##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII BbeI BbrPI BcgI BglI
BglII BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspMI BssHII BstEII BstPI BstUI
CfoI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KspI
MluI MroI MvnI NarI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SgrAI SnaBI SphI Sse8387I StuI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 4223 GGTACC G^GTACC
AccI 2417 GTATAC GT^[AC][GT]AC
AflII 4155 CTTAAG C^TTAAG
ApaI 884 GGGCCC GGGCC^C
Asp718I 4223 GGTACC G^GTACC
AvaI 2623 CCCGGG C^[CT]CG[GA]G
BamHI 2395 GGATCC G^GATCC
BbvCI 2288 CCTCAGC CCTCAGC(-5/-2)
BciVI 456 GTATCC GTATCC(6/5)
BclI 863 TGATCA T^GATCA
BfrI 4155 CTTAAG C^TTAAG
BsaBI 858 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3935 ACCGGA [AT]^CCGG[AT]
BseRI 36 GAGGAG GAGGAG(10/8)
BsoBI 2623 CCCGGG C^[CT]CG[GA]G
BspHI 4616 TCATGA T^CATGA
BsrDI 1574 CATTGC GCAATG(2/0)
BsrFI 98 GCCGGC [GA]^CCGG[CT]
Bst1107I 2417 GTATAC GTA^TAC
BstAPI 1613 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2869 TTCGAA TT^CGAA
BstZ17I 2417 GTATAC GTA^TAC
Cfr10I 98 GCCGGC [GA]^CCGG[CT]
DraII 755 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 3774 GACTGATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 755 AGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 863 TGATCA T^GATCA
HpaI 4480 GTTAAC GTT^AAC
KpnI 4223 GGTACC GGTAC^C
MamI 858 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 98 GCCGGC GCC^GGC
NcoI 1396 CCATGG C^CATGG
NdeI 2330 CATATG CA^TATG
NgoMIV 98 GCCGGC G^CCGGC
NheI 1148 GCTAGC G^CTAGC
NspV 2869 TTCGAA TT^CGAA
PflMI 2085 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 755 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 163 CTGCAG CTGCA^G
RcaI 4616 TCATGA T^CATGA
SfuI 2869 TTCGAA TT^CGAA
SmaI 2623 CCCGGG CCC^GGG
Van91I 2085 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2839 TCTAGA T^CTAGA
XmaCI 2623 CCCGGG C^CCGGG
XmaI 2623 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 1921 ACATGT A^C[GA][CT]GT
3753 ACATGT
AlwNI 3770 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
4695 CAGTTCCTG
AocI 2169 CCTAAGG CC^T[ACGT]AGG
3794 CCTCAGG
Asp700I 720 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2712 GAAGGATTTC
AvrII 620 CCTAGG C^CTAGG
3626 CCTAGG
BalI 2572 TGGCCA TGG^CCA
3309 TGGCCA
BanI 110 GGCACC G^G[CT][GA]CC
4223 GGTACC
BlnI 620 CCTAGG C^CTAGG
3626 CCTAGG
BlpI 53 GCTCAGC GC^T[ACGT]AGC
351 GCTGAGC
Bpu1102I 53 GCTCAGC GC^T[ACGT]AGC
351 GCTGAGC
BspLU11I 1921 ACATGT A^CATGT
3753 ACATGT
BsrBI 39 GAGCGG CCGCTC(-3/-3)
145 GAGCGG
BssSI 382 CACGAG CACGAG(-5/-1)
1831 CACGAG
BstYI 900 AGATCC [GA]^GATC[CT]
2395 GGATCC
Bsu36I 2169 CCTAAGG CC^T[ACGT]AGG
3794 CCTCAGG
CelII 53 GCTCAGC GC^T[ACGT]AGC
351 GCTGAGC
DraI 1271 TTTAAA TTT^AAA
3610 TTTAAA
DraIII 1213 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2798 CACCTTGTG
Eco81I 2169 CCTAAGG CC^T[ACGT]AGG
3794 CCTCAGG
EcoRI 2662 GAATTC G^AATTC
4492 GAATTC
EcoRV 429 GATATC GAT^ATC
1941 GATATC
HincII 2762 GTTGAC GT[CT]^[GA]AC
4480 GTTAAC
HindII 2762 GTTGAC GT[CT]^[GA]AC
4480 GTTAAC
HindIII 723 AAGCTT A^AGCTT
3947 AAGCTT
MflI 900 AGATCC [GA]^GATC[CT]
2395 GGATCC
MluNI 2572 TGGCCA TGG^CCA
3309 TGGCCA
MscI 2572 TGGCCA TGG^CCA
3309 TGGCCA
MspA1I 4104 CAGCTG C[AC]G^C[GT]G
4406 CAGCTG
PvuII 4104 CAGCTG CAG^CTG
4406 CAGCTG
ScaI 1056 AGTACT AGT^ACT
2492 AGTACT
SexAI 481 ACCAGGT A^CC[AT]GGT
993 ACCTGGT
SpeI 1862 ACTAGT A^CTAGT
4439 ACTAGT
SspI 3720 AATATT AAT^ATT
4647 AATATT
XcmI 1396 CCATGGACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2575 CCAAAAGCATGATGG
XhoII 900 AGATCC [GA]^GATC[CT]
2395 GGATCC
XmnI 720 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2712 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MnlI MseI MslI
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I