##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I BamHI BfrI BsiWI
BsmI BspDI BspHI Bst1107I BstZ17I
ClaI CpoI Eco47III EcoRI EcoT22I
HindIII HpaI KpnI KspI MfeI
MunI NdeI NotI NruI NsiI
PacI PaeR7I PinAI PshBI Psp1406I
PvuI RcaI RsrII SacII SgrAI
SnaBI SpeI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 3022 TCCGGA T^CCGGA
AcsI 2354 AAATTC [GA]^AATT[CT]
AhdI 3485 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4001 GGGCCC GGGCC^C
ApoI 2354 AAATTC [GA]^AATT[CT]
AspEI 3485 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 830 CCTAGG C^CTAGG
BbrPI 4359 CACGTG CAC^GTG
BciVI 1560 GTATCC GTATCC(6/5)
BclI 2730 TGATCA T^GATCA
BlnI 830 CCTAGG C^CTAGG
BsaAI 4359 CACGTG [CT]AC^GT[GA]
BseAI 3022 TCCGGA T^CCGGA
BsiEI 2569 CGGCCG CG[GA][CT]^CG
Bsp1407I 4355 TGTACA T^GTACA
BspEI 3022 TCCGGA T^CCGGA
BsrGI 4355 TGTACA T^GTACA
BssHII 3593 GCGCGC G^CGCGC
BstAPI 4058 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1081 GGTGACC G^GT[ACGT]ACC
BstPI 1081 GGTGACC G^GT[ACGT]ACC
DrdI 236 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2569 CGGCCG C^GGCCG
Eam1105I 3485 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2569 CGGCCG C^GGCCG
Eco52I 2569 CGGCCG C^GGCCG
EcoO65I 1081 GGTGACC G^GT[ACGT]ACC
EcoRV 353 GATATC GAT^ATC
FbaI 2730 TGATCA T^GATCA
FseI 2566 GGCCGGCC GGCCGG^CC
MluI 644 ACGCGT A^CGCGT
MroI 3022 TCCGGA T^CCGGA
NheI 2534 GCTAGC G^CTAGC
PmaCI 4359 CACGTG CAC^GTG
PmeI 2395 GTTTAAAC GTTT^AAAC
PmlI 4359 CACGTG CAC^GTG
PshAI 1084 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2134 CCTGCAGG CCTGCA^GG
ScaI 2541 AGTACT AGT^ACT
SphI 1902 GCATGC GCATG^C
Sse8387I 2134 CCTGCAGG CCTGCA^GG
SspBI 4355 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Asp700I 1405 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3232 GAAGAAGTTC
AspI 325 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3661 GACGGAGTC
AviII 1369 TGCGCA TGC^GCA
4339 TGCGCA
BbeI 1378 GGCGCC GGCGC^C
3985 GGCGCC
BbvCI 1345 CCTCAGC CCTCAGC(-5/-2)
2062 CCTCAGC
BcgI 1723 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3034 GCACTACCTTCG
BglII 2917 AGATCT A^GATCT
4093 AGATCT
BsaBI 1003 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2918 GATCTGGATC
BsaWI 2607 ACCGGA [AT]^CCGG[AT]
3022 TCCGGA
BsmBI 426 GAGACG CGTCTC(1/5)
3077 GAGACG
BsrDI 1820 GCAATG GCAATG(2/0)
3532 GCAATG
BsrFI 2567 GCCGGC [GA]^CCGG[CT]
3988 GCCGGC
BstBI 1961 TTCGAA TT^CGAA
3042 TTCGAA
Cfr10I 2567 GCCGGC [GA]^CCGG[CT]
3988 GCCGGC
EcoNI 1072 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4290 CCTGACTGAGG
FspI 1369 TGCGCA TGC^GCA
4339 TGCGCA
KasI 1378 GGCGCC G^GCGCC
3985 GGCGCC
MamI 1003 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2918 GATCTGGATC
NaeI 2567 GCCGGC GCC^GGC
3988 GCCGGC
NarI 1378 GGCGCC GG^CGCC
3985 GGCGCC
NcoI 1428 CCATGG C^CATGG
3162 CCATGG
NgoMIV 2567 GCCGGC G^CCGGC
3988 GCCGGC
NspV 1961 TTCGAA TT^CGAA
3042 TTCGAA
PflFI 325 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3661 GACGGAGTC
PflMI 2419 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3409 CCAGGTGGTGG
SalI 2099 GTCGAC G^TCGAC
3611 GTCGAC
SexAI 4038 ACCTGGT A^CC[AT]GGT
4215 ACCAGGT
SfiI 769 GGCCAACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
834 GGCCCATCCGGCC
SfoI 1378 GGCGCC GGC^GCC
3985 GGCGCC
SfuI 1961 TTCGAA TT^CGAA
3042 TTCGAA
SmaI 437 CCCGGG CCC^GGG
1631 CCCGGG
Tth111I 325 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3661 GACGGAGTC
Van91I 2419 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3409 CCAGGTGGTGG
XmaCI 437 CCCGGG C^CCGGG
1631 CCCGGG
XmaI 437 CCCGGG C^CCGGG
1631 CCCGGG
XmnI 1405 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3232 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII