI 3160 GGGCCC GGGCC^C
Asp700I 2903 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1257 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3189 CCTAGG C^CTAGG
BciVI 3199 GTATCC GTATCC(6/5)
BglII 2085 AGATCT A^GATCT
BlnI 3189 CCTAGG C^CTAGG
BsaHI 3638 GACGCC G[GA]^CG[CT]C
BsmBI 974 CGTCTC CGTCTC(1/5)
BspDI 293 ATCGAT AT^CGAT
BspLU11I 4175 ACATGT A^CATGT
BstBI 1601 TTCGAA TT^CGAA
BstUI 958 CGCG CG^CG
ClaI 293 ATCGAT AT^CGAT
DraI 3559 TTTAAA TTT^AAA
DraIII 3790 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2388 GACTCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 287 ATGCAT ATGCA^T
HgaI 3638 GACGC GACGC(5/10)
Hin1I 3638 GACGCC G[GA]^CG[CT]C
MvnI 958 CGCG CG^CG
NaeI 1284 GCCGGC GCC^GGC
NgoMIV 1284 GCCGGC G^CCGGC
NsiI 287 ATGCAT ATGCA^T
NspV 1601 TTCGAA TT^CGAA
PaeR7I 2390 CTCGAG C^TCGAG
PflFI 1257 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3439 ACCGGT A^CCGGT
SacI 2131 GAGCTC GAGCT^C
SfuI 1601 TTCGAA TT^CGAA
SphI 1941 GCATGC GCATG^C
SspI 3824 AATATT AAT^ATT
Tth111I 1257 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2390 CTCGAG C^TCGAG
XmnI 2903 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 2390 CTCGAG C^[CT]CG[GA]G
3349 CCCGAG
AviII 1992 TGCGCA TGC^GCA
2527 TGCGCA
BalI 141 TGGCCA TGG^CCA
3848 TGGCCA
BlpI 2440 GCTGAGC GC^T[ACGT]AGC
3250 GCTAAGC
Bpu1102I 2440 GCTGAGC GC^T[ACGT]AGC
3250 GCTAAGC
BsaI 1998 GGTCTC GGTCTC(1/5)
4098 GGTCTC
BsaWI 2186 ACCGGA [AT]^CCGG[AT]
3439 ACCGGT
BsgI 2113 GTGCAG GTGCAG(16/14)
4143 GTGCAG
BsmI 776 GAATGC GAATGC(1/-1)
3811 GCATTC
BsoBI 2390 CTCGAG C^[CT]CG[GA]G
3349 CCCGAG
BsrDI 549 CATTGC GCAATG(2/0)
43 GCAATG
BstAPI 280 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1351 GCACCTTCTGC
BstYI 1784 AGATCC [GA]^GATC[CT]
2085 AGATCT
CelII 2440 GCTGAGC GC^T[ACGT]AGC
3250 GCTAAGC
CfoI 1993 GCGC GCG^C
2528 GCGC
EcoNI 184 CCTAAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2217 CCTGGAGGAGG
FspI 1992 TGCGCA TGC^GCA
2527 TGCGCA
HhaI 1993 GCGC GCG^C
2528 GCGC
HinP1I 1993 GCGC G^CGC
2528 GCGC
HincII 392 GTCAAC GT[CT]^[GA]AC
4148 GTCAAC
HindII 392 GTCAAC GT[CT]^[GA]AC
4148 GTCAAC
MflI 1784 AGATCC [GA]^GATC[CT]
2085 AGATCT
MluNI 141 TGGCCA TGG^CCA
3848 TGGCCA
MscI 141 TGGCCA TGG^CCA
3848 TGGCCA
PpuMI 596 AGGACCT [GA]G^G[AT]CC[CT]
3359 GGGACCC
SapI 2890 GAAGAGC GCTCTTC(1/4)
1060 GCTCTTC
SexAI 2720 ACCAGGT A^CC[AT]GGT
3878 ACCTGGT
XhoII 1784 AGATCC [GA]^GATC[CT]
2085 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI Bsu36I
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I