##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoT22I FauI FseI FspI
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI MvnI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I StuI
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Alw44I 4209 GTGCAC G^TGCAC
AocI 2904 CCTCAGG CC^T[ACGT]AGG
ApaLI 4209 GTGCAC G^TGCAC
AspI 2155 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3096 CCCGGG C^[CT]CG[GA]G
AvrII 1833 CCTAGG C^CTAGG
BalI 3117 TGGCCA TGG^CCA
BciVI 1026 GTATCC GTATCC(6/5)
BclI 1041 TGATCA T^GATCA
BglI 2869 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1833 CCTAGG C^CTAGG
BlpI 2330 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2330 GCTCAGC GC^T[ACGT]AGC
BsaAI 2671 TACGTA [CT]AC^GT[GA]
BsaI 1687 GGTCTC GGTCTC(1/5)
BsoBI 3096 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1081 TGTACA T^GTACA
BsrFI 3734 GCCGGC [GA]^CCGG[CT]
BsrGI 1081 TGTACA T^GTACA
BstAPI 4202 GCACATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3724 GGTCACC G^GT[ACGT]ACC
BstPI 3724 GGTCACC G^GT[ACGT]ACC
Bsu36I 2904 CCTCAGG CC^T[ACGT]AGG
CelII 2330 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3734 GCCGGC [GA]^CCGG[CT]
DraIII 4203 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3655 CCATGG C^C[GA][CT]GG
EaeI 3117 TGGCCA [CT]^GGCC[GA]
Eco81I 2904 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3724 GGTCACC G^GT[ACGT]ACC
EcoRI 3715 GAATTC G^AATTC
FbaI 1041 TGATCA T^GATCA
HaeII 3165 AGCGCC [GA]GCGC^[CT]
HpaI 271 GTTAAC GTT^AAC
MluNI 3117 TGGCCA TGG^CCA
MscI 3117 TGGCCA TGG^CCA
MspA1I 2333 CAGCTG C[AC]G^C[GT]G
NaeI 3734 GCCGGC GCC^GGC
NcoI 3655 CCATGG C^CATGG
NdeI 2165 CATATG CA^TATG
NgoMIV 3734 GCCGGC G^CCGGC
PflFI 2155 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 2333 CAGCTG CAG^CTG
SmaI 3096 CCCGGG CCC^GGG
SnaBI 2671 TACGTA TAC^GTA
SspBI 1081 TGTACA T^GTACA
SwaI 1556 ATTTAAAT ATTT^AAAT
TaqI 1353 TCGA T^CGA
Tth111I 2155 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1353 TCGA T^CGA
XbaI 2760 TCTAGA T^CTAGA
XmaCI 3096 CCCGGG C^CCGGG
XmaI 3096 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 593 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3164 CAGCGCCTG
BcnI 3096 CCCGG CC^[GC]GG
3097 CCGGG
BsaBI 624 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1810 GATTACCATC
BseRI 1382 CTCCTC GAGGAG(10/8)
2984 CTCCTC
BsmBI 3105 CGTCTC CGTCTC(1/5)
3176 GAGACG
BsmI 2865 GAATGC GAATGC(1/-1)
2899 GCATTC
BspHI 3221 TCATGA T^CATGA
3582 TCATGA
BspMI 4198 ACCTGC ACCTGC(4/8)
3196 GCAGGT
BsrDI 1107 GCAATG GCAATG(2/0)
2269 GCAATG
DraII 2038 GGGACCT [GA]G^G[ACGT]CC[CT]
3510 GGGTCCT
EcoO109I 2038 GGGACCT [GA]G^G[ACGT]CC[CT]
3510 GGGTCCT
EcoRV 1206 GATATC GAT^ATC
1657 GATATC
HaeIII 3118 GGCC GG^CC
3494 GGCC
HapII 3097 CCGG C^CGG
3735 CCGG
HgaI 3178 GACGC GACGC(5/10)
2559 GCGTC
HincII 224 GTCAAC GT[CT]^[GA]AC
271 GTTAAC
HindII 224 GTCAAC GT[CT]^[GA]AC
271 GTTAAC
HindIII 741 AAGCTT A^AGCTT
1288 AAGCTT
HpaII 3097 CCGG C^CGG
3735 CCGG
MamI 624 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1810 GATTACCATC
MspI 3097 CCGG C^CGG
3735 CCGG
NciI 3096 CCCGG CC^[GC]GG
3097 CCGGG
PpuMI 2038 GGGACCT [GA]G^G[AT]CC[CT]
3510 GGGTCCT
PstI 1625 CTGCAG CTGCA^G
3086 CTGCAG
RcaI 3221 TCATGA T^CATGA
3582 TCATGA
SmlI 2955 CTTGAG C^T[CT][GA]AG
4085 CTCAAG
SspI 517 AATATT AAT^ATT
1359 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmnI