##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
Aor51HI AscI AseI AsnI AviII
BamHI BbeI BciVI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BstBI BstUI ClaI
CpoI DraI DraIII Eco47III EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI MvnI NarI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfoI SfuI SgrAI SmaI
SphI Sse8387I SspBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 537 GGTACC G^GTACC
AflII 1185 CTTAAG C^TTAAG
AflIII 1873 ACATGT A^C[GA][CT]GT
AhdI 1615 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1755 GTGCAC G^TGCAC
ApaLI 1755 GTGCAC G^TGCAC
Asp718I 537 GGTACC G^GTACC
AspEI 1615 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1909 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 474 CTCGAG C^[CT]CG[GA]G
AvrII 3635 CCTAGG C^CTAGG
BalI 1291 TGGCCA TGG^CCA
BbrPI 3514 CACGTG CAC^GTG
BcgI 20 GCAGTAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 928 TGATCA T^GATCA
BfrI 1185 CTTAAG C^TTAAG
BglII 1066 AGATCT A^GATCT
BlnI 3635 CCTAGG C^CTAGG
BsaWI 2567 TCCGGT [AT]^CCGG[AT]
BsiEI 2672 CGGCCG CG[GA][CT]^CG
BsmBI 3915 CGTCTC CGTCTC(1/5)
BsoBI 474 CTCGAG C^[CT]CG[GA]G
BspLU11I 1873 ACATGT A^CATGT
BstEII 945 GGTTACC G^GT[ACGT]ACC
BstPI 945 GGTTACC G^GT[ACGT]ACC
EagI 2672 CGGCCG C^GGCCG
Eam1105I 1615 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2672 CGGCCG C^GGCCG
Eco52I 2672 CGGCCG C^GGCCG
EcoO65I 945 GGTTACC G^GT[ACGT]ACC
FbaI 928 TGATCA T^GATCA
KpnI 537 GGTACC GGTAC^C
MluNI 1291 TGGCCA TGG^CCA
MscI 1291 TGGCCA TGG^CCA
NheI 254 GCTAGC G^CTAGC
PaeR7I 474 CTCGAG C^TCGAG
PflFI 1909 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3514 CACGTG CAC^GTG
PmlI 3514 CACGTG CAC^GTG
ScaI 439 AGTACT AGT^ACT
SexAI 3082 ACCAGGT A^CC[AT]GGT
SfiI 2108 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 4386 TACGTA TAC^GTA
SpeI 1704 ACTAGT A^CTAGT
SspI 570 AATATT AAT^ATT
Tth111I 1909 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3171 TCTAGA T^CTAGA
XcmI 334 CCATTCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 474 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 1615 GACGCC G[GA]^CG[CT]C
1909 GACGCC
AocI 2001 CCTGAGG CC^T[ACGT]AGG
2952 CCTGAGG
ApaI 2473 GGGCCC GGGCC^C
4309 GGGCCC
BsaAI 3514 CACGTG [CT]AC^GT[GA]
4386 TACGTA
BsaHI 1615 GACGCC G[GA]^CG[CT]C
1909 GACGCC
BsmI 3560 GAATGC GAATGC(1/-1)
3477 GCATTC
BsrBI 477 GAGCGG CCGCTC(-3/-3)
1842 GAGCGG
BssSI 289 CACGAG CACGAG(-5/-1)
1805 CACGAG
Bst1107I 3132 GTATAC GTA^TAC
4346 GTATAC
BstZ17I 3132 GTATAC GTA^TAC
4346 GTATAC
Bsu36I 2001 CCTGAGG CC^T[ACGT]AGG
2952 CCTGAGG
Eco81I 2001 CCTGAGG CC^T[ACGT]AGG
2952 CCTGAGG
EcoRI 886 GAATTC G^AATTC
2091 GAATTC
HaeII 1821 AGCGCC [GA]GCGC^[CT]
2860 GGCGCT
Hin1I 1615 GACGCC G[GA]^CG[CT]C
1909 GACGCC
NaeI 577 GCCGGC GCC^GGC
954 GCCGGC
NgoMIV 577 GCCGGC G^CCGGC
954 GCCGGC
PpuMI 104 GGGACCT [GA]G^G[AT]CC[CT]
2377 GGGTCCC
StuI 2026 AGGCCT AGG^CCT
3904 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI