##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI AspEI AspI BamHI BbeI
BbrPI BcgI BciVI BlpI Bpu1102I
BsaAI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FseI HaeII HpaI KasI KspI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 758 CGCG CG^CG
AcyI 1218 GACGCC G[GA]^CG[CT]C
AflII 4220 CTTAAG C^TTAAG
AvaI 2515 CTCGGG C^[CT]CG[GA]G
AviII 318 TGCGCA TGC^GCA
BclI 4031 TGATCA T^GATCA
BcnI 2800 CCGGG CC^[GC]GG
BfrI 4220 CTTAAG C^TTAAG
BsaBI 793 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1218 GACGCC G[GA]^CG[CT]C
BsaI 2486 GAGACC GGTCTC(1/5)
BsaWI 1011 ACCGGA [AT]^CCGG[AT]
BsiEI 1207 CGGCCG CG[GA][CT]^CG
BsoBI 2515 CTCGGG C^[CT]CG[GA]G
BspDI 1412 ATCGAT AT^CGAT
BspHI 1536 TCATGA T^CATGA
BsrDI 1076 CATTGC GCAATG(2/0)
BstUI 758 CGCG CG^CG
ClaI 1412 ATCGAT AT^CGAT
DrdI 197 GACTTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1207 CGGCCG C^GGCCG
EclXI 1207 CGGCCG C^GGCCG
Eco52I 1207 CGGCCG C^GGCCG
FbaI 4031 TGATCA T^GATCA
FspI 318 TGCGCA TGC^GCA
Hin1I 1218 GACGCC G[GA]^CG[CT]C
HindIII 3207 AAGCTT A^AGCTT
MaeII 1709 ACGT A^CGT
MamI 793 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 758 CGCG CG^CG
NciI 2800 CCGGG CC^[GC]GG
RcaI 1536 TCATGA T^CATGA
SapI 2174 GCTCTTC GCTCTTC(1/4)
ScaI 3622 AGTACT AGT^ACT
SfiI 2635 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 4220 CTTAAG C^T[CT][GA]AG
SpeI 1957 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 2086 GGTACC G^GTACC
3464 GGTACC
ApaI 1552 GGGCCC GGGCC^C
1632 GGGCCC
Asp700I 64 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3311 GAAGCCATTC
Asp718I 2086 GGTACC G^GTACC
3464 GGTACC
AvrII 2476 CCTAGG C^CTAGG
4044 CCTAGG
BbsI 250 GAAGAC GAAGAC(2/6)
1421 GTCTTC
BglII 1566 AGATCT A^GATCT
3691 AGATCT
BlnI 2476 CCTAGG C^CTAGG
4044 CCTAGG
BpuAI 250 GAAGAC GAAGAC(2/6)
1421 GTCTTC
BspLU11I 2206 ACATGT A^CATGT
4184 ACATGT
BsrBI 1324 CCGCTC CCGCTC(-3/-3)
2823 CCGCTC
BsrFI 869 GCCGGC [GA]^CCGG[CT]
1116 GCCGGC
BstXI 554 CCAGTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
802 CCACTTTGATGG
CfoI 319 GCGC GCG^C
3929 GCGC
Cfr10I 869 GCCGGC [GA]^CCGG[CT]
1116 GCCGGC
DraIII 551 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2667 CACTCTGTG
EcoRI 1691 GAATTC G^AATTC
2793 GAATTC
FauI 1155 CCCGC CCCGC(4/6)
1376 GCGGG
HhaI 319 GCGC GCG^C
3929 GCGC
HinP1I 319 GCGC G^CGC
3929 GCGC
HincII 419 GTTGAC GT[CT]^[GA]AC
2718 GTTGAC
HindII 419 GTTGAC GT[CT]^[GA]AC
2718 GTTGAC
KpnI 2086 GGTACC GGTAC^C
3464 GGTACC
NaeI 869 GCCGGC GCC^GGC
1116 GCCGGC
NcoI 2616 CCATGG C^CATGG
3287 CCATGG
NgoMIV 869 GCCGGC G^CCGGC
1116 GCCGGC
PflMI 550 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3287 CCATGGGCTGG
Van91I 550 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3287 CCATGGGCTGG
XcmI 1894 CCACAGCCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3992 CCACTTATATTTTGG
XmnI 64 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3311 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaII BalI BanI BanII
BbvCI BbvI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII