##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI ApaI AscI Asp700I Asp718I
AvaI AviII AvrII BamHI BbrPI
BcgI BlnI BsaAI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BssHII BstEII BstPI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HpaI KpnI KspI MfeI MluI
MroI MunI NotI PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 3215 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2823 GTGCAC G^TGCAC
ApaLI 2823 GTGCAC G^TGCAC
AspEI 3215 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5527 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 6229 CCTCAGC CCTCAGC(-5/-2)
BcnI 3936 CCCGG CC^[GC]GG
BglI 6574 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3908 TCCGGT [AT]^CCGG[AT]
BsrFI 52 GCCGGC [GA]^CCGG[CT]
BstXI 5564 CCAGTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
Eam1105I 3215 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 754 GAATTC G^AATTC
NaeI 52 GCCGGC GCC^GGC
NciI 3936 CCCGG CC^[GC]GG
NgoMIV 52 GCCGGC G^CCGGC
NheI 4386 GCTAGC G^CTAGC
NruI 1467 TCGCGA TCG^CGA
PflFI 5527 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3134 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3341 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 552 AGTACT AGT^ACT
Tth111I 5527 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3134 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 25 GGCGCC G[GA]^CG[CT]C
3737 GGCGCC
AflII 1584 CTTAAG C^TTAAG
3190 CTTAAG
AocI 1188 CCTCAGG CC^T[ACGT]AGG
5983 CCTGAGG
AseI 532 ATTAAT AT^TAAT
6451 ATTAAT
AsnI 532 ATTAAT AT^TAAT
6451 ATTAAT
BbeI 25 GGCGCC GGCGC^C
3737 GGCGCC
BfrI 1584 CTTAAG C^TTAAG
3190 CTTAAG
BglII 594 AGATCT A^GATCT
2115 AGATCT
BlpI 47 GCTGAGC GC^T[ACGT]AGC
4937 GCTTAGC
Bpu1102I 47 GCTGAGC GC^T[ACGT]AGC
4937 GCTTAGC
BsaBI 2134 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4114 GATAAGAATC
BsaHI 25 GGCGCC G[GA]^CG[CT]C
3737 GGCGCC
BsaI 6382 GAGACC GGTCTC(1/5)
1294 GGTCTC
BsgI 7316 CTGCAC GTGCAG(16/14)
5479 GTGCAG
BspMI 6794 ACCTGC ACCTGC(4/8)
6766 GCAGGT
BssSI 2453 CACGAG CACGAG(-5/-1)
6853 CACGAG
Bst1107I 623 GTATAC GTA^TAC
3611 GTATAC
BstAPI 5239 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5668 GCAAAGGGTGC
BstBI 2348 TTCGAA TT^CGAA
5789 TTCGAA
BstZ17I 623 GTATAC GTA^TAC
3611 GTATAC
Bsu36I 1188 CCTCAGG CC^T[ACGT]AGG
5983 CCTGAGG
CelII 47 GCTGAGC GC^T[ACGT]AGC
4937 GCTTAGC
DraIII 3270 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
7030 CACTCTGTG
DsaI 1835 CCATGG C^C[GA][CT]GG
6368 CCATGG
Eco81I 1188 CCTCAGG CC^T[ACGT]AGG
5983 CCTGAGG
EcoNI 2574 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4725 CCTATTTCAGG
HaeII 25 GGCGCC [GA]GCGC^[CT]
3737 GGCGCC
HgaI 661 GCGTC GACGC(5/10)
1473 GCGTC
Hin1I 25 GGCGCC G[GA]^CG[CT]C
3737 GGCGCC
KasI 25 GGCGCC G^GCGCC
3737 GGCGCC
MamI 2134 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4114 GATAAGAATC
NarI 25 GGCGCC GG^CGCC
3737 GGCGCC
NcoI 1835 CCATGG C^CATGG
6368 CCATGG
NdeI 4160 CATATG CA^TATG
7541 CATATG
NspV 2348 TTCGAA TT^CGAA
5789 TTCGAA
PshBI 532 ATTAAT AT^TAAT
6451 ATTAAT
SacI 4429 GAGCTC GAGCT^C
6148 GAGCTC
SapI 4426 GAAGAGC GCTCTTC(1/4)
5575 GCTCTTC
SfoI 25 GGCGCC GGC^GCC
3737 GGCGCC
SfuI 2348 TTCGAA TT^CGAA
5789 TTCGAA
SphI 1154 GCATGC GCATG^C
4946 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvI BciVI BclI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII